3,098 research outputs found
Direct replacement of antibodies with molecularly imprinted polymer (MIP) nanoparticles in ELISA - development of a novel assay for vancomycin
No full textA simple and straightforward technique for coating microplate wells with molecularly imprinted polymer nanoparticles (nanoMIPs) to develop ELISA type assays is presented here for the first time. NanoMIPs were synthesized by a solid phase approach with immobilized vancomycin (template) and characterized using Biacore 3000, dynamic light scattering and electron microscopy. Immobilization, blocking and washing conditions were optimized in microplate format. The detection of vancomycin was achieved in competitive binding experiments with a HRP-vancomycin conjugate. The assay was capable of measuring vancomycin in buffer and in blood plasma within the range 0.001-70 nM with a detection limit of 0.0025 nM (2.5 pM). The sensitivity of the assay was three orders of magnitude better than a previously described ELISA based on antibodies. In these experiments nanoMIPs have shown high affinity and minimal interference from blood plasma components. Immobilized nanoMIPs were stored for 1 month at room temperature without any detrimental effects to their binding properties. The high affinity of nanoMIPs and the lack of a requirement for cold chain logistics make them an attractive alternative to traditional antibodies used in ELIS
2C y los territorios de la arquitectura de la razon
No full textNelle pagine della Rivista 2C Construccion de la ciudad Carlos Marti Aris propone una costellazione semantica imperniata sui concetti di Realismo, Classicismo e Razionalità: una costellazoine ceh riprende dal dibattito italiano e dalla posizione di Rossi e che trasferisce nel dibattito spagnolo in un passaggio cruciale della fine del XX secoloIn the pages of the magazine 2C Construccion de la ciudad Carlos Marti Aris proposes a semantic constellation hinging on the concepts of Realism, Classicism and Rationality: a constellation that takes from the Italian debate and Rossi's position and transfers it to the Spanish debate in a crucial passage at the end of the 20th centur
CO2 transoral microsurgery for supraglottic squamous cell carcinoma
No full textThe present study analyzed the results of the endoscopic approach to T1, T2 and selected T3 supraglottic carcinoma with the aim of reviewing functional and oncologic outcomes after different types of endoscopic supraglottic laryngectomies. This is a retrospective clinical study of 42 consecutive patients (mean age of 61.8 years, 33 males, 9 females) treated by the senior author for supraglottic squamous cell carcinoma with a transoral CO2 laser approach and reviewed from November 2010 to September 2017. Surgical procedures were classified according to the European Laryngological Society. In addition to the standardized transoral supraglottic laryngectomies, we introduced a modified type IVb by sparing the inferior third of the arytenoid if not directly involved in the tumor. Swallowing was evaluated with the Swallowing Performance Status Scale reported by the Multinational Association of Supportive Care in Cancer/International Society of Oral Oncology. Survival probabilities were estimated using Kaplan-Meier curves. Two type I, 2 type IIa, 2 type IIb, 3 type IIIa, 12 type IIIb, 13 type IVa, 3 type modified IVb, and 5 type IVb supraglottic laryngectomies were performed. Twenty-one patients (50%) underwent primary neck dissection. The pathologic TNM classification according to the 8th edition of the American Joint Committee on Cancer system was as follows: 9 pT1cN0, 2 pT1N0, 1 pT1N1, 7 pT2cN0, 1 rypT2cN0, 9 pT2N0, 4 pT2N1, 2 ypT2N1, 2 pT3cN0, 2 rypT3cN0, 1 pT3N1, and 2 pT3N2b. Mean follow-up was 3.4 years (range of 9 months to 6 years). According to the Kaplan–Meier analysis, 5-year disease-specific survival, local-relapse-free survival, nodal-relapse-free survival, overall laryngeal preservation and overall survival of patients without previous head and neck radiotherapy/open surgery were 100%, 95.2%, 87.8%, 100%, and 64.6%, respectively. Patients who underwent type I, IIa, and IIb resections (n = 6) started oral feeding the day after surgery, patients who underwent type III-IVb modified resections (n = 31) started oral feeding 3–4 days after surgery, and patients who underwent standard type 4b resections (n = 5) started oral feeding 7 days after surgery. Three months after surgery, patients without a clinical history of previous head and neck radiotherapy/open surgery who underwent type III, IVa, and modified IVb resections showed significantly better swallowing compared to patients who underwent standard type IVb resection: grade 4–6 impairment of swallowing in 8 and 66.7% of cases, respectively (p = 0.006072); patients with a clinical history of previous head and neck radiotherapy/open surgery who underwent type III, IVa, and modified IVb resections showed not statistically significant better swallowing compared to patients who underwent standard type IVb resection: grade 4–6 impairment of swallowing at 3 months in 16.7% and 50% of cases, respectively (p = 0.23568). Transoral CO2 laser supraglottic laryngectomy is an oncologic sound alternative to traditional open neck surgery and chemo-radiotherapy. Recovery of swallowing is significantly worsened after total resection of the arytenoid. Modified type IVb procedure leaving intact, when possible, the inferior third of the arytenoid and consequently the glottic competence, improves functional outcome
Il passato degli 'antichi'
No full textAlcuni esempi di come gli antichi pensavano e concettualizzavano l'antico, da Platone a Senec
3D printing of potassium sodium niobate by binder jetting: Printing parameters optimisation and correlation to final porosity
No full textBinder Jetting (BJT) is a non-fusion-based Additive Manufacturing (AM) technique. It consists of the selective deposition of a liquid binder to join powder particles, thereby enabling the creation of near-net-shaped parts. In this study, the main printing parameters correlated to the binder distribution and infiltration (binder saturation, binder set time, drying time, and target bed temperature) were optimised to improve the precision of green parts printed with potassium sodium niobate (KNN) powder. The optimisation procedure was conducted using the Taguchi statistical method. An L9 orthogonal array with four factors of control at three levels each was employed. The analysis showed that the drying time had the greatest influence on the precision of green parts, followed by binder saturation and target bed temperature. Binder set time did not seem to affect the results. Dimensional analysis, microstructural and piezoelectric characterisation of parts densified by pressureless sintering were conducted. The highest average relative density exceeded 80% for the specimens printed with the lower binder saturation. Piezoelectric properties exhibit more complex behaviour. The prolonged infiltration of larger binder volumes is correlated to higher g33, thus FOMh and FOM33, and lower values for ε33^T. On the other hand, d33 does not display a specific dependence on density or printing parameters. The results of this study indicate that BJT can be used to fabricate high-precision KNN components with good piezoelectric properties. The optimisation of printing parameters is essential to achieve the desired results.Binder Jetting (BJT) is a non-fusion-based Additive Manufacturing (AM) technique. It consists of the selective deposition of a liquid binder to join powder particles, thereby enabling the creation of near-net-shaped parts. In this study, the main printing parameters correlated to the binder distribution and infiltration (binder saturation, binder set time, drying time, and target bed temperature) were optimised to improve the precision of green parts printed with potassium sodium niobate (KNN) powder. The optimisation procedure was conducted using the Taguchi statistical method. An L9 orthogonal array with four factors of control at three levels each was employed. The analysis showed that the drying time had the greatest influence on the precision of green parts, followed by binder saturation and target bed temperature. Binder set time did not seem to affect the results. Dimensional analysis, microstructural and piezoelectric characterisation of parts densified by pressureless sinterin..
Additive manufacturing of lead-free KNN by binder jetting
No full textAdditive manufacturing of lead-free piezoceramics is of great interest, given the large request of application-oriented designs with optimal performances and reduced material consumption. Binder Jetting (BJ) is an additive manufacturing technique potentially suited to the production of ceramic components, however the number of feasibility studies on BJ of piezoceramics is extremely limited and totally lacking in the case of sodium-potassium niobate (KNN). In this work, as-synthesised powders are employed in the BJ 3D printing process. Microstructural properties, such as porosity, grain size distributions, and phase composition are studied by SEM, XRD and MIP (Mercury Intrusion Porosimetry) and compared to die-pressed pellets. Analyses reveal considerable residual porosity (~40%) regardless of the printing parameters, with a weak preferential orientation parallel to the printing plane. The piezoelectric characterization demonstrates an outstanding d33 value of 80–90 pC N−1. Finally, Figures of Merits for the employment as porous piezoceramics in the direct mode are presented
Coaching early career teachers in urban elementary schools: A mixed method study
No full textCoaching for urban early career teachers (ECTs) offers promise and aligns with features of effective professional development to support the implementation of evidence-based practices. However, the functional components and key elements of coaching and coach supervision are not well specified in the literature. The goal of the current study was to examine adherence and feasibility of a coaching intervention designed to provide urban ECTs with concentrated support in classroom management and engaging learners—two instructional domains that are robust predictors of attrition (Ingersoll and Strong in Rev Educ Res 81:201–233, 2011). Coaches (n = 6) worked with ECTs (n = 15) in three urban, high-poverty elementary schools during the 2-year intervention. A mixed-method design was employed, such that qualitative data (i.e., semi-structured interviews) and quantitative data (i.e., adherence measures) were collected concurrently, remained independent during analyses, and were integrated during interpretation (Creswell and Clark in Designing and conducting mixed methods research. Sage, Thousand Oaks, 2007). Findings revealed that ECTs generally received the intended frequency and duration of coaching but with fewer opportunities for post-conferences. Coach supervision, on average, was delivered with intended frequency, with variability across coaches. Thematic analyses highlighted coach provision of emotional and instrumental support, emphasized consistent coaching as critical, and that time was a significant barrier to ECT participation in coaching. Supervision promoted social support among coaches and provided opportunities to adapt the model to ECT needs.Peer reviewe
Communautés et écriture en ligne. Histoire, devenir, un dialogue
No full textIn this interview, Elisa Bricco talks to writer Benoît Vincent about
the transformations that have taken place over the last twenty years
as a result of the technological turn in literary production. We look
at the repercussions of technology’s hold over literary production, and
in particular at the development of the notion of author in the context
of collective writing. The example of the General Instin project will
be used to highlight some of the issues raised by digital technology in
relation to the production of literary texts.Dans cet entretien, Elisa Bricco discute avec l’écrivain Benoît Vincent
sur les transformations qui sont survenues dans les vingt dernières
années à cause du tournant technologique de la production littéraire. On
s’interroge sur les répercussions de l’emprise de la technologie sur le
faire littéraire et notamment sur le développement de la notion d’auteur
dans le contexte de l’écriture collective. L’exemple du projet Général
Instin servira pour mettre en lumières quelques enjeux du numérique
par rapport à la production de textes littéraires
Communautés et écriture en ligne. Histoire, devenir, un dialogue
No full textIn this interview, Elisa Bricco talks to writer Benoît Vincent about the transformations that have taken place over the last twenty years as a result of the technological turn in literary production. We look at the repercussions of technology’s hold over literary production, and in particular at the development of the notion of author in the context of collective writing. The example of the Général Instin project will be used to highlight some of the issues raised by digital technology in relation to the production of literary texts.Dans cet entretien, Elisa Bricco discute avec l’écrivain Benoît Vincent sur les transformations qui sont survenues dans les vingt dernières années à cause du tournant technologique de la production littéraire. On s’interroge sur les répercussions de l’emprise de la technologie sur le faire littéraire et notamment sur le développement de la notion d’auteur dans le contexte de l’écriture collective. L’exemple du projet Général Instin servira pour mettre en lumières quelques enjeux du numérique par rapport à la production de textes littéraires
Comment on Bandelli and Porcelli/1 - Against Moral Panic, in Defence of Data
No full textElisa Giomi’s commentary is an answer to Bandelli and Porcelli’s essay “Femicide in Italy. ‘Femminicidio,’ Moral Panic and Progressivist Discourse” [2016]. Although Giomi assumes that Bandelli and Porcelli effectively illustrate the instrumentalization of lethal violence against women in Italian political discourse, yet she criticizes their reading of the “femminicidio narrative” as a phenomenon of moral panic that locates the threat in the typical Italian heterosexual family/couple: all the available empirical findings confirm that these are precisely the contexts where lethal aggressions against women most frequently occur. The author also disagrees with the authors’ conclusion that the femminicidio narrative authorized “the application of a gender paradigm to the official reading of domestic/partner violence” in 2012/13 Italian public discourse. Finally, she contends that in their essay the “feminist discourse” is represented in a stereotypical way, one that is instrumental in depicting it as a hegemonic discourse
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