117,641 research outputs found

    The Virome of ‘Lamon Bean’: Application of MinION Sequencing to Investigate the Virus Population Associated with Symptomatic Beans in the Lamon Area, Italy

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    ‘Lamon bean’ is a protected geographical indication (PGI) for a product of four varieties of bean (Phaseolus vulgaris L.) grown in a specific area of production, which is located in the Belluno district, Veneto region (N.E. of Italy). In the last decade, the ‘Lamon bean’ has been threatened by severe virus epidemics that have compromised its profitability. In this work, the full virome of seven bean samples showing different foliar symptoms was obtained by MinION sequencing. Evidence that emerged from sequencing was validated through RT-PCR and ELISA in a large number of plants, including different ecotypes of Lamon bean and wild herbaceous hosts that may represent a virus reservoir in the field. Results revealed the presence of bean common mosaic virus (BCMV), cucumber mosaic virus (CMV), peanut stunt virus (PSV), and bean yellow mosaic virus (BYMV), which often occurred as mixed infections. Moreover, both CMV and PSV were reported in association with strain-specific satellite RNAs (satRNAs). In conclusion, this work sheds light on the cause of the severe diseases affecting the ‘Lamon bean’ by exploitation of MinION sequencing

    The Virome of ‘Lamon Bean’: Application of MinION Sequencing to Investigate the Virus Population Associated with Symptomatic Beans in the Lamon Area, Italy

    No full text
    ‘Lamon bean’ is a protected geographical indication (PGI) for a product of four varieties of bean (Phaseolus vulgaris L.) grown in a specific area of production, which is located in the Belluno district, Veneto region (N.E. of Italy). In the last decade, the ‘Lamon bean’ has been threatened by severe virus epidemics that have compromised its profitability. In this work, the full virome of seven bean samples showing different foliar symptoms was obtained by MinION sequencing. Evidence that emerged from sequencing was validated through RT-PCR and ELISA in a large number of plants, including different ecotypes of Lamon bean and wild herbaceous hosts that may represent a virus reservoir in the field. Results revealed the presence of bean common mosaic virus (BCMV), cucumber mosaic virus (CMV), peanut stunt virus (PSV), and bean yellow mosaic virus (BYMV), which often occurred as mixed infections. Moreover, both CMV and PSV were reported in association with strain-specific satellite RNAs (satRNAs). In conclusion, this work sheds light on the cause of the severe diseases affecting the ‘Lamon bean’ by exploitation of MinION sequencing

    Effect of Finnsheep crossbreeding on Lamon sheep performance: in vivo traits

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    The objective of this trial was the comparison of the in vivo traits of Lamon (L), a local meat breed of the Eastern Italia Alps, and Finnsheep X Lamon (F x L) fattening lambs. Forty-one lambs (25 L and 16 F x L) of both sexes were weaned at 8 weeks of age and fattened for 14 weeks. The diet (11,6 MJ/kg d.m.M.E.) consisted of maize silage ad lib., 200 g/d of dried sugar beet pulp, 150 g/d of soybean meal and 30 g/d supplement. F x L lambs grew slightly more than L lambs (197 vs 176 g/d; P .1) while M.E. requirements for growth, estimated assuming a maintenance requirement of.44 MJ • d-1 • kg-1 • L.W.-75, resulted higher (+7 %) for F x L than for L lambs (2.18 vs 2.04 MJ • kgDG • kg-1 • L.W.-75; P < .1). The ram-lambs showed superior growth potential and feed efficiency in respect to the ewe-lambs. In conclusion it appears that crossbreeding with Finnsheep is not detrimental to the in vivo performance of fattening Lamon lambs except for a slight increase of the energy requirements for growth

    Improving the prediction of environmental fate of engineered nanomaterials by fractal modelling

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    A critical analysis of the available engineered nanomaterials (ENMs) environmental fate modelling approaches indicates that existing tools do not satisfactorily account for the complexities of nanoscale phenomena. Fractal modelling (FM) can complement existing kinetic fate models by including more accurate interpretations of shape and structure, density and collision efficiency parameters to better describe homo- and heteroaggregation. Pathways to including hierarchical symmetry concepts and a route to establishing a structural classification of nanomaterials based on FM are proposed.A critical analysis of the available engineered nanomaterials (ENMs) environmental fate modelling approaches indicates that existing tools do not satisfactorily account for the complexities of nanoscale phenomena. Fractal modelling (FM) can complement existing kinetic fate models by including more accurate interpretations of shape and structure, density and collision efficiency parameters to better describe homo- and heteroaggregation. Pathways to including hierarchical symmetry concepts and a route to establishing a structural classification of nanomaterials based on FM are proposed. (C) 2016 Published by Elsevier Ltd

    EFFECT OF FINNSHEEP CROSSBREEDING ON LAMON SHEEP PERFORMANCE - POST-MORTEM TRAITS

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    This experiment was conducted in order to study the effects of crossbreeding of the local breed Lamon (L) with Finnsheep (F),on the post-mortem performance of F, lambs (F x L). Nine L and 8 F x L ram-lambs were fattened with a diet (11.6 MJ M.E./kg d.m.) based on maize silage, dried sugar beet pulp and soybean meal, and slaughtered at 40.5 ± 5.9 kg live weight at the age of 22 weeks. Genotype did not affect dressing percentage on empty body weight (E.B.W.) but the crossbred lambs showed a lower incidence of the pelt (18,4 vs 20.2 % on E.B.W.;
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