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Indagine epidemiologica sulla presenza di Prototheca spp. in allevamenti di bovine da latte
No full textCTX-M1 ESBL-producing Klebsiella pneumoniae subsp. pneumonia isolated from cases of bovine mastitis
No full textEpidemiology and genotyping of Candida rugosa strains responsible for persistent intramammary infections in dairy cows
Get PDFThe present study was undertaken during an outbreak of clinical and subclinical mastitis in 14 dairy cows caused by Candida rugosa, in which high somatic cell counts were seen and cases did not respond to antibiotic treatment. Intramammary infection cured spontaneously in 10 cows, whereas 4 cows were culled as a result of persistent infections. Repeated sampling of these cows and biomolecular analysis of the isolates showed that the infections were caused by the same genotype, even over a period of 2 lactations. Random amplification of the genome of C. rugosa milk isolates gave 3 different DNA banding patterns (genotypes G1, G2, and G3). Viable cells of C. rugosa were also isolated from various environmental sources and were present in high concentrations in total mixed ration samples, which could be considered the primary source of diffusion of viable yeast cells in the environment, as demonstrated by genotyping. The proven capacity of these microorganisms to survive in the environment of the cow, such as the total mixed ration, bedding, water, and cow skin, and to cause persistent intramammary infections highlights the importance of mycotic spread in dairy herds
Spread of MRSA strains within a dairy farm strictly related to a pig holding
No full textStaphylococcus aureus is an ubiquitous and important pathogen causing diseases in humans and animals. It is a major and contagious pathogen in dairies. Methicillin-resistant S. aureus (MRSA)strains arec haracterized by the mecA gene coding for a modified penicillin-binding protein (PBP). The derived reduced susceptibility to antibiotics is a major concern in medicine and is gradually becoming important for livestock animals too. Pigs and poultry are well-known MRSA reservoir. Animals caretakers working in those farms are at high and demonstrated risk to be contaminated (3). Dairy cows can be positive too, mastitis can be caused by MRSA at the same extent of MSSA and prevalence can be at an unexpected level (5). Relatedness with swine farms could represent a source of infection for cows through environmental contamination or human carriage of caretakers and milkers. In this study we describe the contamination of milk with MRSA strains in a dairy herd which is located within few meters from a farrow-to-finish pig holding, under the same property. Genetic
comparison between strains isolated from pigs, cows, personnel and environment has been performed through the amplification of the 16S-23S rRNA intergenic spacer (RS-PCR) a
technique suitable to (i) classify strains with high virulence and diffusivity (2) and to (ii) trace the possible path of infection
Prevalence of intramammary infections in heifer around calving in 40 dairy herds in the West of France
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Produzioni di ß-lattamasi a spettro esteso in ceppi di E. coli isolati da mastiti cliniche bovine
No full textIl ruolo della mungitura meccanica nella sanità della mammella bovina: aspetti impiantistici e gestionali
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The acute phase protein response to intramammary infection in water buffalo (Bubalus bubalis)
No full textAcute phase proteins are a group of serum proteins that change their concentration in animals following infection, inflammation or stress. Serum amyloid A (SAA) and α1-acid glycoprotein (AGP) are regarded as acute phase proteins in bovine, and their concentrations increase in blood and milk from cows during mastitis.
In this study we determined the concentration of SAA and AGP in serum and milk collected from healthy water buffaloes (Bubalus bubalis) and buffaloes with intramammary infection (IMI). The nucleotide sequences of SAA and AGP were determined by cDNA sequencing and showed homology of 9% and 95% respectively with bovine one.
The concentration of SAA and AGP in serum (34 samples) or milk (134 samples) were analyzed by established immunologic methods utilizwed for bovine, after validation of the cross-reactivity of the antibodies included in the kit with the buffalo proteins.
The concentration of serum and milk AGP was under the detection limit of the kit, and was therefore undetectable. SAA cut-off values were assumed to be 4.1mg/l in serum and 1.4 mg/l in milk as in bovine. Data collected showed that the concentration of serum from all of the buffaloes with IMI (17.8 mg/l ± 9.5 s.e., p<0.001). results in milk showed that in healthy buffaloes the protein was not detectable, but when IMI is present SAA was detectable in all samples with mean concentration of 4.1 mg/l ± 0.37 s.e. (p<0.001).
In conclusion, SAA concentration below the detection limit were considered as good indicators of healthy udder quarters. A substantial variation in SAA concentration in milk and serum was observed in buffalos with bacterial infection. For this reason SAA can be considered as a good marker for early mastitis diagnoses
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