173 research outputs found
Monitoring the presence of Chikungunya virus in Aedes albopictus population in Pisa (Tuscany), Italy,
No full textCo-infection with SARS-CoV-2 omicron BA.1 and BA.2 subvariants in a non-vaccinated woman
No full textHypothesis: Central nervous system delivery of cyclosporine A for therapy of progressive multifocal leukoencephalopathy
No full textHIV-1 NAT minipool during the pre-seroconversion window period: Detection of a repeat blood donor
No full textBackground: The introduction of nucleic acid amplification technology (NAT) for screening pooled or individual donations remarkably improved the safety of blood products. The size of mini-pooled NAT is considered critical for identification of HIV-1 infected donors during preseroconversion phase of infection. We describe a case of HIV-1 infection in a serologically negative repeat blood donor identified by 16 minipool (MP) NAT. Materials and Methods: The donation was tested by Roche Cobas AmpliScreen HIV-1 Test with manual extraction (MultiPrep Specimen Processing Procedure). The sensitivity of different MP sizes was observed. Serial samples of infected donor were examined with different third and fourth generation HIV-1 serological assays. Results: In the index donation viral load was 515 copies/ml corresponding to about 50 IU when diluted in 16 MP. Abbott third and fourth generation EIA tests detected the seroconversion four days later the index donation. Conclusion: The report emphasizes the relevance of a very small size of MP to really reduce the window serologic phase of current EIA test by HIV-1 NAT test. © 2005 Blackwell Publishing
Prevalenza e caratterizzazione molecolare del gemycirculavirus in soggetti sani e immunocompromessi
No full textPARTIAL NUCLEOTIDE SEQUENCING OF 6 SUBTYPE 2C HEPATITIS-C VIRUSES DETECTED IN ITALY
No full textThe great majority of 121 hepatitis C virus (HCV) isolates obtained from 117 Italian patients with community-acquired infection could readily be typed by genotype-specific PCR. Subtype Ib was dominant (74 isolates); subtypes 2b, 2a, and la followed, with 19, 14, and 8 isolates, respectively. The six isolates that remained untyped by this method were classified as subtype 2c on the basis of sequence analysis of PCR amplicons obtained from the core and NS5 genes. These findings indicate that HCV subtype 2c has a relatively high prevalence in Italy. Sequencing the core region from positions 160 to 259 is sufficient to distinguish subtype 2c from other known HCV genotypes
[Hyperacute complications of hepato-colic amebiasis. Apropos of a case of cecal perforation]
No full textdCTP misincorporation in a Chinese hamster mutator phenotype: the role of GGA genetic context
No full textClone CSA7 is a CHEF18 hamster cell line that shows an increased intracellular accumulation of dCTP. To localize the mutations that accumulate spontaneously in a functional gene of such a mutator phenotype, independent CSA7 mutants of the hypoxanthine-guanine phosphoribosyl transferase (hprt) gene were isolated and screened by a polymerase chain reaction-single strand conformation polymorphism technique. Sixty-two percent of mutants produced detectable changes of the strand migration profile and the mutations were preferentially localized in the exons 3 (31%) and 6 (62%). The sequencing of such exons revealed that the rate of C base incorporation was the major mutation pathway and that the A base of a GGA sequence was the preferential site of misincorporation
- …
