185 research outputs found
La probabilità, ancella e regina dell’analisi matematica. (Prendere sul serio Paul Lockhart, a ogni livello dell’insegnamento.)
No full textThe central proline rich region of POB1/REPS2 plays a regulatory role in epidermal growth factor receptor endocytosis by binding to 14-3-3 and SH3 domain-containing proteins
No full textBackground. The human POB1/REPS2 (Partner of RalBP1) protein is highly conserved in mammals where it has been suggested to function as a molecular scaffold recruiting proteins involved in vesicular traffic and linking them to the actin cytoskeleton remodeling machinery. More recently POB1/REPS2 was found highly expressed in androgen-dependent prostate cancer cell lines, while one of its isoforms (isoform 2) is down regulated during prostate cancer progression. Results. In this report we characterize the central proline rich domain of POB1/REPS2 and we describe for the first time its functional role in receptor endocytosis. We show that the ectopic expression of this domain has a dominant negative effect on the endocytosis of activated epidermal growth factor receptor (EGFR) while leaving transferrin receptor endocytosis unaffected. By a combination of different approaches (phage display, bioinformatics predictions, peptide arrays, mutagenic analysis, in vivo co-immunoprecipitation), we have identified two closely spaced binding motifs for 14-3-3 and for the SH3 of the proteins Amphiphysin II and Grb2. Differently from wild type, proline rich domains that are altered in these motifs do not inhibit EGFR endocytosis, suggesting that these binding motifs play a functional role in this process. Conclusion. Our findings are relevant to the characterization of the molecular mechanism underlying the involvement of POB1/REPS2, SH3 and 14-3-3 proteins in receptor endocytosis, suggesting that 14-3-3 could work by bridging the EGF receptor and the scaffold protein POB1/REPS2. © 2008 Tomassi et al; licensee BioMed Central Ltd
"In fornacem ignis ardentem". Osservazioni sulla rappresentazione della fornace nei sarcofagi paleocristiani con la scena del vivicomburium
No full textAuxin, photoperiod and putrescine affect flower neoformation in normal and rolB-transformed tobacco thin cell layers
No full textAuxin, photoperiod and putrescine are factors involved in the control of the reproductive phase of the flowering process in planta. The aim of this study was to investigate whether auxin is able, in the absence of other exogenous hormones, to stimulate flower neoformation in Nicotiana tabacum L, thin cell layers excised from flowering plants, whether an interaction exists between the hormone and the length of the light treatment per day, and whether exogenous putrescine interferes with auxin and light in the realization of the flowering programme. Since tissues transformed with rolB gene show an increased binding capacity of auxin, the response of rolB-explants was compared with that of the wild type. Thin cell layers from floral branches were cultured in the presence of a wide range (0-10 mu M) of indoleacetic acid alone, under various phototreatments (continuous darkness, 8 h light/day, and 16 h light/day). Floral, vegetative and rooting responses were favoured in the transgenic explants. Flower neoformation was stimulated by long days and by exogenous auxin (1 mu M). In rolB-thin cell layers flowering also occurred in the most unfavourable culture conditions (i.e. continuous darkness combined with either hormone-free medium or 10 mu M auxin). Long days and 1 mu M auxin also favoured caulogenesis, though to a lesser extent than flower neoformation. Rhizogenesis occurred in rolB-explants only and was induced by exogenous auxin and favoured by continuous darkness, In both wild type and rolB-explants, exogenous putrescine (1 mM) reduced flower neoformation in the presence of 1 mu M auxin and long days. (C) Elsevier, Paris
An inquiry into urban peripheries, socio-economic distress and vote. The cases of Bologna, Florence and Rome
No full textThe weakening of traditional parties and of their territorial rooting which has occurred over the last decades has brought back the scholarly interest on the local dynamics, namely, on the social and political transformations involving local areas. An increasing number of scholars has therefore focused on the «peripheries», those urban areas that are traditionally associated with a high level of socio-economic distress, wherein inhabitants feel themselves as economically disadvantaged, socially marginalized and politically excluded. This paper is part of such research strand by investigating the variation in the electoral results in three Italian cities – Bologna, Florence, and Rome – in relation to the spatial distance from the urban centre and to the socio-economic distress. More notably, the paper answers two main questions: a) are the most distant areas from the historical centre also those with a higher level of socio-economic distress? b) what kind of relationship is there between voting and socio-economic distress, and how does such relation change over the post-crisis years? To answer these questions, we consider the results of four elections (parliamentary elections 2008, 2013, 2018; European elections 2019) in the three cities investigated, which are similarly characterized by an electoral decline of the Pd (but also of the Pdl/FI) to the benefit of M5s and League (and in part also of FdI), besides being located in central Italy. By using an original dataset combining socio-economic and electoral variables at the district level, the article analyzes the variation in the electoral support for the main Italian political parties, with a particular focus on both mainstream (Pd and Pdl/FI) and antiestablishment parties (M5s and League)
Inchiesta su periferie urbane, disagio socio-economico e voto. I casi di Bologna, Firenze e Roma
No full textThe weakening of traditional parties and of their territorial rooting which has occurred over the last decades has brought back the scholarly interest on the local dynamics, namely, on the social and political transformations involving local areas. An increasing number of scholars has therefore focused on the «peripheries», those urban areas that are traditionally associated with a high level of socio-economic distress, wherein inhabitants feel themselves as economically disadvantaged, socially marginalized and politically excluded. This paper is part of such research strand by investigating the variation in the electoral results in three Italian cities – Bologna, Florence, and Rome – in relation to the spatial distance from the urban centre and to the socio-economic distress. More notably, the paper answers two main questions: a) are the most distant areas from the historical centre also those with a higher level of socio-economic distress? b) what kind of relationship is there between voting and socio-economic distress, and how does such relation change over the post-crisis years? To answer these questions, we consider the results of four elections (parliamentary elections 2008, 2013, 2018; European elections 2019) in the three cities investigated, which are similarly characterized by an electoral decline of the Pd (but also of the Pdl/FI) to the benefit of M5s and League (and in part also of FdI), besides being located in central Italy. By using an original dataset combining socio-economic and electoral variables at the district level, the article analyzes the variation in the electoral support for the main Italian political parties, with a particular focus on both mainstream (Pd and Pdl/FI) and antiestablishment parties (M5s and League)
Speed sensorless control based on Luenberger observer for DC motors
No full textThis paper provides a technical analysis of the sensorless method using observer Luenberger for speed determination for controlling DC motor drives using a current sensor. The operation of the sensorless speed control of a DC motor was verified in MATLAB/Simulink and then experimentally implemented on setup hardware to make a comparison between the Luenberger estimated value and the real one using a speed meter keyed on the motor. From the data, it was possible to obtain the measurement error at various operating speeds
Hemp (Cannabis sativa L., Kompolti cv.) and Hop (Humulus lupulus L., Chinook cv.) Essential Oil and Hydrolate: HS-GC-MS Chemical Investigation and Apoptotic Activity Evaluation
No full textIn this study, essential oils (EOs) and hydrolates (Hys) from Italian hemp (Cannabis sativa L. Kompolti cv.) and hop (Humulus Lupulus L., Chinook cv.) supply chains were chemically characterized and tested to investigate their apoptotic potential for the first time. Headspace–Gas Chromatography–Mass Spectrometry (HS-GC-MS) techniques were performed to describe their volatile chemical profile, highlighting a composition rich in terpene derivatives such as monoterpenes and sesquiterpenes among which β-myrcene, limonene, β-caryophyllene and α-humulene were the main constituents of EOs; in contrast, linalool, cis-p-menth-2,8-dien-1-ol, terpinen-4-ol, α-terpineol, caryophyllene oxide, and τ-cadinol were found in the Hys. The cytotoxicity activity on human leukemia cells (HL60), human neuroblastoma cells (SH-SY5Y), human metastatic adenocarcinoma breast cells (MCF7), human adenocarcinoma breast cells (MDA), and normal breast epithelial cell (MCF10A) for the EOs and Hys was studied by MTT assay and cytofluorimetric analysis and scanning and transmission electron microscopy were performed to define ultrastructural changes and the mechanism of cells death for HL 60 cells. An induction of the apoptotic mechanism was evidenced for hemp and hop EOs after treatment with the corresponding EC(50) dose. In addition, TEM and SEM investigations revealed typical characteristics induced by the apoptotic pathway. Therefore, thanks to the integration of the applied methodologies with the used techniques, this work provides an overview on the metabolomic profile and the apoptotic potential of hemp and hop EOs and, for the first time, also of Hys. The findings of this preliminary study confirm that the EOs and Hys from Cannabis and Humulus species are sources of bioactive molecules with multiple biological effects yet to be explored
Due aurighi d’eccezione: il Faraone ed Elia. Intorno all’immagine del carro nei sarcofagi tardoantichi a soggetto biblico
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