88 research outputs found

    Effects of weight loss while feeding a moderate-protein, high-fiber diet on body composition, voluntary physical activity, and blood metabolite profiles in overweight cats

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    Obesity is a major nutritional disorder in cats and is associated with several comorbidities and reduced life span. The primary objectives of this study were to determine the effects of feeding a moderate-protein, high-fiber diet on BW loss, voluntary physical activity levels, body composition, and blood metabolite profiles in overweight cats. During a 4-wk baseline period, 8 adult neutered male domestic shorthair cats (mean BW = 7.7±0.4 kg, mean BCS = 7.6/9) were fed to maintain BW. For 18 wk following baseline, food intake was adjusted to allow cats to lose weight at a rate of ~1.5% BW/wk. Cats were group-housed for 20 h/d and individually housed for two, 2-h periods each day for feedings. Daily food intake, twice-weekly BW, and weekly BCS were recorded throughout the study. Voluntary physical activity was measured over a 7-d period at wk 0, 6, 12, and 18 using Actical® activity monitors. Dual energy X-ray absorptiometry (DEXA) scans were taken at wk 0, 4, 8, 12, and 16 to estimate body composition. Overnight fasted blood samples were collected at wk 0, 1, 2, 4, 8, 12, and 16. The University of Illinois Institutional Animal Care and Use Committee approved all procedures. As expected, mean BW (7.7±0.4 vs. 6.2±0.4 kg) and mean BCS (7.6 vs. 6.0) decreased (P < 0.05) from wk 0 to wk 18. The NRC (2006) maintenance energy requirement (MER) for overweight cats is 130(BWkg0.40). In comparison, the mean MER during baseline in our study was 113(BWkg0.40). Throughout wk 1-4, 5-8, and 9-18, the energy levels to sustain weight loss were 76, 64, and 57% of baseline MER, respectively, demonstrating how restrictive feeding must be for consistent weight loss. Mean fat mass was decreased (P < 0.001) at wk 8, 12, and 16 (2417 , 2097 , and 1810 g, respectively) versus wk 0 (2924 g); therefore, body fat percentage also was decreased (P < 0.05) at wk 8, 12, and 16 (36.8, 34.0, 30.7%, respectively) versus wk 0 (40.9%). Mean lean body mass was lower (P < 0.01) at wk 12 and 16 (3671 and 3664 g, respectively) versus wk 0 (3865 g). Importantly, lean body mass percentage was increased (P < 0.05) at wk 8, 12, and 16 (61.5, 64.4, and 67.6%) versus wk 0 (57.6%). Mean daily activity tended to be higher (P = 0.061) at wk 12 vs. wk 0. The mean light:dark ratio of activity was increased ( P <0.05) at wk 18 vs wk 0, 6, and 12. Except for elevated mean creatinine (P < 0.05) during weight loss, all blood metabolites remained within reference ranges. Mean triglyceride concentrations were decreased (P < 0.05) throughout the weight loss phase. Using non-targeted gas chromatography, liquid chromatography, and mass spectrometry, a total of 535 named biochemicals were identified, with up to 269 metabolites altered (P- and q- values < 0.05) at any time point. Principal component analysis showed a continual shift in metabolite profile as weight loss progressed. Components 1 and 2 explained 14.3% and 10.3% of the variability, respectively. There was a significant and dramatic reduction of bile acids (cholate; taurocholate; deoxycholate) with weight loss. A reduction in numerous non-esterfied fatty acids (NEFA) and an increase in ketones (acetoacetate; 3-hydroxybutryate) and monoglycerides suggested a shift toward lipolysis and hepatic NEFA oxidation. Decreased markers of inflammation and oxidative stress were indicated by reduced pro-inflammatory oxylipids, eicosanoids, and oxidized biomarkers following weight loss. Mevalonate was decreased (P < 0.05) after wk 8 compared to baseline, which agrees with the reduced bile acids without altering cholesterol concentration. In conclusion, restricted feeding of a moderate-protein, high-fiber diet is a safe and effective means for weight loss in cats, leading to increased physical activity and reduced blood triglycerides. Global metabolomics identified biomarkers of reduced food intake, weight loss, and/or altered metabolism. Based on our data, the current NRC (2006) MER estimates for cats appear to be too high and should be reconsidered.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-08-01The student, Marissa Pallotto, accepted the attached license on 2015-06-16 at 12:12.The student, Marissa Pallotto, submitted this Thesis for approval on 2015-06-16 at 12:25.This Thesis was approved for publication on 2015-06-16 at 15:32.DSpace SAF Submission Ingestion Package generated from Vireo submission #8283 on 2015-09-29 at 15:05:15Made available in DSpace on 2015-09-29T21:02:51Z (GMT). No. of bitstreams: 3 PALLOTTO-THESIS-2015.pdf: 1666651 bytes, checksum: 33970fbf3589459bb8c748a3395908b6 (MD5) MS Thesis_6.16.docx: 990769 bytes, checksum: 6a09dede685746e513abda7910ac3ed2 (MD5) LICENSE.txt: 4213 bytes, checksum: fe5b2938aa35e0596c83e27ce3cfccd3 (MD5) Previous issue date: 2015-06-16Embargo set by: Seth Robbins for item 89529 Lift date: 2017-09-29T21:03:28Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 89529 Lift date: 2017-09-29T21:08:35Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemLimited Restriction Lifted for Item 89529 on 2017-09-30T09:15:32Z

    Ceftobiprole perspective: Current and potential future indications

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    Ceftobiprole combines an excellent spectrum for community-acquired pneumonia (CAP) and hospital-acquired pneumonia (HAP) pathogens, with a low/medium MDR risk, and the β-lactams’ safety in frail patients admitted to the hospital in internal medicine wards which may be at high risk of adverse events by anti-MRSA coverage as oxazolidinones or glycopeptides. We aimed to report the available evidence regarding ceftobiprole use in pneumonia and invasive bacterial infections, shedding light on ceftobiprole stewardship. The clinical application and real-life experiences of using ceftobiprole for bloodstream infections, including infective endocarditis, are limited but nevertheless promising. In addition, extended-spectrum ceftobiprole activity, including Entero-coccus faecalis, Enterobacteriaceae, and Pseudomonas aeruginosa, has theoretical advantages for use as empirical therapy in bacteremia potentially caused by a broad spectrum of microorganisms, such as catheter-related bacteremia. In the future, the desirable approach to sepsis and severe infections will be administered to patients according to their clinical situation, the intrinsic host characteristics, the susceptibility profile, and local epidemiology, while the “universal antibiotic strategy” will no longer be adequate

    Fibre-reinforced composite crowns luted to implant abutments via electroformed primary copings: an in vitro retention study.

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    Aim: The aim of this study was to investigate the retention values and mode of failure of metal crowns and fibre-reinforced composite crowns. Methods: Seventy-five implants and the corresponding abutments were used. After the galvanic copings were fabricated, the specimens were distributed into three groups: in group A traditional metal crowns were realized, whereas in groups B and C fibre-reinforced composite crowns were made. In group B the same resin based cement used in group A was employed for luting the fibre-reinforced composite crowns to the galvanic copings, while in group C a dedicated self-adhesive composite cement was used. All galvanic coping/crown assemblies were cemented onto abutments with Panavia 21 cement. Specimens were subjected to a pull-out test; median retentive values and standard deviations were calculated. Results: All samples in the group A showed separation at the abutment-galvanic cap interface. All samples in the group B showed decementation at galvanic cap-crown interface. In 13 samples of the group C the decementation occurred at the abutment-galvanic cap interface and in 12 samples of this group the decementation occurred at the galvanic cap-crown interface. The mean retention value was 659.1 N±162.9 for group A, 304.7 N±101.4 for B and 635.4 N±155.9 for C. Differences between groups A/B and B/C are statistically significant (t-student test, P<0.01), while is not statistically different between groups A/C. Conclusion: When a self-adhesive composite cement is used, fibre-reinforced composite single crowns and conventional metal crowns show similar retentive performance

    Breakthrough pneumonia, meningitis and bloodstream infection due to Streptococcus pneumoniae during cefixime therapy

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    Streptococcus pneumoniae is the main pathogen in invasive, life-threatening diseases such as bacteremia, meningitis, and pneumonia. We describe three cases of breakthrough pneumococcal severe life-threatening infections, including two meningitis and one bloodstream infection in patients treated with cefixime for otitis, sinusitis and pneumonia, respectively. Cefixime does not seem to be fully effective in treating invasive pneumococcal diseases. Because penicillin non-susceptibility might be linked to cefixime failure, the prompt knowledge of susceptibility to penicillin in S. pneumoniae might be very useful. Furthermore, MIC of cefixime should be measured because values >0.5 mg/L might be related to failure

    Under a Starry Vault. Warburg, Jung and the Renaissance of Ancient Paganisms at the Beginning of the 20<sup>th</sup> Century

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    The paper tackles the controversial question of the affinities between the work of Aby Warburg and Carl Gustav Jung. Instead of focussing her interest exclusively on the concepts of collective memory and primordial images, though, the author critically compares the different ways Warburg and Jung looked at the renaissance of ancient practices of Paganism at the beginning of the Twentieth century, and questions the extent to which the cultural crisis heralded by Modernity, and the challenges brought about by secularisation influenced their reading of the revivals.</p
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