1,720,974 research outputs found
Glucagon, a trophic hormone for the neonatal rat hepatocyte.
No full textGlucagon is a trophic hormone for the neonatal rat hepatocyte
Studies on hepatotrophic hormones: The role of glucagon.
No full textStudies on hepatotrophic hormones: The trophic role of glucagon
La dimensione metodologica e riflessiva nei processi di apprendimento dall'esperienza
No full textIl testo, composto da vari autori, rappresenta un tentativo di inquadrare i frameworks teorici che caratterizzano la formazione esperienziale. La prima parte è carattaerizata da contributi di accademici, la seconda da formatori esperienziali. Il volume rappresenta uno sforzo da parte di tutti autori di far chiarezza e di condividere, dubbi, criticità e punti di forza della formazione esperienziale
Studi sulle interazioni tra la colchicina e l’epatocita di Mammifero. I. Effetti della temperatura ambientale sulla incorporazione di [3H]colchicina da parte di cellule epatiche di Ratto in coltura primaria
No full textStudi sulle interazioni tra la colchicina e l’epatocita di Mammifero. I. Effetti della temperatura ambientale sulla incorporazione di [3H]colchicina da parte di cellule epatiche di Ratto in coltura primari
Neonatal rat hepatocytes in primary tissue culture free from density-dependent regulation of growth
No full textStudies employing [3H]thymidine and radioautography as well as colchicine and Feulgen staining of DNA showed that up to 19-fold increases in the degree of cell crowding in vitro, i.e. from 1.45 to 27.55 X 10(4) cells per specimen, did not change the rates of entry into DNA synthesis and mitosis of cultivated primary neonatal rat hepatocytes
Lack of synergism between epidermal growth factor and combined pancreatic hormones in stimulating in vitro neonatal hepatocyte growth
No full textLack of synergism between epidermal growth factor and combined pancreatic hormones in stimulating in vitro neonatal hepatocyte growt
Insulina e glucagone stimolano in modo sinergico la sintesi di nuovo DNA e l’ attività proliferativa degli epatociti di Ratto neonato in coltura primaria
No full textInsulina e glucagone stimolano in modo sinergico la sintesi di nuovo DNA e l’ attività proliferativa degli epatociti di Ratto neonato in coltura primari
The stimulation by epidermal growth factor (urogastrone) of the growth of neonatal rat hepatocytes in primary tissue culture and its modulation by serum and associated pancreatic hormones
No full textEpidermal growth factor (EGF) added in a single dose (between 10(-16) and 1.7 X 10(-9)M) to neonatal rat hepatocytes in primary culture with subsequent incubation for 12 and 24 hours in Eagle's MEM fortified with 10% (v/v) FBS stimulated their entry into S and M phases, as shown by (3H)thymidine labeling and autoradiography and by a 4-hour exposure to colchicine (0.1 mM). Growth stimulation by EGF was detectable after 4 hours, peaking between 12 and 16 hours, and thereafter declining in intensity. Rat hepatocytes exposed for 72 hours (between the fourth and the seventh day in vitro) to no serum or to 10% fresh FBS possessed similar growth rates and absolute numbers in the cultures. A 24-hour exposure to 20 to 50% FBS stimulated hepatocytic DNA synthesis and mitotic activity and resulted (except for the 50% FBS treatment) in increased hepatocytes' numbers, which were relatively greater than the concurrent increases in connective tissue cell numbers. In serum-devoid medium EGF (10(-11)M) enhanced hepatocytic mitotic, but not DNA-synthetic activity. To be fully effective EGF required a 10% FBS addition to the medium, then eliciting within 24 hours a marked increase in hepatocytes' number with respect to cultures incubated with 10% serum only. When associated with 20 to 30% FBS, EGF stimulated parenchymal cell growth at rates slightly higher, but not significantly different, than those elicited by the same serum concentrations alone. However, when used in conjunction with 10 to 30% FBS, EGF preferentially increased the number of hepatocytes rather than that of non-parenchymal cells. Moreover, comparative proliferation kinetic studies showed that in the presence of 10% FBS, an equimolar (10(-14)M) mixture of EGF, insulin, and glucagon promoted an early and marked increase in the DNA-synthetic and mitotic activities of hepatocytes, which peaked after 8 hours. Within a 24-hour time lag this growth stimulation was as effective in increasing the final hepatocytes' number as was a 1000-fold higher EGF concentration, and was twice as active as either an equimolar (10(-14)M) mixture of the two pancreatic hormones or EGF by itself at 10(-14)M. These results show that the growth-promoting effect of EGF on primary neonatal rat hepatocytes is modulated by serum factor(s) and can be additively amplified by the simultaneous administration of subphysiological doses of glucagon and insulin
Dialogare con le immagini. L’uso delle immagini nella ricerca e nella formazione esperienziale
No full textIl contributo evidenzia l'uso di immagini e disegni nella ricerca in educazione e formazione, all’interno di tecniche di animazione
o formazione di gruppi o di tecniche di intervista per l’appunto mediate da immagini e metafore e presenta due specifiche strategie:
• il Photolangage di Babin, Baptiste e Bélisle (1978), tecnica di animazione e formazione di gruppi mediata dall’uso di fotografie;
• l’Intervista epistemico-operativa messa a punto da Fabbri e Munari nell’ambito dell’Epistemologia Operativa (Fabbri, 1994; Fabbri e Munari,
2005), mediata da rappresentazioni metaforiche e/o simboliche.
Il contributo ha inoltre l’obiettivo di evidenziare come l’introduzione di strumenti di mediazione possa contribuire nel fare di una sessione formativa
o di un’intervista, un’attività esperienziale
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