69 research outputs found

    Awake! A Southern War Song

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    80.7568.107 – Awake! A Southern War Song: Words by J. M. Kilgour and Music by C. L. Peticolas: 1863: Vocal Solo

    Harp of the South, awake! and strike the strain once more [first line of chorus]

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    strophic with choruspiano and voiceJohns Hopkins University, Levy Sheet Music Collection, Box 093, Item 080Words by J.M. Kilgour. Music by C.L. Peticolas

    Harp of the South, awake! and strike the strain once more [first line of chorus]

    No full text
    strophic with choruspiano and voiceJohns Hopkins University, Levy Sheet Music Collection, Box 093, Item 080Words by J.M. Kilgour. Music by C.L. Peticolas

    Absence of Evidence for Metabolite-Modulated Association between α-Glycerol-3-phosphate Dehydrogenase and l-Lactate Dehydrogenase<sup>†</sup>

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    Evidence for the NADH-modulated formation of a complex between α-glycerol-3-phosphate dehydrogenase and l-lactate dehydrogenase was reported [Yong, H., Thomas, G. A., and Peticolas, W. L. (1993) Biochemistry 32, 11124−11131]. This NADH-modulated association suggested a mechanism of potentially great importance to enzyme modulation and the controversial phenomena of direct NADH channeling. In the present paper, we reproduce with additional controls the experiments described by Yong et al. ((1993) Biochemistry 32, 11124−11131). Our results conclusively demonstrate the absence of detectable association between α-glycol-3-phosphate dehydrogenase and l-lactate dehydrogenase

    The Raman Spectroscopy of Nucleic Acids

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    High resolution measurements and modeling of auroral hydrogen emission line profiles

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    Measurements in the visible wavelength range at high spectral resolution (1.3 A° ) have been made at Longyearbyen, Svalbard (15.8 E,78.2 N) during an interval of intense proton precipitation. The shape and Doppler shift of hydrogen Balmer beta line profiles have been compared with model line profiles, using as input ion energy spectra from almost coincident passes of the FAST and DMSP spacecraft. The comparison shows that the simulation contains the important physical processes that produce the profiles, and confirms that measured changes in the shape and peak wavelength of the hydrogen profiles are the result of changing energy input. This combination of high resolution measurements with modeling provides a method of estimating the incoming energy and changes in flux of precipitating protons over Svalbard, for given energy and pitch-angle distributions. Whereas for electron precipitation, information on the incident particles is derived from brightness and brightness ratios which require at least two spectral windows, for proton precipitation the Doppler profile of resulting hydrogen emission is directly related to the energy and energy flux of the incident energetic protons and can be used to gather information about the source region. As well as the expected Doppler shift to shorter wavelengths, the measured profiles have a significant red-shifted component, the result of upward flowing emitting hydrogen atoms

    Laser Raman Light-Scattering Observations of Conformational Changes in Myosin Induced by Inorganic Salts

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    The Raman spectra of aqueous solutions of myosin and mixtures of myosin in solutions of the salts CaCl2, MgCl2, and LiBr have been taken. The spectrum of the solvent background has been subtracted by means of a computer, leaving only the Raman peaks of the protein. From an analysis of the Raman bands in the regions at 900, 940, 1,240–1,300, and 1,650–1,670cm-1, it seems likely that CaCl2 effects an α-to β-transition in myosin, probably owing to the interaction of the Ca2+ ion, LiBr appears to denature the protein leading to increased random coil structure, and MgCl2 appears to have an effect intermediate between the two other salts. These results are reported for concentrations as low as 10-5 M of CaCl2 and MgCl2.This investigation indicates the usefulness of the Raman light-scattering technique for the study of protein conformational changes
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