1,068 research outputs found
Sister chromatid exchanges (SCE) in the cattle popolation Agerolese
No full textThe sister chromatid exchange (SCE) test has been used to check genome (DNA) stability of humans (Chaganti et al., 1974) and the main livestock species (Di Meo et al., 2000; Iannuzzi et al., 1990; Lopez and Arruga, 1992), as well as to discover DNA damage caused by a range of natural and artificially synthesised chemical compounds. The SCE test can
be performed during DNA replication, after two cell-cycle–replications in the presence of the thymidine analogue 5-bromodeoxyuridine (BrdU). While the sister chromatid with a native polynucleotidic chain (containing thymidine) is stained, the other sister chromatid, with BrdU in both polynucleotidic chains, is not stained. This allows easy visualization of the presence of SCEs. High number of SCEs is evidence for high genome instability and occurrence of possible mutations. The SCE test has also been used to determine the frequency of exchange on the active and inactive X chromosome in bovids (Iannuzzi et al., 1990) to compare genome stability of three different cattle breeds reared under similar conditions (significant differences in SCE mean values were found between Friesians— with lower numbers of SCE—and the Podolians) (Iannuzzi et al., 1991); and to compare the mean SCE values of animals with a normal karyotype and those (with significant higher SCE/cell mean values) carrying rob (1;29) (Rangel-Figueiredo et al., 1995). The aim of this work was to apply the SCE test on the Agerolese cattle population so as to check the genome stability of this endangered breed
Exalphus confusus Restello, Iannuzzi & Marinoni 2001
No full text718. Exalphus confusus Restello, Iannuzzi & Marinoni, 2001: 298, fig. 14 Holotype male: Brazil, Bahia: Encruzilhada (Motel da Divisa, Estr. Rio-Bahia, km 965, 960 m), XI. 1972, C.A.C. Seabra & O. Roppa.Published as part of Monné, Miguel A. & Monné, Marcela L., 2016, Checklist of Cerambycidae (Coleoptera) primary types of the Museu Nacional, Rio de Janeiro, Brazil, with a brief history of the collection, pp. 1-90 in Zootaxa 4110 (1) on page 54, DOI: 10.11646/zootaxa.4110.1.1, http://zenodo.org/record/25531
Successful pregnancy after uterine artery embolization for uterine arterovenous malformation: a rare case report.
No full textM Guida,1 D Maffucci,1 G Iannuzzi,1 M Giordano,1 G Luciano,1 L Di Benedetto,2 R Cantarella,3 A Rescigno,3 L Giugliano1 1Università degli studi di Salerno – Dipartimento di Medicina, Chirurgia e Odontoiatria “Scuola Medica Salernitana”, Salerno, Italy; 2Università degli studi di Roma “La Sapienza” – Dipartimento di Scienze Medico-Chirurgiche e di Medicina Traslazionale – Facoltà di Medicina e Psicologia Azienda Ospedaliera Sant’ Andrea, Rome, Italy; 3Azienda Ospedaliera Universitaria San Giovanni di Dio e Ruggi D’Aragona, Salerno, Italy Objective: This paper reports on a rare case of pregnancy after uterine artery embolization (UAE) for uterine arteriovenous malformation (AVM). Debate exists about persistence of fertility in women after UAE. Adverse effects of this technique can modify both uterine echostructure, inducing necrosis and infarction, endometrial atrophy and uterine artery rupture, and ovarian reserve, causing persistent amenorrhea. Ovarian reserve appears to be affected by UAE in premenopausal women. However, younger ovaries (according to biological ovarian age) exhibit a greater capacity for recovery after ovarian damage. Therefore, larger studies are needed for more conclusive results. Case report: A 28-year-old woman was admitted to our department due to life-threatening uterine bleeding, resulting in tachycardia, pallor, and sweating. The patient came with a history of two spontaneous miscarriages. After sonography and computed tomography, AVMs were identified at uterine fundus and anterior wall. Conclusion: The pathogenesis of infertility after UAE is not yet known. The peculiarity of this case was that, only few months later, the patient became pregnant and gave birth to a live fetus at 37 weeks with cesarean delivery. Keywords: uterine artery embolization, fertility, uterine arteriovenous malformation, pregnancy, angiograph
Werner Sombart and the global society. Anticipations from a classic author of sociology
No full textThe article examines the most salient works in which the sociologist Werner Sombart saw the dimension of globality as a social element, constitutive of mo- dernity and its future. The aim is to understand the role of globality, as an idea and a phenomenon, in the constitution in all aspects of society (economic, political, cultural, anthropological). The Works to which the article refers show a global society, one that goes from the time of Sombart to the present day, as the author had already envisioned in all their potentialities and criticalities
Chromosome Abnormalities and Fertility in Domestic Bovids: A Review
No full textAfter discovering the Robertsonian translocation rob(1;29) in Swedish red cattle and demonstrating its harmful effect on fertility, the cytogenetics applied to domestic animals have been widely expanded in many laboratories in order to find relationships between chromosome abnormalities and their phenotypic effects on animal production. Numerical abnormalities involving autosomes have been rarely reported, as they present abnormal animal phenotypes quickly eliminated by breeders. In contrast, numerical sex chromosome abnormalities and structural chromosome anomalies have been more frequently detected in domestic bovids because they are often not phenotypically visible to breeders. For this reason, these chromosome abnormalities, without a cytogenetic control, escape selection, with subsequent harmful effects on fertility, especially in female carriers. Chromosome abnormalities can also be easily spread through the offspring, especially when using artificial insemination. The advent of chromosome banding and FISH-mapping techniques with specific molecular markers (or chromosome-painting probes) has led to the development of powerful tools for cytogeneticists in their daily work. With these tools, they can identify the chromosomes involved in abnormalities, even when the banding pattern resolution is low (as has been the case in many published papers, especially in the past). Indeed, clinical cytogenetics remains an essential step in the genetic improvement of livestock
Premessa
No full textPresentazione del volume e cenni storici ai contenuti e all'interesse del carteggio tra Clara Maffei e Carlo Tenca, due tra i maggiori protagonisti della letteratura italiana in età risorgimentale
Molecular Cytogenetics in Domestic Bovids: A Review
No full textThe discovery of the Robertsonian translocation (rob) involving cattle chromosomes 1 and 29 and the demonstration of its deleterious effects on fertility focused the interest of many scientific groups on using chromosome banding techniques to reveal chromosome abnormalities and verify their effects on fertility in domestic animals. At the same time, comparative banding studies among various species of domestic or wild animals were found useful for delineating chromosome evolution among species. The advent of molecular cytogenetics, particularly the use of fluorescence in situ hybridization (FISH), has allowed a deeper investigation of the chromosomes of domestic animals through: (a) the physical mapping of specific DNA sequences on chromosome regions; (b) the use of specific chromosome markers for the identification of the chromosomes or chromosome regions involved in chromosome abnormalities, especially when poor banding patterns are produced; (c) better anchoring of radiation hybrid and genetic maps to specific chromosome regions; (d) better comparisons of related and unrelated species by comparative FISH mapping and/or Zoo-FISH techniques; (e) the study of meiotic segregation, especially by sperm-FISH, in some chromosome abnormalities; (f) better demonstration of conserved or lost DNA sequences in chromosome abnormalities; (g) the use of informatic and genomic reconstructions, in addition to CGH arrays, to predict conserved or lost chromosome regions in related species; and (h) the study of some chromosome abnormalities and genomic stability using PCR applications. This review summarizes the most important applications of molecular cytogenetics in domestic bovids, with an emphasis on FISH mapping applications
Comparison of genome stability in two pig breeds by using the sister chromatid exchange (SCE) test
No full textThe sister chromatid exchange (SCE) test has been used to detect genome stability in humans (Chaganti, 1974) and the main livestock species (Ciotola et al., 2004; Di Meo et al., 2000; Di Berardino et al., 1979), and to discover DNA damage caused by a variety of natural and artificial chemical compounds (Iannuzzi et al., 1990)
Exalphus spilonotus Restello, Iannuzzi & Marinoni 2001
No full text<p> <b> 721. <i>Exalphus spilonotus</i></b> Restello, Iannuzzi & Marinoni, 2001: 298, fig. 13</p> <p>Holotype male: Brazil, Pará: Jacarecanga, VI.1969, F.R. Barbosa.</p>Published as part of <i>Monné, Miguel A. & Monné, Marcela L., 2016, Checklist of Cerambycidae (Coleoptera) primary types of the Museu Nacional, Rio de Janeiro, Brazil, with a brief history of the collection, pp. 1-90 in Zootaxa 4110 (1)</i> on page 54, DOI: 10.11646/zootaxa.4110.1.1, <a href="http://zenodo.org/record/255316">http://zenodo.org/record/255316</a>
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