1,721,052 research outputs found
Effect of lycopene on fowl sperm characteristics during in vitro storage
No full textChicken and turkey sperm contains high amounts of PUFAs and spontaneous peroxidation occurs during in vitro storage. For this reason, the addition of antioxidant to diluent takes special interest in the improvement of commercial extenders. The effects of lycopene added to fowl sperm was the object of this study. Semen of 13 roosters, from 36th to 43rd week of age, was collected and pooled. Fresh semen was evaluated at 0 h time and 4 aliquots of 500μL were diluted to 2×109 sperm/mL by diluent Lake or Lake and lycopene at different concentrations (500μg/mL, 250μg/mL and 100μg/mL) and stored at 13/14°C in a water bath for 24 h. Motility, forward progressive motility and viability were evaluated at 1 h, 6 h and 24 h. Sperm motility and forward progressive motility were not significantly (P > 0.05) affected by lycopene inclusion. For 500μg/mL treatment the viability parameter was significantly (P < 0.05) higher than for Lake or Lake with 250μg/mL and higher for Lake with 100μg/mL. Besides, among the treatments the 500μg/mL of lycopene reduced the viability loss between 6 h and 24 h of incubation. The results of the present study suggest positive effects of lycopene supplementation to extender on fowl sperm survival during liquid storage
CRYOPRESERVATION OF POULTRY SEMEN - A REVIEW
No full textAlthough still in the experimental phase, the technique of freezing domestic fowl semen may prove of interest and value to the industry. Various stages of the application of this method of preservation still need to be improved. One of the most critical aspects is the choice of cryoprotectant and its use during the process of freezing and thawing. While glycerol has a better cryopreservative action than other cryoprotectants in domestic fowl semen, it exerts a widely demonstrated contraceptive action, the mechanism of which has not yet been clarified. Thus the use of glycerol as a cryoprotectant must be accompanied by its complete removal before insemination.
The fertilizing capacity of semen preserved by freezing is notably less than that of fresh (non-preserved) material and has been evaluated as 1.6% and 19.7%. Genetic influences appear to affect spermatozoan response and tolerance to thermal treatments resulting in differences in subsequent fertility. Most studies using fowl semen report final freezing temperatures between -79-degrees-C and -196-degrees-C, a cooling rate of 1-10-degrees-C/minute and a thawing rate of 50-70-degrees-C/minute.
Straws have been found to be more satisfactory containers than glass vials or ampoules for preserving semen from domestic fowl
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