132 research outputs found
Relazioni tra l' edera comune ( Hedera Helix L. ) E le piante ospiti in un ostrio-querceto Veneto (Tregnano-VR).
No full textQuesta tesi ha lo scopo di verificare le relazioni tra l' edera comune ( Hedera Helix L.) e le specie ospiti presenti in un ostrio-quercet Veneto in un bosco di Tregnago-VR
Archaeometric study of shells of Helicidae of the Edera Cave (North-Eastern Italy)
No full textThe Edera Cave, near Aurisina in the Trieste Karst, retains a stratigraphy that extends from
the Mesolithic to the modern era. At Sauveterrian layers, many specimens of Helix cincta
are present, which are considerably crushed, and a small percentage of which are also
blackened. Chemical analyses show that the blackened specimens were exposed to a
temperature of between 500 and 550°C, and suggest that many others were destroyed by fire
at temperatures of above 700°C. Consequently, only a part of the Helix cincta shells is
assumed to be the residue of human meals, since several factors render plausible an
accidental combustion of shells already present in the ground before the lighting of
Mesolithic hearth
MONITORING DATA DURING AND AFTER PREGNANCY TO EARLY DETECT RARE DISEASES: THE EDERA PROJECT
No full textRare diseases (RDs) are an important public health problem and an ongoing challenge for the medical community and health policy.
Because of the problems associated with access to care and information, people with RDs are very vulnerable at psychological/social/cultural level. Particular difficulties concern pregnant women affected with RDs or subjects with RDs that want to plan a pregnancy. In this context the project EDERA (Early DEtection of Rare diseAses) was designed and funded by the Agenzia Regionale per la Salute ed il
Sociale (AReSS Puglia), and includes the collaboration of the group of Databases and Information Systems of the Department of Engineering for Innovation of the University of Salento with four Patient’s associations (HHT Onlus, CIDP Italia Onlus, APMAR Onlus and ANACC Onlus).
The overall objective of the project is the knowledge in the field of maternal, fetal and child health, monitoring for the prevention, surveillance and early detection of RDs during and after pregnancy. Specifically, the project aims at creating a digital system for collecting data on maternal, fetal and child health by means of innovative techniques that exploit smartphones as a complementary, if not alternative, way to common methods for data collection. Patients will be able to easily provide data related to lifestyle (nutrition, physical activity, drugs, etc.), pregnancy (symptoms, treatments, moods, etc.), delivery (type, complications, etc.) and children features (weight, motor function, stimulus response, etc.). Data willbe shared respecting the rules of privacy and treatment of sensitive data, through accurate access management techniques. The approval of the project is a good starting result and shows the high interest of the medical community and health policy on these issues.
The system will be tested and validated in collaboration with physicians, associations and patients considering a comprehensive approach that takes into account the heterogeneity of clinical characteristics and lifestyles of participants. The network will include not only the proposing four Patient’s associations but also other external partners interested in the initiative that will be involved in the awareness campaign, with the participation of medical specialists (gynecologists, obstetricians, pediatrists, neurologists, neurosurgeons, etc.) of several Hospitals throughout Puglia region that will contribute to present the initiative to the patients
Towards a comprehensive picture of C-to-U RNA editing sites in angiosperm mitochondria
No full textKey message: Our understanding of the dynamic and evolution of RNA editing in angiosperms is in part limited by the few editing sites identified to date. This study identified 10,217 editing sites from 17 diverse angiosperms. Our analyses confirmed the universality of certain features of RNA editing, and offer new evidence behind the loss of editing sites in angiosperms. Abstract: RNA editing is a post-transcriptional process that substitutes cytidines (C) for uridines (U) in organellar transcripts of angiosperms. These substitutions mostly take place in mitochondrial messenger RNAs at specific positions called editing sites. By means of publicly available RNA-seq data, this study identified 10,217 editing sites in mitochondrial protein-coding genes of 17 diverse angiosperms. Even though other types of mismatches were also identified, we did not find evidence of non-canonical editing processes. The results showed an uneven distribution of editing sites among species, genes, and codon positions. The analyses revealed that editing sites were conserved across angiosperms but there were some species-specific sites. Non-synonymous editing sites were particularly highly conserved (~ 80%) across the plant species and were efficiently edited (80% editing extent). In contrast, editing sites at third codon positions were poorly conserved (~ 30%) and only partially edited (~ 40% editing extent). We found that the loss of editing sites along angiosperm evolution is mainly occurring by replacing editing sites with thymidines, instead of a degradation of the editing recognition motif around editing sites. Consecutive and highly conserved editing sites had been replaced by thymidines as result of retroprocessing, by which edited transcripts are reverse transcribed to cDNA and then integrated into the genome by homologous recombination. This phenomenon was more pronounced in eudicots, and in the gene cox1. These results suggest that retroprocessing is a widespread driving force underlying the loss of editing sites in angiosperm mitochondria.Fil: Edera, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Gandini, Carolina Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentin
Efficacy and safety of 0.5% levobupivacaine versus 0.5% bupivacaine for peribulbar anesthesia
No full textElena Pacella,1 Fernanda Pacella,1 Fabiana Troisi,2 Domenico Dell'Edera,3 Paolo Tuchetti,4 Tommaso Lenzi,1 Saul Collini21Department of Sense Organs, Faculty of Medicine and Dentistry, Rome, 2Department of Medical Surgical Sciences and Translational Medicine Sapienza, Faculty of Medicine and Psychology, Roma, University of Rome, Rome, 3Unit of Cytogenetic and Molecular Genetics, Madonna delle Grazie Hospital, Matera, 4National Institute for Health, Migration and Poverty, Rome, ItalyBackground: This randomized double-blind study examined the use of a new anesthetic agent, levobupivacaine 0.5%, which is the S(−)-enantiomer of a racemic mixture of bupivacaine, for peribulbar anesthesia and compared it with racemic bupivacaine 0.5% alone or in combination with hyaluronidase 10 IU/mL.Methods: A total of 160 patients undergoing ophthalmic surgery were randomized into four groups (n = 40 each) to receive inferotemporal peribulbar injection of levobupivacaine 0.5% (group L), racemic bupivacaine 0.5% (group B), levobupivacaine + hyaluronidase 10 IU/mL (group LH), or racemic bupivacaine + hyaluronidase 10 IU/mL (group BH) by two anesthetists and two ophthalmologists in a ratio of 25% each. Ocular akinesia and orbicularis oculi function were evaluated using a three-point scale; a value < 5 points was considered as requiring surgery, and movements were re-evaluated the day following surgery to confirm regression of the block.Results: The time to onset (12 ± 2.6 minutes versus 13 ± 2.8 minutes) and duration of anesthesia (185 ± 33.2 minutes versus 188 ± 35.7 minutes) were similar between groups L and B. Complete akinesia (score 0) was obtained more frequently when hyaluronidase was used in addition to the anesthetic, with occurrences of 72.5% versus 57.5% in group LH versus L, respectively, and 67.5% versus 45% in group BH versus B. Moderate hypotension (<30% of baseline) was observed in four patients (10%) in group L, two (5.0%) in group B, one (2.5%) in group LH, and three (7.5%) in group BH. The time to onset was significantly different between groups L and BH, B and BH, and LH and BH, and the duration of anesthesia differed significantly between groups B and LH, B and BH, and L and LH. The akinesia score differed significantly between groups L and LH and between groups B and LH (P = 0.043 and P = 0.018, respectively), and the number of patients with a score of 0 differed significantly between groups B and LH and between groups B and BH (P = 0.004 and P = 0.017, respectively).Conclusion: Levobupivacaine is a long-lasting local anesthetic with limited cardiotoxicity and neurotoxicity, and may be considered the landmark for vitreoretinal surgery in elderly patients.Keywords: bupivacaine, levobupivacaine, ophthalmic surgery, peribulbar block, regional anesthesi
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Study of the doubly and singly Cabibbo suppressed decays D+ --> K+ pi- pi+ and D(s)+ --> K+ pi- pi+ in the FOCUS experiment
No full textThis thesis illustrates a complete study of the doubly and singly Cabibbo suppressed decays D{sup +} and D{sub s}{sup +} {yields} K{sup +} {pi}{sup -}{pi}{sup +}. Data for this analysis have been collected by the fixed-target high-energy photoproduction experiment FOCUS at Fermilab. The authors have selected the D{sup +} and D{sub s}{sup +} samples with cuts to obtain a sufficiently high statistics, a good signal to noise ratio and, at the same time, eliminate possible contaminations from the more copious and favored decays. The D{sup +} yield consists of 189 {+-} 24 events, with a signal to noise ratio {approx} 1; the D{sub s}{sup +} yield is 567 {+-} 31 and the signal to noise ratio is {approx} 2.5. The authors have measured {Lambda}(D{sup +} {yields} K{sup +}{pi}{sup -}{pi}{sup +})/{Lambda}(D{sup +} {yields} K{sup -}{pi}{sup +}{pi}{sup +}) = 0.0065 {+-} 0.0008 {+-} 0.004 and {Lambda}(D{sub s}{sup +} {yields} K{sup +}{pi}{sup -}{pi}{sup +})/{Lambda}(D{sub s}{sup +} {yields} K{sup +}K{sup -}{pi}{sup +}) = 0.127 {+-} 0.007 {+-} 0.014, improving the previous determinations of a factor of 2 and 5, respectively. The author has also performed a Dalitz plot analysis for both decays. The amplitude analysis for D{sub s}{sup +} {yields} K{sup +}{pi}{sup -}{pi}{sup +} represents the first available measurement for this channel
Secondary structure prediction of long noncoding RNA: review and experimental comparison of existing approaches
No full textMOTIVATION:
In contrast to messenger RNAs, the function of the wide range of existing long noncoding RNAs (lncRNAs) largely depends on their structure, which determines interactions with partner molecules. Thus, the determination or prediction of the secondary structure of lncRNAs is critical to uncover their function. Classical approaches for predicting RNA secondary structure have been based on dynamic programming and thermodynamic calculations. In the last 4 years, a growing number of machine learning (ML)-based models, including deep learning (DL), have achieved breakthrough performance in structure prediction of biomolecules such as proteins and have outperformed classical methods in short transcripts folding. Nevertheless, the accurate prediction for lncRNA still remains far from being effectively solved. Notably, the myriad of new proposals has not been systematically and experimentally evaluated.
RESULTS:
In this work, we compare the performance of the classical methods as well as the most recently proposed approaches for secondary structure prediction of RNA sequences using a unified and consistent experimental setup. We use the publicly available structural profiles for 3023 yeast RNA sequences, and a novel benchmark of well-characterized lncRNA structures from different species. Moreover, we propose a novel metric to assess the predictive performance of methods, exclusively based on the chemical probing data commonly used for profiling RNA structures, avoiding any potential bias incorporated by computational predictions when using dot-bracket references. Our results provide a comprehensive comparative assessment of existing methodologies, and a novel and public benchmark resource to aid in the development and comparison of future approaches.This work was supported by ANPCyT (PICT 2018 3384, PICT 2018 2905, PICT 2019 3420) and UNL (CAI+D 2020 115). Researchers from sinc(i)
and IAL are collaborating in the framework of the Program Science and Technology against Hunger, supported by the Argentinian Ministry of Science, to study and develop ncRNAs as exogenous bioactive molecules in agriculture. UC was funded by MICINN (IJC2019-039402-I). The
work used computational resources from the Pirayu cluster, acquired with funds from the Santa Fe Science, Technology and Innovation Agency
(ASACTEI), Project AC-00010-18, Res. No. 117/14. This equipment is part of the National High Performance Computing System of the Ministry of Science and Technology of Argentina. We also acknowledged the support of NVIDIA Corporation for the donation of GPUs used for this
researchPeer Reviewed"Article signat per 12 autors/es: L A Bugnon, A A Edera, S Prochetto, M Gerard, J Raad, E Fenoy, M Rubiolo, U Chorostecki, T Gabaldón, F Ariel, L E Di Persia, D H Milone, G Stegmayer"Postprint (author's final draft
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