1,355 research outputs found
ANTIOXIDANT AND ANTIMUTAGENIC ACTIVITY OF CANNABIS SATIVA L. SEEDS AND SPROUTS
No full textNo available abstrac
Paulus Frassinetti, Ed. of Atellanae Fabulae (Poetarvm Latinorvm Reliqviae, P. L. R. Aetas rei publicae, vol. VI. I), 1967
No full textTaladoire Barthélémy A. Paulus Frassinetti, Ed. of Atellanae Fabulae (Poetarvm Latinorvm Reliqviae, P. L. R. Aetas rei publicae, vol. VI. I), 1967. In: Revue des Études Anciennes. Tome 70, 1968, n°3-4. p. 485
La nuova disciplina sulle notificazioni a mezzo posta
No full textL'autrice analizza le modifiche apportate dalla legge di bilancio 2018 ( l. n. 205/2017) alla disciplina delle notificazioni a mezzo posta degli atti giudiziari, per adeguarla al processo di liberalizzazione dei servizi postali, iniziato con la legge annuale per il mercato e la concorrenza n. 124/2017. L'autrice rileva che, sebbene le norme siano state riformate con il dichiarato fine di assicurare, sia effettivi risparmi di spesa, sia l'efficienza del servizio a tutela dell'amministrazione della giustizia e della finanza pubblica, vi è stata tuttavia poca chiarezza nella loro formulazione, da cui conseguono diversi dubbi interpretativi
Le condizioni di procedibilità dell'azione di risarcimento danni da responsabilità sanitaria
No full textL'articolo illustra le differenze tra la consulenza tecnica preventiva, ex art. 696 bis, e la mediazione, strumenti entrambi a cui è condizionato il giudizio di responsabilità medica, la cui scelta spetta alla parte istante. Vengono inoltre proposte possibili soluzioni alle problematiche legate al fatto che il legislatore nell'art. 8 della l. n. 24 del 2017 contempla solo l'ipotesi in cui la condizione di procedibilità della domanda viene assolta con la consulenza tecnica ex art. 696 bis c.p.c., legando ad essa, in caso di esito negativo, l'instaurazione del giudizio di merito unicamente con il procedimento semplificato di cognizione
Commento all'art. 709 c.p.c.
No full textL'autore analizza la nuova formulazione dell'art. 709 c.p.c, così come risulta dalle modifiche ad esso apportate dalla l. n. 80 del 200
Genotoxicity of 4-nonylphenol and nonylphenol ethoxylate mixtures with the use of Saccharomyces cerevisiae D7 mutation assay and use of this text to evaluate the efficiency of biodegradation treatments.
No full textNonylphenol ethoxylates (NPnEOs, n is the number of ethoxylic units in the molecule) are non-ionic surfactants widely used in several industrial applications, such as textile and leather processing, paper industry, formulation of pesticides, paints and washing cleaners. 4-Nonylphenol (4-NP), the main product of NPnEO biodegradation, is a toxic xenobiotic compound classified as endocrine disrupter. While numerous studies reported the toxicity and estrogenic activity of nonylphenols, little is known about the mutagenicity of these compounds. In this paper, the genotoxicity of 4-NP and NPnEO mixtures was evaluated by using the D7 strain of Saccharomyces cerevisiae as experimental model. The same tests were applied to effluents deriving from laboratory scale packed-bed bioreactors, developed for the treatment of NPnEO contaminated wastewater, in order to evaluate the residual genotoxic potential with respect to the influent waste. The target compounds fed to the bioreactors were 4-NP and NPnEO mixtures possessing an average of 5 or 1.5 ethoxylic units (Igepal CO-520 and Igepal CO-210, respectively). The results showed that 4-NP induced significant cytotoxic effect on S. cerevisiae cells at 60 mg/L, as well as mutagenic effects at 15 and 30 mg/L. 4-NP was the most genotoxic compound among those assayed, followed by Igepal CO-210, whereas Igepal CO-520 did not induce genotoxicity at any of the assayed concentrations. The genotoxic effects evidenced on yeast cells treated with 4-NP disappeared after the treatment in the bioreactor. This indicates that the biological treatment is capable of removing not only the pollutant, but also the toxicity associated to the compound and its degradation metabolites. This study represents, to the best of our knowledge, the first report that evaluates the genotoxicity of both 4-NP, NPnEOs and their potential aerobic degradation products on an eukaryotic organism. The obtained results suggest that the S. cerevisiae D7 strain is a very effective model to study the induction of genotoxic damage by the compounds under study. In addition, this method is much more simpler and easier to be applied than other tests described in the literature to study genotoxic effects. Moreover, the test described in this work has also proven to be effective in evaluating the toxicity of effluents deriving from laboratory scale biotreatment processes. It would be really interesting to apply the described tests on real wastewater treatment system in order to estimate the detoxification potential of the process applied, which is often not directly correlated with the removal of the target pollutant
Antimicrobial and antibiofilm activity against Staphylococcus aureus of Opuntia ficus-indica (L.) mill. cladode polyphenolic extracts
No full textPlant extracts are a rich source of natural compounds with antimicrobial properties, which are able to prevent, at some extent, the growth of foodborne pathogens. The aim of this study was to investigate the potential of polyphenolic extracts from cladodes of Opuntia ficus-indica (L.) Mill. to inhibit the growth of some enterobacteria and the biofilm formation by Staphylococcus aureus. Opuntia ficus-indica cladodes at two stages of development were analysed for total phenolic content and antioxidant activity by Oxygen Radical Absorbance Capacity (ORAC) and Trolox equivalent antioxidant capacity (TEAC) (in vitro assays) and by cellular antioxidant activity in red blood cells (CAA-RBC) (ex vivo assay). The Liquid Chromatography Time-of-Flight Mass Spectrometry (LC/MS-TOF) analysis of the polyphenolic extracts revealed high levels of piscidic acid, eucomic acid, isorhamnetin derivatives and rutin, particularly in the immature cladode extracts. Opuntia cladodes extracts showed a remarkable antioxidant activity (in vitro and ex vivo), a selective inhibition of the growth of Gram-positive bacteria, and an inhibition of Staphylococcus aureus biofilm formation. Our results suggest and confirm that Opuntia ficus-indica cladode extracts could be employed as functional food, due to the high polyphenolic content and antioxidant capacity, and used as natural additive for food process control and food safety
Arbuscular mycorrhizal fungi affect total phenolics content and antioxidant activity in leaves of oak leaf lettuce varieties
No full textPlant secondary metabolites are considered key bioactive compounds for a healthy diet. Arbuscular mycorrhizal fungi (AMF) may interact with host plant metabolism, inducing the accumulation of health-promoting phytochemicals and antioxidant molecules. Lettuce is a largely consumed vegetable, which may interact with AMF to alter its content of secondary metabolites and natural antioxidants molecules, as previously shown in cultivars belonging to var. capitata or var. longifolia. In this study, the effects of red and green leaf Lactuca sativa var. crispa inoculation with different AMF species, Rhizoglomus irregulare and Funneliformis mosseae, were investigated, by assessing the total phenolics and anthocyanins content, and the antioxidant activity of leaf tissue. A significant increase of antioxidant activity and of phenolics were observed in plants of both cultivars inoculated with R. irregulare, compared to non inoculated plants. Likewise, anthocyanins (in red leaf lettuce) were more abundant in inoculated plants than in controls. Altogether, the results indicate that R. irregulare strain showed a stronger ability than F. mosseae in affecting plant metabolism and that mycorrhizal inoculation may be used to enhance concentration of phenolics in leaf type lettuces, provided that a suitable AMF is selected
Antimutagenic and Antioxidant Activity of a Selected Lectin-free Common Bean (Phaseolus vulgaris L.) in Two Cell-based Models
No full textLegumes and particularly beans are a key food of Mediterranean diet representing an important source of proteins, fiber, some minerals and vitamins and bioactive compounds. We evaluated the antioxidant and anti-mutagenic effects of a new fermented powder of a selected lectin-free and phaseolamin-enriched variety of common bean (Phaseolus vulgaris L.), named Lady Joy. Lady Joy lysate (Lys LJ) was studied in human erythrocytes and in Saccharomyces cerevisiae yeast cells. The antioxidant and anti-hemolytic properties of Lys LJ, studied in an ex vivo erythrocytes system using the cellular antioxidant assay (CAA-RBC) and the hemolysis test, evidenced a dose-dependent antioxidant activity as well as a significant hemolysis inhibition. Besides, results evidenced that Lys LJ treatment significantly decreased the intracellular ROS concentration and mutagenesis induced by hydrogen peroxide in S. cerevisiae D7 strain. In conclusion, Lys LJ showed both an antimutagenic effect in yeast and a strong scavenging activity in yeast and human cells
Genotoxicity of 4-nonylphenol and nonylphenol ethoxylate mixtures by the use of Saccharomyces cerevisiae D7 mutation assay and use of this text to evaluate the efficiency of biodegradation treatments.
No full textNonylphenol ethoxylates (NPnEOs, where n is the number of ethoxylic units in the molecule) are non-ionic surfactants widely used for domestic and industrial purposes. 4-Nonylphenol (4-NP), the main product of NPnEO biodegradation, is a toxic xenobiotic compound classified as endocrine disrupter. While numerous studies reported the toxicity and estrogenic activity of nonylphenols, little is known about the mutagenicity of these compounds. In this paper, the genotoxicity of 4-NP and NPnEO mixtures was evaluated by using the D7 strain of Saccharomyces cerevisiae as experimental model. The same genotoxicity tests were applied to effluents deriving from experimental packed-bed bioreactors, developed for the treatment of NPnEO contaminated wastewater, in order to evaluate the residual genotoxic potential with respect to the influent waste. The target compounds fed to the bioreactors were 4-NP and NPnEO mixtures possessing an average of 5 or 1.5 ethoxylic units (Igepal CO-520 and Igepal CO-210, respectively). The results showed that 4-NP induced significant cytotoxic effect on S. cerevisiae cells at 50 mg/L, as well as mutagenic effects at the lowest tested concentrations (12 and 25 mg/L). 4-NP was the most genotoxic compound among those assayed, followed by Igepal CO-210, whereas Igepal CO-520 did not induce genotoxicity at any of the assayed concentrations. The genotoxic effects of 4-NP on yeast cells disappeared after the treatment of 4-NP artificially contaminated water in the bioreactor. This indicates that the biological treatment is capable of removing not only the pollutant, but also the toxicity associated to the compound and its degradation metabolites. This study represents, to the best of our knowledge, the first report that evaluates the genotoxicity of both 4-NP, NPnEOs and their potential aerobic degradation products on an eukaryotic organism. The obtained results suggest that the S. cerevisiae D7 strain is a very effective model microorganism to study the induction of genotoxic damage by the compounds under study. Moreover this yeast assay has been proved effective to evaluate the detoxification effect deriving from biotreatment processes
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