8,600 research outputs found
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
Full text linkThe interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification
The biomarkers of human papillomavirus infection in tonsillar squamous cell carcinoma-molecular basis and predicting favorable outcome.
No full textCarbazole–oxadiazole containing polyurethanes as phosphorescent host for organic light emitting diodes
No full textThe highly specific platelet glycoprotein (GP) VI agonist trowaglerix impaired collagen-induced platelet aggregation ex vivo through matrix metalloproteinase-dependent GPVI shedding.
No full text
Stress disturbances caused by the inhomogeneity in an elastic half-space subjected to contact loading
No full text[[abstract]]Inhomogeneities can increase localized stress and cause microstructural alterations to initiate fatigue failures in rolling elements under cyclic contact loading. To study the stress disturbances created by the inhomogeneity, a two-dimensional contact stress analysis is presented for a cylindrical indenter sliding on an elastic half-space containing an inhomogeneity of arbitrary shape. The boundary element method is used to analyze the contact problem, where actual contact boundary, contact pressure as well as tractions and displacements at inhomogeneity-substrate interface are determined by solving a set of integral equations numerically. Numerical results are presented to investigate effects and the stress disturbances caused by the inhomogeneity with various locations, sizes and material properties of inhomogeneity. The results also show that hard inclusions are more detrimental than soft deformable particles in rolling contact elements. (c) 2006 Elsevier Ltd. All rights reserved.[[note]]SC
Contact stress analysis of an elastic half-plane containing multiple inclusions
No full text[[abstract]]This paper presents a two-dimensional contact stress analysis to investigate the effects of multiple inclusions on the contact pressure and subsurface stresses in an elastic half-plane. The boundary element method is used to analyze the contact problem where a set of integral equations is derived on the contact region and the matrix- inclusion interfaces. As the contact region is unknown a priori, an iterative procedure is implemented to determine the actual contact region and the contact pressure, and the tractions and displacements on the matrix-inclusion interfaces are obtained by solving the integral equations numerically. Numerical results show that the inclusions near contact surface could cause significant alterations in the contact pressure distribution. The stiff inclusions could toughen the surrounding material and reduce the internal stresses while the soft inclusions could increase the subsurface stresses. (c) 2008 Elsevier Ltd. All rights reserved.[[note]]SC
Fabrication and characterization of high-T-c YBa2Cu3O7-x nanoSQUIDs made by focused ion beam milling
No full textNovobiocin sensitizes BCRP/MXR/ABCP overexpressing topotecan-resistant human breast carcinoma cells to topotecan and mitoxantrone.
No full text- …
