1,169 research outputs found
Abstract 3611: Proteasome subunit PSMD1 participates in p53 degradation and regulates proliferation of breast cancer cells
No full textAbstract
Breast cancer is the most common cancer among women in developed countries. Generally, 70% of primary breast cancer patients express estrogen receptor, thus endocrine therapy is often efficient for the disease using antiestrogen such as tamoxifen as well as aromatase inhibitor. During long-term therapy, however, a substantial proportion of women recurrent and acquire resistance to endocrine therapy. Understanding of the mechanism underlying acquired endocrine resistance would be a first step toward overcoming hormone-refractory cancer. In this study, we identified candidate genes that contributed to tamoxifen resistance in MCF-7 breast cancer cells by functional screening based on short hairpin RNA (shRNA) library. We found that several candidate genes were overexpressed in breast cancer patients and their abundance was positively associated with poor prognosis of cancer patients in clinical database. Moreover, the genes associated with poor prognosis also showed positive association with shorter survival for patients treated with tamoxifen. Expression levels of these candidate genes were upregulated in hydroxytamoxifen (OHT)-resistant MCF-7 cells (OHTR) compared with parental cells. Proliferation of OHTR cells was repressed by siRNAs targeting 26S subunits, non-ATPase 1 (PSMD1) and tetraspanin12 (TSPAN12). Furthermore, knockdown of PSMD1 inhibited cell cycle progression and p53 protein degradation. p21 and 14-3-3σ, target genes of p53, were upregulated by PSMD1 knockdown. These findings would be used for the development of alternative diagnostic and therapeutic options for endocrine-resistant breast cancers.
Citation Format: Toshiyuki Okumura, Kazuhiro Ikeda, Wataru Sato, Kuniko Horie-Inoue, Satoshi Inoue, Satoru Takeda, Koji Okamoto. Proteasome subunit PSMD1 participates in p53 degradation and regulates proliferation of breast cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 3611. doi:10.1158/1538-7445.AM2017-3611</jats:p
Prognostic phenotypic and genotypic factors associated with photodynamic therapy response in patients with age-related macular degeneration
No full textTakashi Tsuchihashi,1 Keisuke Mori,1 Kuniko Horie-Inoue,2 Yasushi Okazaki,3 Takuya Awata,4,5 Satoshi Inoue,2 Shin Yoneya1 1Department of Ophthalmology, 2Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA; 3Division of Translational Research, Research Center for Genomic Medicine, 4Division of Endocrinology and Diabetes, Department of Medicine, 5Division of RI Laboratory, Biomedical Research Center, Saitama Medical University, Iruma, Saitama, Japan Background: This study aimed to demonstrate the phenotypic and genotypic factors associated with photodynamic therapy (PDT) for age-related macular degeneration (AMD).Methods: The study included 149 patients with exudative AMD treated by PDT. Eight phenotypic factors and ten genotypic factors for three single nucleotide polymorphisms (SNPs; rs800292, rs1061170, rs1410996) in the complement factor H (CFH) gene, rs 11200638-SNP in the high temperature requirement A-1 (HTRA1) gene, two SNPs (rs699947, rs2010963) in the vascular endothelial growth factor (VEGF) gene, and four SNPs (rs12948385, rs12150053, rs9913583, rs1136287) in the pigment epithelium-derived factor (PEDF) gene were evaluated. Results: A significant association with best-corrected visual acuity change was demonstrated in the greatest linear dimension, presence or absence of pigment epithelial detachment, and HTRA1-rs11200638 genotype statistically (P=3.67×10-4, 1.95×10-2, 1.24×10-3, respectively). Best-corrected visual acuity in patients with AA genotype of HTRA1-rs11200638 significantly decreased compared with that in patients with GG genotype (P=1.33×10-3). Logistic regression analyses demonstrated HTRA1-rs11200638 genotype was most strongly associated with best-corrected visual acuity outcome from baseline at 12 months after photodynamic therapy (P=4.60×10-3; odds ratio 2.363; 95% confidence interval 1.303–4.285).Conclusion: The HTRA1-rs11200638 variant showed the most significant association. Therefore, this variant may be used as a prognostic factor to estimate the PDT response with significant predictive power. Keywords: age-related macular degeneration, photodynamic therapy, phenotypic and genotypic factors, high temperature requirement A-1, greatest linear dimension, pigment epithelial detachmen
Agalope liuzihaoi Huang, Horie, Fan, Wang & Espeland, 2023, sp. n.
No full text<i>Agalope liuzihaoi</i> S.-Y. Huang & Horie sp. n. <p>(Figs 14, 54, 73)</p> <p> <b>Type material.</b> <b>Holotype:</b> male, hindwritten label (in Chinese) “ 2017. 8. 13, LZH, <b>AENJffiṄDZ</b> ”(2017. 8. 13 (13. VIII. 2017), Bomi, Zhagang Town (Gang Village, Zhamu Town, Bomi County, Xizang Autonomous Region), LZH (leg. Zi-hao Liu))/ hindwritten label“ZSY183” (CHSY, will be subsequently deposited in ZFMK).</p> <p> <b>Diagnosis.</b> Length of forewing 21 mm in male. Externally <i>A. liuzihaoi</i> <b>sp. n.</b> is only reminiscent of <i>A. geoffi</i> <b>sp. n.</b> described above, but it can be distinguished from the latter by the combination of the following characters: 1) Both wings broader. 2) On the hindwing the cilia are white thoroughly from apex to tornus, while in <i>A. geoffi</i> <b>sp. n.</b> the cilia are greyish from apex to cell Cu 2 and white from cell Cu 2 to the tornus. 3) The posterior tegumenal projection is slightly longer. 4) The distal saccular process is significantly long and slender, while in <i>A. geoffi</i> <b>sp. n.</b> it is much shorter and forms a triangular process pointed upwards. 5) The phallus is somewhat longer.</p> <p> <b>Distribution.</b> Known from Bomi County in southeastern Xizang, China, its type locality.</p> <p> <b>Etymology.</b> The specific epithet <i>liuzihaoi</i> is dedicated to the collector of the new species, Dr. Zi-hao Liu, a good friend of the first author. A noun in the genitive case.</p>Published as part of <i>Huang, Si-Yao, Horie, Kiyoshi, Fan, Xiao-Ling, Wang, Min & Espeland, Marianne, 2023, A review of the genus Agalope Walker (Lepidoptera, Zygaenidae, Chalcosiinae) with taxonomic notes and descriptions of three new genera and three new species, pp. 291-321 in Zootaxa 5284 (2)</i> on page 297, DOI: 10.11646/zootaxa.5284.2.4, <a href="http://zenodo.org/record/7923410">http://zenodo.org/record/7923410</a>
Capacitors
No full textThis webpage is part of a larger site by the author about electronic circuit engineering. This page introduces the reader to capacitors, including the principles behind how they work. Explanations of several different types of capacitors (including Electrolytic, Tantalum, and Mica) are accompanied by detailed color illustrations of the concepts
Analysis of mitochondrial respiratory chain complexes by blue native electrophoresis: Focusing on a supercomplex assembly-promoting factor, COX7RP
No full textIdentification of estrogen-responsive genes based on the DNA binding properties of estrogen receptors using high-throughput sequencing technology
No full textLecideoid lichens of Prince Olav Coast and Soya Coast, Enderby Land, East Antarctica
No full textFive species of lecideoid lichens belonging to three genera are reported as new to the flora of the Prince Olav Coast and the Soya Coast, Enderby Land, East Antarctica; they are Carbonea capsulata (DODGE & BAKER) HALE, Lecidea andersonii R. FILSON, Lecidea cancriformis DODGE & BAKER, Lecidea soyaensis M. INOUE and Lecidella siplei (DODGE & BAKER) M. INOUE. All are endemic to Antarctica. An examination was made of the collections from the region, which were collected mainly by the author under the project of the 27th Japanese Antarctic Research Expedition (JARE-27,during January 1986 and February 1987). The type and authentic specimens of possibly related species have been restudied. Lecidea soyaensis M. INOUE is new to science and Lecidella siplei (DODGE & BAKER) M. INOUE is proposed as a new combination. Taxonomic and chemical data as well as a key to the known taxa are provided
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