112 research outputs found

    Comparison of Safety and Efficacy of Intrastromal Injections of Voriconazole, Amphotericin B and Natamycin in Cases of Recalcitrant Fungal Keratitis: A Randomized Controlled Trial

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    Gunjan Saluja,1 Namrata Sharma,1 Rinky Agarwal,1 H P Sharma,2 Deepali Singhal,1 Prafulla Kumar Maharana,1 Rajesh Sinha,1 Tushar Agarwal,1 T Velpandian,2 Jeewan S Titiyal,1 Gita Satpathy3 1Department of Ophthalmology, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India; 2Ocular Pharmacology and Pharmacy Division, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India; 3Department of Ocular Microbiology, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, IndiaCorrespondence: Namrata SharmaDepartment of Ophthalmology, Cornea, Cataract and Refractive Surgery Services, 4th Floor Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, Ansari Nagar East, New Delhi, Tel +91-11-26593144Fax +91-11-26588919Email [email protected]: To compare the safety and efficacy of intrastromal voriconazole (IS-VCZ), amphotericin B (IS-AMB) and natamycin (IS-NTM) as an adjunct to topical natamycin (NTM) in cases of recalcitrant fungal keratitis.Design: Prospective randomized trial.Setting: Tertiary eye centre.Participants: Sixty eyes of 60 patients with microbiologically proven recalcitrant fungal keratitis (ulcer size > 2 mm, depth > 50% of stroma, and not responding to topical NTM therapy for two weeks) were recruited.Methods: patients were randomized into three groups of 20 eyes, each receiving ISVCZ 50ug/0.1 mL, ISAMB, 5ug/0.1 mL and ISNTM 10ug/0.1 mL (prepared aseptically in ocular pharmacology). The patients in all three groups continued topical NTM 5% every four hours until the ulcer healed. Primary outcome measure was time taken till complete clinical resolution of infection, and secondary outcome measure was best corrected visual acuity (BCVA) at six months.Results: All three groups had comparable baseline parameters. The mean duration of healing was significantly better (p=0.02) in the ISNTM group (34± 5.2 days) as compared to the ISVCZ group (36.1± 4.8 days) and the ISAMB group (39.2± 7.2 days). About 95%, 90% and 95% patients healed successfully in the ISVCZ, ISAMB and ISNTM groups, respectively. In terms of healing, deep vascularization was significantly greater in the ISAMB group (55%, p=0.02) when compared to the ISVCZ and ISNTM groups (31% and 26%, respectively). There were fewer repeat injections in the ISNTM group (7/20 vs 8/20 and 9/20 in the ISVCZ and ISNTM groups, respectively).Conclusion: Intrastromal injections are a safe and effective adjunct to conventional therapy in the management of recalcitrant fungal keratitis. ISNTM had a similar visual outcome with faster healing while ISAMB had a higher rate of deep vascularization after healing.Keywords: intrastromal injections, recalcitrant fungal keratiti

    Prospective Role of Plant Growth Regulators for Tolerance to Abiotic Stresses

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    Abiotic stresses (heat, drought, salinity, and heavy metals) are projected to drastically reduce crop yields leading to seriously jeopardizing the food security of increasing population across the globe. Plant growth regulators (PGRs) are natural or synthetic substances that regulate the growth and differentiation of plant cells, tissues, and organs. Although the roles of PGRs in improving plant growth and development are well documented, their role in plant stress tolerance is continued to be explored. Among the PGRs, auxins, gibberellins, cytokinins, abscisic acid, jasmonic acid, ethylene, salicylic acid, nitric oxide, brassinosteroids, strigolactones, and polyamines have got the attention of the researchers as a sustainable source to improve abiotic stress tolerance under changing climate. Recently, numerous advancements have led to identify PGRs effects and to understand how plant growth responses are regulated by PGRs under abiotic stress. Different strategies are being employed to improve plant growth under abiotic conditions; the application of PGRs in crops offers good outcomes. However, it needs more researches and discussions on the said subject. Therefore, this chapter is focused on the stress management and role of PGRs in conferring abiotic stress tolerance under changing climate as it will play a key role to policymakers’ food, nutritional, environmental, and economic security for the present and future generation demand.Fil: Sabagh, Ayman E. L.. University Of Kafrelsheikh; EgiptoFil: Hossain, Akbar. Bangladesh Wheat And Maize Research Institute; BangladeshFil: Sohidul Islam, Mohammad. Hajee Mohammad Danesh Science And Technology University; BangladeshFil: Aamir Iqbal, Muhammad. University Of Poonch Rawalakot; PakistánFil: Amanet, Khizer. Comsats University Islamabad; PakistánFil: Mubeen, Muhammad. Comsats University Islamabad; PakistánFil: Nasim, Wajid. The Islamia University Of Bahawalpur (iub); PakistánFil: Wasaya, Allah. Bahauddin Zakariya University; PakistánFil: Llanes, Analia Susana. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Ratnasekera, Disna. University Of Ruhuna; Sri LankaFil: Kumar Singhal, Rajesh. Banaras Hindu University; IndiaFil: Kumari, Arpna. Guru Nanak Dev University; IndiaFil: Swaroop Meena, Ram. Banaras Hindu University; IndiaFil: Abdelhamid, Magdi. No especifíca;Fil: Hasanuzzaman, Mirza. Sher-e-Bangla Agricultural University; BangladeshFil: Ali Raza, Muhammad. Sichuan Agricultural University; ChinaFil: Özyazici, Gülen. Siirt University; TurquíaFil: Arif Ozyazici, Mehmet. Siirt University; TurquíaFil: Erman, Murat. Siirt University; Turquí
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