106 research outputs found
Study and Analysis of Patient Monitoring Compatibility with LabVIEW
No full textM.E.Current patient monitoring systems are based on embedded systems hardware and employs complex software algorithms. Large numbers of preventable deaths occur in hospitals each year, due to adverse events such as cardiac arrest and unplanned admission into Intensive Care Units (ICUs) from other hospital wards. The development of such patient monitoring systems is time consumption. Patient monitoring using LabVIEW has been considered for improving the quality of healthcare to an increased number of patients, including those suffering from physical and cognitive disabilities. For such patients, there is a need to design a comprehensive LabVIEW patient monitoring system.
Development of patient monitoring system in LabVIEW saves a lot of time using its interactive GUI interface. LabVIEW offers design and analysis all together in one package. The system can be tested along with the design phase for its reliability. The design files can then be embedded directly in the micro-controller for ease of development. In this work the study has been presented to compare of the patient monitoring system implementation in LabVIEW with its equivalent general approach to hardware implementation.EIE
Mutational bias in spermatogonia impacts the anatomy of regulatory sites in the human genome
No full textATAC-Seq reports local chromatin accessibility and provides a snapshot of active regulatory regions and genomic regions occupied by DNA-binding proteins in a given tissue. We used ATAC-Seq to identify open chromatin sites in FGFR3-positive spermatogonial cells isolated from dissociated human testicular samples. FGFR3 is most highly expressed in self-renewing spermatogonial stem cells, with low expression also being detected in early differentiating spermatogonia; its expression thus overlaps with the onset of PRDM9 expression in pre-meiotic spermatogonia. Open chromatin in FGFR3-positive cells was identified using standard peak detection analysis with MACS2 software.Talmane, Lana; Kaiser, Vera; Kumar, Yatendra; Semple, Fiona; MacLennan, Marie; Semple, Colin; Fitzpatrick, David; Taylor, Martin. (2021). Mutational bias in spermatogonia impacts the anatomy of regulatory sites in the human genome, [dataset]. University of Edinburgh. MRC IGC. MRC Human Genetics Unit. https://doi.org/10.7488/ds/3053
The role of the nuclear basket in regulating the senescence-associated secretory phenotype
Full text linkOncogene-induced senescence is a state of irreversible cell cycle arrest, accompanied by the secretion of signalling molecules, including pro-inflammatory cytokines and chemokines. These are collectively known as the senescence-associated secretory phenotype (SASP). The SASP enhances immune cell recruitment, aiding the clearance of senescent cells, but can also contribute to tumorigenesis. TPR, a protein in the nuclear basket of the nuclear pore complex, is known to be necessary for SASP induction in fibroblasts undergoing Ras-induced senescence.
In this thesis I investigate the mechanism by which TPR impacts the SASP. Using ATAC-seq, I show that potential enhancer elements, often close to key SASP genes, become active in senescent cells, but that this activity is lost when TPR is knocked down. I identify that these putative enhancers contain binding sites for NF-κB, a transcription factor known to regulate the SASP and I show that NF-κB activation is reduced in senescent cells without TPR.
Decreased NF-κB activation is already evident after three days of Ras activation and TPR knockdown, preceding induction of the SASP. RNA-seq carried out at the same timepoint shows transcriptional downregulation of STING upon TPR depletion. This is accompanied by reduced levels of cytoplasmic chromatin fragments (CCFs), which are known to activate NF-κB and the SASP through cGAS-STING signalling. This suggests that the nuclear pore complex may play a role in the blebbing off of the nuclear membrane to form CCFs in senescent cells.
I also investigate a potential role in senescence for the recently discovered nuclear basket protein ZC3HC1. I confirm that ZC3HC1 is present at the nuclear periphery and its localisation is dependent on TPR. However, contrary to previous reports, TPR localisation to nuclear pores does not depend on ZC3HC1. Unlike TPR, ZC3HC1 knockdown does not affect the SASP, and knockdown of ZC3HC1 causes different transcriptional changes from those that occur on TPR knockdown, suggesting distinct roles for the two nuclear basket proteins
A unique nucleosome arrangement, maintained actively by chromatin remodelers facilitates transcription of yeast tRNA genes
Full text linkBackground: RNA polymerase (pol) III transcribes a unique class of genes with intra-genic promoters and high transcriptional activity. The major contributors to the pol III transcriptome, tRNAs genes are found scattered on all chromosomes of yeast. A prototype tDNA of <150 bp length, is generally considered nucleosome-free while some pol III-transcribed genes have been shown to have nucleosome-positioning properties.
Results: Using high resolution ChIP-chip and ChIP-seq methods, we found several unique features associated with nucleosome profiles on all tRNA genes of budding yeast, not seen on nucleosome-dense counterparts in fission yeast and resting human CD4+ T cells. The nucleosome-free region (NFR) on all but three yeast tDNAs is found bordered by an upstream (US) nucleosome strongly positioned at −140 bp position and a downstream (DS) nucleosome at variable positions with respect to the gene terminator. Perturbation in this nucleosomal arrangement interferes with the tRNA production. Three different chromatin remodelers generate and maintain the NFR by targeting different gene regions. Isw1 localizes to the gene body and makes it nucleosome-depleted, Isw2 maintains periodicity in the upstream nucleosomal array, while RSC targets the downstream nucleosome. Direct communication of pol III with RSC serves as a stress-sensory mechanism for these genes. In its absence, the downstream nucleosome moves towards the gene terminator. Levels of tRNAs from different families are found to vary considerably as different pol III levels are seen even on isogenes within a family. Pol III levels show negative correlation with the nucleosome occupancies on different genes.
Conclusions: Budding yeast tRNA genes maintain an open chromatin structure, which is not due to sequence-directed nucleosome positioning or high transcription activity of genes. Unlike 5′ NFR on pol II-transcribed genes, the tDNA NFR, which facilitates tDNA transcription, results from action of chromatin remodeler Isw1, aided by Isw2 and RSC. The RSC-regulated nucleosome dynamics at the 3′ gene-end serves as a novel regulatory mechanism for pol III transcription in vivo, probably by controlling terminator-dependent facilitated recycling of pol III. Salient features of yeast tDNA chromatin structure reported in this study can explain the basis of the novel non-transcriptional roles ascribed to tDNAs
Tunable and switchable dual-band BPF with reconfigurable selectivity and bandwidth control
No full textmoney in the mental lives of the poor: Sample from indian urban working-class population
No full textThe project explores the psychological dimensions of poverty among the Indian working-class population, focusing on the impact of economic scarcity on thoughts related to money and financial stress. The literature review reveals how poverty influences the decision-making, risk behaviour, and cognitive functioning of the individuals dealing with it. The study aims to provide a nuanced understanding of the diverse thought patterns shaped by different material conditions within the poor population.
The methodology involves a conceptual replication of a study conducted by Shah et al. (2018), presenting two vignettes to participants representing real-life scenarios. Two scenarios include Study 1, “a visit to the doctor.”, and Study 2, “festival celebration.” After presenting one scenario per participant, they were asked to respond with three prominent thoughts that would arise if they were in that situation. We surveyed 240 participants in two categories: 1. those who get their wages on monthly wages and 2: those who get their wages on daily wages
RF-MEMS-Based Bandpass-to-Bandstop Switchable Single- and Dual-Band Filters With Variable FBW and Reconfigurable Selectivity
No full textmoney in the mental lives of the poor: Sample from indian urban working-class population
No full textThe project explores the psychological dimensions of poverty among the Indian working-class population, focusing on the impact of economic scarcity on thoughts related to money and financial stress. The literature review reveals how poverty influences the decision-making, risk behaviour, and cognitive functioning of the individuals dealing with it. The study aims to provide a nuanced understanding of the diverse thought patterns shaped by different material conditions within the poor population.
The methodology involves a conceptual replication of a study conducted by Shah et al. (2018), presenting two vignettes to participants representing real-life scenarios. Two scenarios include Study 1, “a visit to the doctor.”, and Study 2, “festival celebration.” After presenting one scenario per participant, they were asked to respond with three prominent thoughts that would arise if they were in that situation. We surveyed 240 participants in two categories: 1. those who get their wages on monthly wages and 2: those who get their wages on daily wages
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