1,253 research outputs found
MEF cells
No full textDate of collection wrong in the tilt series ID. Should be Jan 3, 2019<strong>Tilt Series Date:</strong> 2018-11-06</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> tomo3D</p>
<strong>Collaborators and Roles:</strong> Wei Yang froze cells, Danielle FIB milled and Shrawan/Danielle collected tilt series</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown on R2/2 gold quantifoil grids coated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> 3min 10uM FCCP, plunge frozen and FIB milled.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-39</p>TS13.mrc, Tilt Series (Pixel Size 0.432 nm), 1.9 GB
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TS13_bin4_SIRT_rotx.rec, Reconstruction (Pixel Size 0.0 nm), 741.2 MB
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MEF cells
No full textDate of collection wrong in the tilt series ID. Should be Jan 3, 2019<strong>Tilt Series Date:</strong> 2018-11-06</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> tomo3D</p>
<strong>Collaborators and Roles:</strong> Wei Yang froze cells, Danielle FIB milled and Shrawan/Danielle collected tilt series</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown on R2/2 gold quantifoil grids coated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> 3min 10uM FCCP, plunge frozen and FIB milled.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-32</p>TS6.mrc, Tilt Series (Pixel Size 0.432 nm), 1.6 GB
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TS6_bin4_SIRT_rotx.rec, Reconstruction (Pixel Size 0.0 nm), 1.0 GB
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MEF cells
No full textDate of collection wrong in the tilt series ID. Should be Jan 3, 2019<strong>Tilt Series Date:</strong> 2018-11-06</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> tomo3D</p>
<strong>Collaborators and Roles:</strong> Wei Yang froze cells, Danielle FIB milled and Shrawan/Danielle collected tilt series</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown on R2/2 gold quantifoil grids coated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> 3min 10uM FCCP, plunge frozen and FIB milled.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-28</p>TS2.mrc, Tilt Series (Pixel Size 0.432 nm), 1.7 GB
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TS2_bin4_SIRT_rotx.rec, Reconstruction (Pixel Size 0.0 nm), 833.9 MB
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MEF cells
No full textDate of collection wrong in the tilt series ID. Should be Jan 3, 2019<strong>Tilt Series Date:</strong> 2018-11-06</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> tomo3D</p>
<strong>Collaborators and Roles:</strong> Wei Yang froze cells, Danielle FIB milled and Shrawan/Danielle collected tilt series</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown on R2/2 gold quantifoil grids coated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> 3min 10uM FCCP, plunge frozen and FIB milled.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-33</p>TS7.mrc, Tilt Series (Pixel Size 0.432 nm), 1.7 GB
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TS7_bin4_SIRT_rotx.rec, Reconstruction (Pixel Size 0.0 nm), 997.8 MB
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MEF cells
No full textDate of collection wrong in the tilt series ID. Should be Jan 3, 2019<strong>Tilt Series Date:</strong> 2018-11-06</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> tomo3D</p>
<strong>Collaborators and Roles:</strong> Wei Yang froze cells, Danielle FIB milled and Shrawan/Danielle collected tilt series</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown on R2/2 gold quantifoil grids coated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> 3min 10uM FCCP, plunge frozen and FIB milled.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-35</p>TS9.mrc, Tilt Series (Pixel Size 0.432 nm), 1.6 GB
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TS9_bin4_SIRT_rotx.rec, Reconstruction (Pixel Size 0.0 nm), 641.4 MB
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MEF cells
No full textDate of collection wrong in the tilt series ID. Should be Jan 3, 2019<strong>Tilt Series Date:</strong> 2018-11-06</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> tomo3D</p>
<strong>Collaborators and Roles:</strong> Wei Yang froze cells, Danielle FIB milled and Shrawan/Danielle collected tilt series</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown on R2/2 gold quantifoil grids coated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> 3min 10uM FCCP, plunge frozen and FIB milled.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-42</p>TS16.mrc, Tilt Series (Pixel Size 0.432 nm), 1.8 GB
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TS16_bin4_SIRT_rotx.rec, Reconstruction (Pixel Size 0.0 nm), 890.9 MB
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MEF cells
No full textDate of collection wrong in the tilt series ID. Should be Jan 3, 2019<strong>Tilt Series Date:</strong> 2018-11-06</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Species / Specimen:</strong> MEF cells</p>
<strong>Strain:</strong> </p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 33000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> reupload_downloaded</p>
<strong>Processing Software Used:</strong> tomo3D</p>
<strong>Collaborators and Roles:</strong> Wei Yang froze cells, Danielle FIB milled and Shrawan/Danielle collected tilt series</p>
<strong>Purification / Growth Conditions / Treatment:</strong> grown on R2/2 gold quantifoil grids coated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> 3min 10uM FCCP, plunge frozen and FIB milled.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-11-06-29</p>TS3.mrc, Tilt Series (Pixel Size 0.432 nm), 1.5 GB
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TS3_bin4_SIRT_rotx.rec, Reconstruction (Pixel Size 0.0 nm), 1.0 GB
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Shrawan Kumar Trivedi's Quick Files
No full textThe Quick Files feature was discontinued and it’s files were migrated into this Project on March 11, 2022. The file URL’s will still resolve properly, and the Quick Files logs are available in the Project’s Recent Activity
Shrawan Kumar Trivedi's Quick Files
No full textThe Quick Files feature was discontinued and it’s files were migrated into this Project on March 11, 2022. The file URL’s will still resolve properly, and the Quick Files logs are available in the Project’s Recent Activity
HeLa (Vps4B KD) - PM damaged
No full textHeLa (Kyoto line stably expressing CHMP4B-EGFP at low levels; courtesy of MPI) knocked down for Vps4B (double transfection) and laser damaged. Grid - WY54_grid1.<strong>Tilt Series Date:</strong> 2018-07-08</p>
<strong>Data Taken By:</strong> Shrawan kumar Mageswaran</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x. </p>
<strong>Microscope:</strong> Caltech Titan Krios</p>
<strong>Acquisition Software:</strong> SerialEM</p>
<strong>Upload Method:</strong> pipeline</p>
<strong>Processing Software Used:</strong> IMOD, tomo3D</p>
<strong>Collaborators and Roles:</strong> HeLa cell line courtesy of Max Planck Institute (MPI). Wei Yang grew cells on grids, transfected with siRNA and caused plasma membrane damage using Laser before Shrawan froze the grids and performed CLEM and ECT.</p>
<strong>Purification / Growth Conditions / Treatment:</strong> Cells grown on R2/2 quantifoil gold grids treated with 0.1 mg/mL fibronectin.</p>
<strong>Sample Preparation:</strong> Cells were knocked down for Vps4B with SMART pool siRNA for 48 hr (2 x 24 hr). Laser - 640nm, 100%, 35 cycles, speed 7, Damaged area - 1.5umx1.5um. Cells were treated with 1.3uM AlPcS3a ~10 min before damage.</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2018-07-08-8</p>TS14.mrc, Tilt Series (Pixel Size 0.541 nm), 1.6 GB
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