170 research outputs found
Molecular Analysis of Salmonella Enterica Strains Carried by Poultry Entering the Food Chain in Trinidad
No full textThe present study was carried out with the aim to isolate and identify Salmonella spp. from poultry caecal samples collected from pluck shops in four zones of Trinidad. The study was also conducted to determine antimicrobial resistance of 91 isolates of Salmonella to 13 antimicrobial agents, virulence and resistance genes profiles and genetic relatedness of Salmonella serotypes by Pulsed Field Gel Electrophoresis (PFGE). A total of 1503 caecal samples of freshly slaughtered poultry were randomly collected from pluck shops across the country. The samples were screened for Salmonella by biochemical, serological tests and PCR was used for molecular characterization. Ninety-one (6.1%) of the 1503 samples were positive for Salmonella spp. The highest frequency of positivity for Salmonella spp. was recorded in North east zone (59.3%) of Trinidad. Ten different serotypes were detected from all Salmonella isolates. S. Molade (56.0%) was the predominant serotype identified. All 91 isolates of Salmonella spp. exhibited resistance to one of the 13 antimicrobial agents. The highest frequency of resistance was detected to Ampicillin (51.0%), followed by Kanamycin (49.5%) and Streptomycin (37.4%). Multiple drug resistance (MDR) was exhibited by 90.0% of Salmonella isolates. The frequency of detection of virulence genes in isolates of Salmonella ranged from 0.0% (viaB) to 100.0% for invA, mgtB, pipA and spi4D. PFGE profiles showed that Salmonella isolates were genetically diverse. A total of 20 PFGE groups were detected. The antibiograms of the isolates were clearly much more variable, which suggest that genotypic antimicrobial resistance may not relate to the phenotypic antibiograms in dendrograms except for qnrB gene. The findings provide evidence that poultry from pluck shops are colonized by pathogenic Salmonella harbouring antimicrobial resistance genes. It is evident that there is a need for prudent use of antimicrobial agents in poultry production systems and should be constantly monitored in Trinidad
Image-Guided Percutaneous Sclerotherapy for Orbital Low-Flow Malformation: Our Experience
No full textFor a safe sclerotherapy session to be completed in the orbital low-flow malformation (namely lymphovenous malformation or venolymphatic malformation), accurate identification of the target lesion for the drug injection is crucial. Regarding the dependability and viability of the injection approach, the authors have discussed their experiences with image-guided percutaneous sclerotherapy on a few patients
Cytogenetical Study of Induced Desynaptic Variants in <i>Phaseolus vulgaris</i> L.
No full textSecondary chromosomal association in kidney bean (Phaseolus vulgaris L.)
No full textAbstract The present study documents the mutagenic efficacy of gamma ray and sodium azide on the chromosomal association pattern of bivalents and meiotic behavior in Phaseolus vulgaris L. The seeds were irradiated with different doses of gamma rays viz.10 krad, 20 krad, and 30 krad from a 60 Co source and thereafter, the seeds were treated with 0.3% of freshly prepared sodium azide solution for three hours, respectively. The results clearly show the formation of various types of secondary chromosomal association among bivalents. Secondary association is defined as the tendency of bivalents to lie in pairs having diffused connections. The phenomenon of secondary pairing manifested from metaphase I stage and persisted upto metaphase II stage. The bivalents lie side by side and end to end to form secondary pairing. A secondary association between bivalents is considered to be of great significance as it is being taken as an indicator of ploidy in plants. Apart from secondary chromosomal association, other meiotic abnormalities were also noticed. These include precocious movement of chromosomes at metaphase I/II (2.83%), stickiness at metaphase I/II (3.45%), stickiness at anaphase I/II (2.20%), bridge (1.25%), unorientation (0.94%), micronuclei (1.88%) etc. This phenomenon, along with other meiotic aberrations affects the pollen fertility considerably
TLC AND HPLC Fingerprint development of Aegle marmelos Corr. And its polyherbal marketed formulations
No full textAbstract Chromatographic fingerprint analysis of herbal drugs represents a comprehensive qualitative approach for the purpose of species authentication, evaluation of quality and ensuring the consistency and stability of herbal drugs and their related products. In the present research article TLC and HPLC Chromatogram of Aegle marmelos Corr is taken as standard for comparing its fingerprinting profile with three marketed formulations containing Aegle marmelos Corr as one ingredient. Bilva patra showed four spots at 0.89, 0.75, 0.65, and 0.52. in thin layer chromatography. Bilwadi churna also showed three spots at 0.89 ,0.80 and 0.63. Pushyanug churna showed two spots at 0.89 and 0. .Whereas Gangadhar churna showed two spots of which only one spot is matching with Bilva Patra (0.63). On the inspection of various chromatogram of Bilva Patra and its formulations in Polyherbal drugs, gives various peaks from 4.8-10.. It seems that the peaks at 4.8,5.8,and 9.2 may be beneficial for standardizing the Bilva Patra in the polyherbal formulations,but when the chromatogram of polyherbal formulations have been been recorded , it was found that the peaks obtained at 9.2 have been retarded to a negligible amount, but only the peak of 5.8 min can be taken as standard
Stability and Bifurcation Analysis of Hassell–Varley Prey–Predator System with Fear Effect
No full text
Exploratory Study of the Companies’ Economic Performance Using a Social Customer Relationship Management
No full textAbstractThe epistemological positioning of scientific approach involves the diversity of the viewpoints of academics and specialists, exclusiveness not being considered. This paper aims to reflect own contributions to the development of management theory and management practice in Romania. First of all, the proposed scientific approach is a positivist approach, because it envisages the interposition of own reflections, an observation of what is happening in the market, an authentic presentation as it is. The main objective of this scientific research is to highlight the importance of social media as a mean of business development by creating new opportunities through promotion and a better brand visibility in the market. In the literature we have outlined a number of methods to assess the way in which companies approach the CRM: call method, the interview, the questionnaire, etc. Quantitative investigation of this paper was conducted based on assessment questionnaire, addressed to the employees of some Romanian companies. The qualitative investigation was conducted at the end of the research, based on analysis and structuring the information obtained. In this paper there were analyzed three elements: management position with the implementation of a CRM system; CRM to SCRM transition and the use of the new SM technologies in the implementation of a CRM system; applying of a SCRM system using social media platforms. As a result of the conducted study it was found that the media is one of the main trainers of public opinion, if not the main, controlling the public sphere. It conveys information, but also it controls and steers the public opinion. Thus, SM can form quickly enough media crisis to lower the public favorability for certain companies or politicians. In this way, by resorting to specific Social Media tools, the companies can obtain crucial information for evaluating the consumer satisfaction, information that would be more difficult to obtain traditionally
Effect of deposition time on the properties of Al doped ZnO films prepared by DC magnetron sputtering
No full textDesign and implementation of a simulation tool to study wait times in cataract surgery
No full textEye cataracts are a common problem for senior people. Long wait times for cataract surgery degrade the patients' quality of life. Reduction in long wait times in eye cataract surgery has got importance as one of the five major priority areas in the health care systems in Canada. The main contribution of this thesis is to design and develop a discrete event simulation tool in JAVA to study the wait times (wait time 1 and wait time 2) for patients in cataract surgical procedure. Two cataract surgical procedures are simulated in the simulation tool Northern Health Cataract Surgical Model (NHCS Model) and Cataract Surgery Generic Model (CSG Model). Two alternative patient referral methods (refer patients to the surgeon with the least number of patients and uniform distribution of patients) are proposed and compared to the existing method to examine which method results in reduced wait times. The impacts of changing the resources (surgeon and OR) on wait times were analysed. The Manitoba Cataract Waiting List Program (MCWLP) priority system is simulated and compared to the existing FCFS policy to see whether the scheduling of patients for surgery based on priority improves wait times. Experimental results show that the two proposed methods significantly reduce wait times. It is found that Northern Health would meet the target wait time 2 (16 weeks) if one more OR (total of two ORs) is allocated for cataract surgery. The use of priority scheduling did not show any improvement in wait time 2. Increasing budget or number of resources is not always easy for any health care authority. This thesis suggests that, if Northern Health authority changes the existing patient referral method, it would definitely reduce wait times for patients.The original print copy of this thesis may be available here: http://wizard.unbc.ca/record=b205527
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