94 research outputs found
Simulation-Guided 3D Nanomanufacturing <i>via</i> Focused Electron Beam Induced Deposition
Focused
electron beam induced deposition (FEBID) is one of the few techniques
that enables direct-write synthesis of free-standing 3D nanostructures.
While the fabrication of simple architectures such as vertical or
curving nanowires has been achieved by simple trial and error, processing
complex 3D structures is not tractable with this approach. In part,
this is due to the dynamic interplay between electron–solid
interactions and the transient spatial distribution of absorbed precursor
molecules on the solid surface. Here, we demonstrate the ability to
controllably deposit 3D lattice structures at the micro/nanoscale,
which have received recent interest owing to superior mechanical and
optical properties. A hybrid Monte Carlo–continuum simulation
is briefly overviewed, and subsequently FEBID experiments and simulations
are directly compared. Finally, a 3D computer-aided design (CAD) program
is introduced, which generates the beam parameters necessary for FEBID
by both simulation and experiment. Using this approach, we demonstrate
the fabrication of various 3D lattice structures using Pt-, Au-, and
W-based precursors
Additional file 4: of Transcriptome-enabled marker discovery and mapping of plastochron-related genes in Petunia spp.
Supplemental Dataset 3. Gene Ontology annotations of representative transcripts. (XLS 16720Â kb
Additional file 3: of Transcriptome-enabled marker discovery and mapping of plastochron-related genes in Petunia spp.
Supplemental Dataset 2. OrthoMCL clusters across three Petunia species. (XLSM 1433Â kb
Additional file 1: Figure S1. of Transcriptome-enabled marker discovery and mapping of plastochron-related genes in Petunia spp.
Work flow used for assemblies allowing incorporation of reads from all 5 tissue libraries while removing redundancy. Figure S2. Distributions of the number of isoforms for each transcript. Figure S3. SNP discovery pipeline with reads aligned to P. axillaris reference transcriptome assembly. Figure S4. Gene Ontology (GO) categories of the unigenes and unigenes with SNPs. Figure S5. Gene Ontology (GO) categories of the unigenes with more than ten SNPs. Distribution of the GO categories assigned to the unigenes were annotated in three categories: cellular components, molecular functions, and biological processes. Table S1. Primer sequences used for SNP validation by Sanger sequencing. Table S2. Primer sequences for CAPS markers developed from plastochron-related genes. (DOCX 782Â kb
Additional file 6: of Transcriptome-enabled marker discovery and mapping of plastochron-related genes in Petunia spp.
Supplemental Dataset 5. SSR primers derived from P. axillaris transcriptome. (XLSX 414Â kb
missing_busco_list_asm_v2.2_1000.tsv
List of missing Embryophyta orthologs from the genome assembl
cac_genome_assembly_v2.4.fa
Genome assembly of C. acuminata, containing 1,394 scaffold sequence
cal_FPKM_matrix.txt
Table with FPKM expression values for high-confidence genes in C. gigante
cac_hc_gene_models.repr.pep.fa
Protein sequences of representative models of high-confidence genes in C. acuminat
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