94 research outputs found

    Simulation-Guided 3D Nanomanufacturing <i>via</i> Focused Electron Beam Induced Deposition

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    Focused electron beam induced deposition (FEBID) is one of the few techniques that enables direct-write synthesis of free-standing 3D nanostructures. While the fabrication of simple architectures such as vertical or curving nanowires has been achieved by simple trial and error, processing complex 3D structures is not tractable with this approach. In part, this is due to the dynamic interplay between electron–solid interactions and the transient spatial distribution of absorbed precursor molecules on the solid surface. Here, we demonstrate the ability to controllably deposit 3D lattice structures at the micro/nanoscale, which have received recent interest owing to superior mechanical and optical properties. A hybrid Monte Carlo–continuum simulation is briefly overviewed, and subsequently FEBID experiments and simulations are directly compared. Finally, a 3D computer-aided design (CAD) program is introduced, which generates the beam parameters necessary for FEBID by both simulation and experiment. Using this approach, we demonstrate the fabrication of various 3D lattice structures using Pt-, Au-, and W-based precursors

    Additional file 1: Figure S1. of Transcriptome-enabled marker discovery and mapping of plastochron-related genes in Petunia spp.

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    Work flow used for assemblies allowing incorporation of reads from all 5 tissue libraries while removing redundancy. Figure S2. Distributions of the number of isoforms for each transcript. Figure S3. SNP discovery pipeline with reads aligned to P. axillaris reference transcriptome assembly. Figure S4. Gene Ontology (GO) categories of the unigenes and unigenes with SNPs. Figure S5. Gene Ontology (GO) categories of the unigenes with more than ten SNPs. Distribution of the GO categories assigned to the unigenes were annotated in three categories: cellular components, molecular functions, and biological processes. Table S1. Primer sequences used for SNP validation by Sanger sequencing. Table S2. Primer sequences for CAPS markers developed from plastochron-related genes. (DOCX 782 kb
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