5,189 research outputs found
Th2 and Th1 response to TM in Mina KO mice.
No full textShown are the percentage and absolute number of (A) CD4+IL4+ cells and (B) CD4+IFNγ+ cells and (C) the level of secreted IL4 and IFNγ from mesenteric lymph node cells of Mina KO and WT littermate control mice infected 21 days earlier with 150 embryonated TM eggs. Data are mean ± SD (A: WT n = 8, KO n = 10 each, B: WT and KO: n = 15 and 14 each respectively, C: WT and KO n = 7 each respectively) from 2 independent experiments). Statistical significance was computed by the two-tailed Student’s t-test.</p
Representative of ER stress genes deregulated in both the microarray from Siah1a/2 KO MEF cells and the published microarray performed on ATF4 KO MEF cells or PERK KO liver after TM treatment.
No full text<p>Representative of ER stress genes deregulated in both the microarray from Siah1a/2 KO MEF cells and the published microarray performed on ATF4 KO MEF cells or PERK KO liver after TM treatment.</p
Evaluation of second trimester maternal serum screening for Down’s Syndrome using the Spiegelhalter-Knill-Jones (S-KJ) approach
No full textSecond-trimester maternal serum levels of alpha-fetoprotein, free (-subunit of human chorionic gonadotropin and CA-125 in twin pregnancy after multifetal pregnancy reduction
No full textKua Hiri Mai Te Rā - A journey of self identification for the descendants of Ngāti Rāhiri ki Taranaki
No full textPrior to the arrival of Pakehā to the shores of Aotearoa, Māori retained and transmitted their histories, genealogies and protocols orally through such mediums as song and chant. The intracacies of the information held was such that every aspect of the Māori culture had its assigned expert whose job was to both retain and pass on their area of expertise. The settlement of Pakehā saw a change in how information was stored and disseminated, with a number of ethnographers, surveyors and historians recording accounts given to them from various Māori sources in written form. The main motivation for this at the time was to secure the information and cultural practises of a people who were deemed to be a dying race.
The implication that Māori were close to extinction was in vain, as many resisted against the gradual assimilation of Aotearoa (New Zealand) by the new foreign immigrants. The subsequent annexation of Aotearoa under British colonial rule eventually led to war against Māori, as Māori attempted to protect and retain both their lands and their identity. The result of the war led to a great loss of Māori lands, lives, and most importantly – identity.
Within Taranaki the Ngāti Rāhiri Hapū fought many generations for the right to stand unified as a people. Although they can be regarded as a small coastal hapū entity, they are at the forefront of maintaining and asserting their rangatiratanga status within today’s society. At the centre of the proclamation of rights and entitlement within their ancestral lands, questions have been raised that, for a number of generations, had baffled the likes of Elsdon Best and S. Percy Smith, as many sought to find answers regarding the true origins of Ngāti Rāhiri.
The impetus in undertaking this research is to ascertain who this prominent ancestor Rāhiri is from both a Ngā Puhi and Taranaki perspective, and investigate how these combined histories assist in the assertion of self by the Ngāti Rāhiri ki Taranaki people. By undertaking a critical review and analysis of literature, court minute records, hapū documents, proverbs, histories and knowledge from tribal elders pertaining to Rāhiri, this researcher will endeavour to provide and present relevant information and evidence, in a cohesive format, to show the close ancestral ties and affiliations between the two peoples. Furthermore, it is hoped that the research findings presented within this body of work may provide a significant contribution to the descendants of the Ngāti Rāhiri ki Taranaki Hapū, as they continue to assert their mana and rangatiratanga through the knowledge and understanding of self, when posed with the question - Ko wai a Ngāti Rāhiri
Properties of OCAM KO neurospheres.
No full text<p>(<b><i>A</i></b>): Immunofluorescence detection of OCAM in KO and WT embryonic spinal cord neurospheres. Scale bar = 10 μm. (<b><i>B</i></b>): Western blot analysis of OCAM in indicated protein extracts. Vector, TM and GPI indicates OCAM KO neurospheres which were infected with respectively empty, OCAM-TM cDNA and OCAM-GPI cDNA lentiviruses. β-actin was used as an internal control. (<b><i>C</i></b>): Differentiation of KO and WT cultures. The % of astrocytic, neuronal and oligodendrocytic cells detected by the indicated markers are indicated. No significant difference was observed (n = 10 fields). (<b><i>D</i></b>): Growth properties of KO neurospheres. <i>Left</i>: Cell numbers obtained 7 days after seeding of indicated cultures (n = 7 wells). <i>Right</i>: neurosphere forming cell unit (Nsfu) of indicated cultures (n = 4). (<b><i>E</i></b>): <i>Left</i>: percentage of Ki67<sup>+</sup> cells in KO and WT cultures (n = 6). <i>Middle</i>: QPCR quantification of Ki67/GAPDH mRNA (n = 4). <i>Right</i>: % of apoptotic cells detected by TUNEL assay. n.s. = not significant. (n = 4). (<b><i>F</i></b>): Cytometric analysis of OCAM expression in indicated cultures. Vector, TM and GPI indicates KO neurosphere cells which were transduced with respectively empty, OCAM-TM cDNA and OCAM-GPI cDNA lentiviruses. (<b><i>G</i></b>): Growth analysis of WT and KO neurospheres transduced by indicated lentiviruses. Cell numbers were measured 7 days after seeding (n = 7 wells). (<b><i>H</i></b>): Neurosphere forming assays of WT and KO neurospheres transduced by the indicated lentiviruses. Only OCAM-TM lentivirus decreased the Nsfu in both cultures (n = 4). Values represent relative Nsfu using control infected cells as the reference. (<b><i>I</i></b>): Effect of recombinant OCAM protein on cell growth. Cell numbers were measured after 7 days of growth of KO and WT cells in the presence of 7 μg/ml of OCAM-Fc protein or Fc fragment (n = 7).</p
TMEM165 KO cells showed reduced levels of glycosphingolipids.
No full text(A-B) Both a TMEM165 KO cell line (TM-KO-3) and a mutant cell line (TM-Mut-1, insertion of Cys-Tyr residues between Leu279 and Cys280) showed increased resistance to Stx1 (A) and Stx2 (B). (C) TM-KO-3 and TM-Mut-1 cells showed reduced sensitivities to ricin (10 ng/mL) compared with WT cells. (D-E) TM-KO-3 and TM-Mut-1 cells showed similar sensitivities to A-Dtx (D) (Student’s t test, p > 0.05), but reduced sensitivity to Ctx (E) compared with WT cells. (F) TM-KO-3 and TM-Mut-1 cells showed reduced binding of Stx1 and CtxB. Ectopic expression of TMEM165 (with an N-terminal triple HA tag) restored binding of Stx1 and CtxB in both cell lines. Scale bar, 5 μm. Representative images are from one of three independent experiments. (G) Mass spectrometry analysis revealed that TM-KO-3 and TM-Mut-1 cells have lower levels of Gb3, LacCer, GlcCer, Cer, and gangliosides (GM2) compared with WT cells. Quantification data are provided in S4 Data. All error bars indicate mean ± SD, N = 3.</p
Synthesis optimization and charge carrier transfer mechanism in LiLuSiO<sub>4</sub>:Ce, Tm storage phosphor
No full textLiLuSiO4:Ce and LiLuSiO4:Ce, Tm show very efficient charge carrier storage properties upon beta irradiation after samples have received treatment in vacuum. They outperform the commercial storage phosphor BaFBr(I):Eu2+ in many aspects. The influence of the synthesis conditions, Ce and Tm concentration, nonstoichiometry and codoping with Ca, Hf, Al and Ge are reported. Based on the results of the synthesis optimization, thermoluminescence (TL) emission and TL excitation spectra a mechanism of charge carrier transfer, storage, and recombination during irradiation and thermal or optical readout is proposed.Accepted Author ManuscriptRST/Fundamental Aspects of Materials and EnergyRST/Luminescence Material
Measurement of neutron cross sections and resonance parameters of Tm-169 below 101 eV
No full textThe neutron total cross-sections of thulium (Tm-169) were measured in the neutron energy region from 0.01 eV to 100 eV by using the time-of-flight method at the Pohang Neutron Facility, which consists of an electron liuac, a water-cooled tantalum target with a water moderator, and a 12 m time of flight path. Two thulium plates with different thicknesses were used for the neutron transmission measurement. The background level was determined by usiug a notch-filter of Co, In, and Cd sheets. The present measurement was compared with the previous ones, and a new set of resonance parameters of Tm-169 isotope was obtained from the transmission rate by using the SAMMY code, with a comparison with the recommended parameters by Mughabghab.11sciescopu
Total Cross-Section Measurements of (169)Tm below 100 eV
No full textThe neutron total cross-sections of thulium (Tm-169) were measured in the neutron energy region from 0.01 eV to 100 eV by using the time-of-flight method at the Pohang Neutron Facility, which consists of an electron linac, a water-cooled tantalum target with a water moderator, and a 12-m-long time-of flight path. Two thulium plates with different thicknesses were used for the neutron transmission measurement. The background level was determined by using a notch-filter of Co, In, and Cd sheets. The present measurement was compared with the previous ones, while a new set of resonance parameters of Tm-169 isotope was obtained from the transmission ratio by using the SAMMY code, with a comparison with the recommended parameters by Mughabghab.X111sciescopuskc
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