2,133 research outputs found
Validation and HH-responsiveness of <i>Gli</i> KO pancreatic fibroblast.
No full textlines A-B) qPCR analysis of Gli2 and Gli3 expression in WT (A, blue bars) Gli2/Gli3 KO (A, red bars), Gli1 KO (B, white bars) and Gli1/Gli2/Gli3 KO (B, red bars) pancreatic fibroblast lines. C-D) Western Blot analysis for GLI2 (C) and GLI3 (D) in Gli WT and Gli KO pancreatic fibroblast lines. Vinculin was used as a loading control. E-F) qPCR analysis for HH target genes Gli1 and Ptch1 in Gli2/Gli3 KO (E) and Gli1/Gli2/Gli3 KO (F) pancreatic fibroblasts following treatment with vehicle or SAG (600nM). P-values determined by ordinary one-way ANOVA with Tukey’s multiple comparison test. (TIF)</p
Sectoral allocation by gender of Latin American workers over the liberalization period of the 1990s
Get PDFThe recent restructuring of Latin American economies has renewed interest in the effects of trade liberalization, on labor markets, and on the gender division of labor. The author does not attempt to establish casuality between economic reforms, and the types of jobs that men and women hold. Instead, she provides a detailed description of the trends in male, and female formal, and informal sector participation during the economic reform period in Argentina, Brazil, and Costa Rica. The author first compares the gender composition of the formal, informal wage, and self-employment sectors in a year before reforms (1988 for Argentina, 1989 for Brazil, and Costa Rica), and a year after reforms implementation (1997 for Argentina, 1995 for Brazil and Costa Rica). Although women continued to be more likely than men to work in the informal wage sector, there is no trend of"masculinization"or"feminization"of the informal sector, or any other. Instead, in Argentina men have overtaken women as the most prevalent workers in the informal wage sector, while in Brazil, the opposite has occurred (as men move into self-employment). In Costa Rica there have been no statistical, observable changes. The author then considers the distribution across sectors within each gender group, to identify whether men, and women are more likely to select different sectors in the post-reform period relative to the pre-reform period. Among both men, and women in all three countries (except Brazilian men), workers have become more likely to hold informal wage jobs, and less likely to hold formal sector jobs. Trends in human capital accumulation explain these changes for both men, and women, while changes in gender roles, primarily in homecare and marriage, do not seem to have an effect.Health Monitoring&Evaluation,Labor Policies,Population&Development,Public Health Promotion,Environmental Economics&Policies,Health Monitoring&Evaluation,Environmental Economics&Policies,Population&Development,Banks&Banking Reform,Work&Working Conditions
Human herpesvirus-8 associated Kaposi’s sarcoma after lung transplantation: A case report
No full textBiochemical properties of a chitin-binding class III chitinase in pumpkin leaves
No full textWhen we compared the chitinase activity of various plant sources using colorimetric or active gel-staining assay methods, the specific activity of pumpkin leaves was the highest among the samples we analyzed. The highly active chitinase from pumpkin leaves (designated PL-ChtIII) was purified to homogeneity using affinity chitin gel and HPLC Mono-Q anion-exchange column chromatographies, In contrast to other members of the class III chitinase family, PL-ChtIII showed a strong binding affinity to the regenerated chitin gel column. The apparent molecular weight of PL-ChtIII was estimated to be 29 kDa on SDS-PAGE gel, while its optimum pH and temperature were shown to be pH 6.0 and 60 degrees C, respectively. Analyzing the reaction products of PL-ChtIII with swollen chitin as substrate, the dimer and tetramer of N-acetylglucosamine were produced as major products in the first hour of the enzymatic reaction along with a small amount of monomers and trimers, As the reaction time increased, dimeric N-acetylglucosamine became the predominant form of reaction product
<i>Gli3R</i> expression partially restored HH signaling dependent progenitors in <i>Ankmy2</i> ko hindbrain.
No full textTop, panels show bright-field images of wildtype (wt), Ankmy2 ko, Gli3R/+, Ankmy2 ko; Gli3R/+, Gli2 ko, Ankmy2; Gli2 double ko, and whole-mount embryos at E9.25 of the following genotypes: wild-type (n = 5), Ankmy2 ko (n = 5), Gli3R/+ (n = 4), Ankmy2 ko; Gli3R/+ (n = 4), Gli2 ko (n = 3), Ankmy2 ko; Gli2 ko (n = 3). Exencephaly (marked by asterisk) persists in Ankmy2 ko and double mutants. Scale: 500 μm. Bottom, panels show hindbrain neural tube horizontal sections immunostained using designated markers. All images are counterstained with DAPI. Scale: 100 μm. (TIF)</p
Effect of continuous intravenous epoprostenol therapy on advanced primary pulmonary hypertension in Taiwanese patients.
No full textShort-term intravenous iloprost for treatment of reperfusion lung oedema after pulmonary thromboendarterectomy.
No full textStructure-activity relationship studies on chalcone derivatives: potent inhibition of platelet aggregation.
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