1,532 research outputs found
Pharmacological activity of (-)-discretamine, a novel vascular alpha-adrenoceptor and 5-hydroxytryptamine receptor antagonist, isolated from Fissistigma glaucescens.
No full textPharmacological evaluation of ocoteine, isolated from Cassytha filiformis, as an alpha 1-adrenoceptor antagonist in rat thoracic aorta.
No full textGeneration and analysis of pFN KO and dKO mice.
No full text(A) pFN KO were generated by breeding Fn(fl/fl) mice with Alb-Cre/+ transgenic mice in several breeding rounds. (B) Immunoblot for FN using mouse plasma isolated from control and pFN KO mice confirmed the absence of pFN in pFN KO mice (arrow). All mice were 30 days old at the time of blood collection (n = 10). (C) Generation of dKO mice by breeding Fn(fl/fl); Sma-Cre-ERT2/+ with Fn(fl/fl); Alb-Cre/+ mice. After injection with tamoxifen from P1–P3, the dKO mice lack total pFN and cFN secreted by SMCs. (D) Hemorrhages in the thoracic cavity of dead dKO mice (P3 and P5) suggest a vascular cause of lethality (left and middle panel). The age-matched control pFN KO mice (found dead) do not show this phenotype (right panel). Animals of each group are shown in the top panel at 3× and in the bottom panel at 4.5× magnification. Scale bars represent 1 mm. (E and F) Representative immunostained images of aortic walls from P1–P3 tamoxifen-injected control mice and dKO mice at P8 with antibodies against total FN (pFN and cFN) (E) and against EDA FN (specific for cFN) n = 4 (F). Blue staining represents nuclei counterstaining with DAPI. Quantification of the red FN signals is shown at the bottom of each figure and the underlying data are provided in S1 Data. Note the significantly reduced levels of both total FN and EDA FN in the media (arrowheads). Lumen is indicated with an asterisk, and scale bar represents 50 μm in (E) and (F). cFN, cellular fibronectin; dKO, double knockout; EDA, extra domain-A; FN, fibronectin; KO, knockout; pFN, plasma fibronectin; SMC, smooth muscle cell.</p
A combination therapy of androgen and estrogen receptor modulators improves bone healing in a rat model of postmenopausal osteoporosis
No full textVasoconstricting effect in rat aorta caused by thaliporphine isolated from the plant Neolitsea konishii K.
No full textActive Site Structure and Sterospecificity of Escherichia coli Pyridoxine 5'-phosphate Oxidase
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Vasorelaxing effect in rat thoracic aorta caused by fraxinellone and dictamine isolated from the Chinese herb Dictamnus dasycarpus Turcz: comparison with cromakalim and Ca2+ channel blockers.
No full textPharmacological characteristics of liriodenine, isolated from Fissistigma glaucescens, a novel muscarinic receptor antagonist in guinea-pigs.
No full textAbnormal expressions of extracellular matrix proteins in the kidneys of Gpr48 KO mice.
No full text<p>Some important fibrotic markers are highly expressed in the Gpr48 KO mice compared with the WT mice. (a) Total RNA was extracted and analyzed for the expression of collagenI, III, IV, MMP1,2,9, TIMP1,2 CTGF, FN and alpha-SMA in the kidneys by RT-PCR. (b) Western blot analysis depicted protein level of collagenI, IV, MMP2 and alpha-SMA in the kidneys of mice. (c) The expression of collagen I, IV, MMP2 and FN proteins was studied by immunohistochemistry on Gpr48 KO and WT adult mice (Magnification, 400×).</p
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