95 research outputs found
Molecular Genetic Methods for Detection and Identification of Viable but Nonculturable Microorganisms
No full textAutomated Classification and Cluster Visualization of Genotypes Derived from High Resolution Melt Curves.
No full textHigh Resolution Melting (HRM) following PCR has been used to identify DNA genotypes. Fluorescent dyes bounded to double strand DNA lose their fluorescence with increasing temperature, yielding different signatures for different genotypes. Recent software tools have been made available to aid in the distinction of different genotypes, but they are not fully automated, used only for research purposes, or require some level of interaction or confirmation from an analyst.We describe a fully automated machine learning software algorithm that classifies unknown genotypes. Dynamic melt curves are transformed to multidimensional clusters of points whereby a training set is used to establish the distribution of genotype clusters. Subsequently, probabilistic and statistical methods were used to classify the genotypes of unknown DNA samples on 4 different assays (40 VKORC1, CYP2C9*2, CYP2C9*3 samples in triplicate, and 49 MTHFR c.665C>T samples in triplicate) run on the Roche LC480. Melt curves of each of the triplicates were genotyped separately.Automated genotyping called 100% of VKORC1, CYP2C9*3 and MTHFR c.665C>T samples correctly. 97.5% of CYP2C9*2 melt curves were genotyped correctly with the remaining 2.5% given a no call due to the inability to decipher 3 melt curves in close proximity as either homozygous mutant or wild-type with greater than 99.5% posterior probability.We demonstrate the ability to fully automate DNA genotyping from HRM curves systematically and accurately without requiring any user interpretation or interaction with the data. Visualization of genotype clusters and quantification of the expected misclassification rate is also available to provide feedback to assay scientists and engineers as changes are made to the assay or instrument
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Union Seminary Quarterly Review, volume 19, issue 2 (1964)
No full text"The Reformation .of the Church" by John C. Bennett, 99
"Reflections:on the Negro Revolt" by Henry B. Clark II, 107
A Christmas Triptych" by Frederick Buechner, 123
THE INAUGURAL PRESENTATIONS
"Conscience" by James A. Knight, 131
"What Psychiatry Means to Theological Education" by Daniel Day Williams, 141
"The Roots of the Reconciling Message" by Ralph W. Sockman, 145
"Book Reviews," 153
Christopher M. Brookfield, John L. Casteel, W. Richard Comstock, Brian A. Gerrish, John A. Gettier, Robert G. Hamerton Kelly, Robert T. Handy, Clair Johnson, Marshall D. Johnson, J..Kenneth Kuntz, James Muilenburg, John C. Raines, Cyril C. Richardson, Roger L. Shinn, Sidney Skirvin, James D. Smart, Ivor B. Thomas, Mary A. Tully, George W. Webber, Richard James Woo
The Current State of Robot-Assisted Minimally Invasive Esophagectomy (RAMIE): Outcomes from the Upper GI International Robotic Association (UGIRA) Esophageal Registry
No full text\ua9 The Author(s) 2024.Background: Robot-assisted minimally invasive esophagectomy (RAMIE) is increasingly adopted in centers worldwide, with ongoing refinements to enhance results. This study aims to assess the current state of RAMIE worldwide and to identify potential areas for improvement. Methods: This descriptive study analyzed prospective data from esophageal cancer patients who underwent transthoracic RAMIE in Upper GI International Robotic Association (UGIRA) centers. Main endpoints included textbook outcome rate, surgical techniques, and perioperative outcomes. Analyses were performed separately for intrathoracic (Ivor–Lewis) and cervical anastomosis (McKeown), divided into three time cohorts (2016–2018, 2019–2020, 2021–2023). A sensitivity analysis was conducted with cases after the learning curve (> 70 cases). Results: Across 28 UGIRA centers, 2012 Ivor–Lewis and 1180 McKeown procedures were performed. Over the time cohorts, textbook outcome rates were 39%, 48%, and 49% for Ivor–Lewis, and 49%, 63%, and 61% for McKeown procedures, respectively. Fully robotic procedures accounted for 66%, 51%, and 60% of Ivor–Lewis procedures, and 53%, 81%, and 66% of McKeown procedures. Lymph node yield showed 27, 30, and 30 nodes in Ivor–Lewis procedures, and 26, 26, and 34 nodes in McKeown procedures. Furthermore, high mediastinal lymphadenectomy was performed in 65%, 43%, and 37%, and 70%, 48%, and 64% of Ivor–Lewis and McKeown procedures, respectively. Anastomotic leakage rates were 22%, 22%, and 16% in Ivor–Lewis cases, and 14%, 12%, and 11% in McKeown cases. Hospital stay was 13, 14, and 13 days for Ivor–Lewis procedures, and 12, 9, and 11 days for McKeown procedures. In Ivor–Lewis and McKeown, respectively, the sensitivity analysis revealed textbook outcome rates of 43%, 54%, and 51%, and 47%, 64%, and 64%; anastomotic leakage rates of 28%, 18%, and 15%, and 13%, 11%, and 10%; and hospital stay of 11, 12, and 12 days, and 10, 9, and 9 days. Conclusions: This study demonstrates favorable outcomes over time in achieving textbook outcome after RAMIE. Areas for improvement include a reduction of anastomotic leakage and shortening of hospital stay
Automated genotyping procedure.
No full text<p>A. Fluorescence (<i>F</i>) versus temperature (<i>T</i>). B.–<i>dF/dT</i> versus <i>T</i>. C. Temperature shifted–<i>dF/dT</i>. D. Normalized–<i>dF/dT</i> curves with training set genotype averages (black lines). E. A 3D point represents each curve correlated against each average curve. F. Points transformed to spherical coordinates. G. Genotype likelihood table H. 2D projection of correlation parameters for visualization.</p
Comparative and competitive advantages of drug trafficking in the Southeastern Caribbean Islands: a comparative study of Trinidad and Tobago. St. Vincent and the Grenadines., 2012
No full textThe purpose of this study was to examine drug trafficking and the major challenges that it poses to Caribbean development, particularly the cases of St. Vincent and the Grenadines and Trinidad and Tobago. The analysis offered, expresses the specific challenges to SVG and T&T due to the proliferation of DTPOs. The studys goals were: 1) To ascertain to what extent is drug trafficking a major problem for the region, 2) To identify what are the comparative and competitive determinants of drug trafficking in the Southeastern Caribbean islands, 3) To establish to what extent does supply and demand become important to the Drug Trade in the Caribbean, and Finally, 4) the identify the implications of this research for the Greater Caribbean region. A comparative evaluation of Caribbean political economy was used in conducting this research. The primary data collection was administered through Elite interviews with policy makers and officials in the Case study countries of Trinidad and Tobago and St. Vincent and the Grenadines. 26 interviews were conducted over a 6 month period be 2011. This study discusses the political economy of the illegal narcotics trade among the small island states of the Southeastern Caribbean. It argues that the growth of drug trafficking operations is the product of the comparative and competitive advantages that these islands economies benefit from globally, relative to other states
Comprehensive DNA signature discovery and validation.
No full textDNA signatures are nucleotide sequences that can be used to detect the presence of an organism and to distinguish that organism from all other species. Here we describe Insignia, a new, comprehensive system for the rapid identification of signatures in the genomes of bacteria and viruses. With the availability of hundreds of complete bacterial and viral genome sequences, it is now possible to use computational methods to identify signature sequences in all of these species, and to use these signatures as the basis for diagnostic assays to detect and genotype microbes in both environmental and clinical samples. The success of such assays critically depends on the methods used to identify signatures that properly differentiate between the target genomes and the sample background. We have used Insignia to compute accurate signatures for most bacterial genomes and made them available through our Web site. A sample of these signatures has been successfully tested on a set of 46 Vibrio cholerae strains, and the results indicate that the signatures are highly sensitive for detection as well as specific for discrimination between these strains and their near relatives. Our approach, whereby the entire genomic complement of organisms are compared to identify probe targets, is a promising method for diagnostic assay development, and it provides assay designers with the flexibility to choose probes from the most relevant genes or genomic regions. The Insignia system is freely accessible via a Web interface and has been released as open source software at: http://insignia.cbcb.umd.edu
Separation of <i>MTHFR C</i>.<i>665C>T</i> genotypes using data from different temperature ranges.
No full text<p>Left: 54 to 87C (probe and amplicon), middle: 54 to 74C (probe only) and right: 74 to 87C (amplicon only). Top: normalized derivative curves, middle: separation of genotype clusters in 2D. Bottom row shows the expected probability cross table via Monte Carlo simulation of 3D spherical coordinates.</p
Nasilja velikaša i plemstva u srednjovjekovnoj Slavoniji tijekom 14. stoljeća
No full textNasilje je komponenta ljudskog života koja prati sve oblike ljudskih zajednica kroz
prošlost. Silništva koja su se provodila tijekom srednjeg vijeka bila su ob
razac svakodnevice
tadašnjih ljudi. Srednjovjekovni život prožet je afektivnim osobinama koje se u ljudima
javljaju na mnogo izraženiji način nego što je uobičajeno u današnje vrijeme. To vrijedi za
negativna i pozitivna svojstva ponašanja. Negativne znača
jke poput gramzljivosti, sebičnosti,
borbenosti, raspaljivosti, oholosti i mržnje mogu se dovesti u izravnu vezu s međuljudskim
sukobljavanjem.
1
Koja je svrha istraživanja sukoba? Cilj istraživanja sukoba je spoznaja o
načinu funkcioniranja određenog društ
va. Kojim se pravilima to društvo podređuje, do koje su
mjere prihvaćena te na koji je način normirano razrješavanje sukoba?
2
Odgovore na ova
pitanja pokušat ću dati u ovom radu. Iako je nasilje činilo takvu važnu sastavnicu cjelokupnog
funkcioniranja ljud
skih zajednica, u hrvatskoj je historiografiji ono relativno slabo istraženo.
Općenito, može se primijetiti kako je područje srednjovjekovne Hrvatske i Dalmacije bolje
obrađeno. S druge strane, srednjovjekovna je Slavonija slabije zastupljena te se na ovak
ve
probleme povjesničari najviše obaziru u sintezama. Zbog toga sam dobio poticaj za
obrađivanje ove specifične teme. Kao što govori naslov, problematika će se ograničiti na
područje srednjovjekovne Slavonije tijekom 14. stoljeća. Srednjovjekovna Slavonija
izabrana
je upravo zbog slabije obrađenosti, dok je 14. stoljeće predmetom istraživanja zbog posebnih
procesa koji se zbivaju tijekom tog razdoblja. To je stoljeće razdoblje razvijenoga kasnog
srednjeg vijeka, koje donosi promjene u odnosu na dotadašnji t
ijek razvoja Ugarsko
-
hrvatskoga kraljevstva. Brojni procesi, događanja i razvitci pridonose zanimljivosti tog
stoljeća. Smjena dinastija, dolazak anžuvinske na mjesto arpadovićevske, koja nestaje s
povijesne pozornice nakon dvostoljetnog vladanja hrvatskim
zemljama. Borbe, sukobi,
neprijateljstva, savezništva i sklapanje novih društvenih obrazaca koji prate takve važne
političke i društvene događaje obilježja su cjelokupnog srednjovjekovlja. Takva previranja
osobito će doći do izražaja na prijelazu iz 13. u
14. stoljeće.In this thesis paper, the author examines the violence of lords and other members of
nobility during the fourteenth century in Slavonia.
The author's primary goals were to determine the forms of violence that ruled during
that century and how they coincide with political and social changes that occured during the
fourteenth century. To explain the background of violence on that specific territory and the
kind of society that is in focus of this paper, the author explains the extent of medieval
Slavonia and its social structure.
Many changes that occured during the fourteenth century had direct impact on
administrative, judicial, and daily life of medieval people. Following this the author gives
development survey of customs that were still in practice in medieval Slavonia. Also it is
given an overview of evolution in written laws and regulations that were increasing during
fourteenth century. That kind of development coincides with the arrival of new dynasty,
Anjou, in Hungarian – Croatian Kingdom.
Political changes in this century included conflicts, political consolidation, wars,
decline and rise of lords. In the background of this events changes were occuring that affected
everyday life. Changes that included violence and remained important aspect of medieval life.
Violence continued to exist in all its forms during fourteenth century. Author separates
violence in different forms and analyzes them segment per segment. The paper establishes
that different forms of violence were always common regardless of political situtations. The
main difference in the fourteenth century and the early medieval ages lies in the development
of legal state and regal authority, that had the impact on finding and punishing the offenders.
The success of developing effective government in the fourteenth century will be short lived
because in the end of this century, first signs of declining regal authority were showing and it
would never again regain that kind of power until the sixteenth century
A general history of Hampshire or the county of Southampton : including the isle of Wight /
No full textPlates by C. Cousen, E. Brandard, J. C. Armytage, W. Wallis, S. Bradshaw, J. Saddler, E. Challis, J. H. Le Keux, J. Cleghorn, A. Willmore, E. J. Roberts, C. Hall, F. W. Fairholt, W. J. Edwards, T. W. Knight, W. Roffe, E. Albutt, W. Hughes, J. J. Crew, H. Bourne, Geo. Stodart, J. Cousen, W. Mossman, F. Smith, J. W. Lowry, J. Basire, J. Godfrey, E. Finden, and Charles Cousen; after W. H. Bartlett, J. C. Armytage, J. Cleghorn, I. Closterman, F. Cotes, Sir Joshua Reynolds, Thos. Philips, J. O. Westwood, Nash, J. Basire, E. H. Corbould, E. W. Cooke, W. J. Cook, Charles Cousen, A. Simms, and R. Brandard.British Art Center has 2 copies. V. 1 is a large paper copy and has the bookplates of T. Homer-Saunders and Herbert Walford. Copy 2 is imperfect: Supplement wanting. Copy 2 is extra-illustrated with prints, photographs and newspaper clippings and has the bookplate of Ivor A. B. Ferguson.Vol. 1: [2], ix, [1], 416 p.; v. 2: [8], 374 p.; v. 3: [8], 354, 82, 29 p."Virtue and Co., Printers, City Road, London."Preface signed by Wilks, who continued Woodward's unfinished work. Lockhart's contribution is called a Supplement.Added t.-p., engraved.v. 1. Winchester and Romsey, and the adjacent country. p. 1-317 by B. B. Woodward, p. 318-418 by T. C. Wilks.--v. 2. Southampton and Alresford, and the adjacent country. By T. C. Wilks.--v. 3. The New forest. Christchurch, Andover and the country east of Winchester, etc., by T. C. Wilks. The isle of Wight by C. Lockhart.Mode of access: Internet
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