43,636 research outputs found
The archaeoacoustics of San Vitale, Ravenna
No full textThis research tests and assesses whether sixth-century social and cultural dynamics can be archaeologically identified by including the study of acoustics in the context of extant Late Antique Christian architecture, namely the centrally planned domed octagonal church of San Vitale at Ravenna. Implementing a holistic archaeological research strategy that includes human sensory perception of acoustical phenomena is the best approach to unravelling the complexities of social and cultural mechanisms operating in the sixth-century Mediterranean basin. The methods and issues of Archaeoacoustics are critiqued and developed in order to comment on the intentionality of acoustic attributes in sixth-century ecclesiastical architecture.The space syntax of San Vitale has been considered for isovists at key locations during the liturgical procession and sequence of the Mass celebration. These are compared with mapped areas of perceiving the acoustic characteristics of Clarity and Reverberation Time. Combining the visual and acoustic analysis of San Vitale, with a better understanding of its date and construction phases, the physical geometry and temporal logic of the church are discussed in relation to the reflexive exchange of influence between Ravenna, Milan and Constantinople. It is posited that liturgical and musical time and tempo is materially expressed in the evident and conceptual substance of San Vitale, a suggestion that offers a springboard for future study and debate
Les mystères de la procession de Lille, éd. critique par Alan E. Knight, t. I, Le Pentateuque, t. II, De Josué à David
No full textBonicel Matthieu. Les mystères de la procession de Lille, éd. critique par Alan E. Knight, t. I, Le Pentateuque, t. II, De Josué à David. In: Bibliothèque de l'école des chartes. 2004, tome 162, livraison 1. pp. 237-239
Les Mystères de la procession de Lille, éd. crit. par Alan E. Knight ; t. I, Le Pentateuque, 2001 ; t. II, De Josué à David, 2003
No full textBordier Jean-Pierre. Les Mystères de la procession de Lille, éd. crit. par Alan E. Knight ; t. I, Le Pentateuque, 2001 ; t. II, De Josué à David, 2003. In: Romania, tome 123 n°489-490, 2005. pp. 239-244
Les mystères de la procession de Lille, éd. critique par Alan E. Knight, t. I, Le Pentateuque, t. II, De Josué à David
No full textBonicel Matthieu. Les mystères de la procession de Lille, éd. critique par Alan E. Knight, t. I, Le Pentateuque, t. II, De Josué à David. In: Bibliothèque de l'école des chartes. 2004, tome 162, livraison 1. pp. 237-239
$150.00 REWARD On Monday morning, April 21, 1892 Grover Orvis shot and killed Jacob Knight
No full text$150.00 REWARD
On Monday morning, April 21, 1892 Grover Orvis shot and killed Jacob Knight, an Indian preacher, near McDermott. The above reward will be paid for the capture of Grover Orvis and his father, C. M. Orvis.
Grover Orvis is about five feet eight inches, weighs about140lbs, smooth face. light complexion,
blue eyes, age about 21 years. C. M. Orvis is about five feet 10 inches, about 55 years old, light
complexion, heavy gray mustache, blue eyes, very slim face, weight
about 180 pounds. For further information or particulars,
Address:
F. M. RILEY,
McDermott, I. T.
April 25, 1902
Primary cilia elongation in response to interleukin-1 mediates the inflammatory response
No full textPrimary cilia are singular, cytoskeletal organelles present in the majority of mammalian cell types where they function as coordinating centres for mechanotransduction, Wnt and hedgehog signalling. The length of the primary cilium is proposed to modulate cilia function, governed in part by the activity of intraflagellar transport (IFT). In articular cartilage, primary cilia length is increased and hedgehog signaling activated in osteoarthritis (OA). Here, we examine primary cilia length with exposure to the quintessential inflammatory cytokine interleukin-1 (IL-1), which is up-regulated in OA. We then test the hypothesis that the cilium is involved in mediating the downstream inflammatory response. Primary chondrocytes treated with IL-1 exhibited a 50 % increase in cilia length after 3 h exposure. IL-1-induced cilia elongation was also observed in human fibroblasts. In chondrocytes, this elongation occurred via a protein kinase A (PKA)-dependent mechanism. G-protein coupled adenylate cyclase also regulated the length of chondrocyte primary cilia but not downstream of IL-1. Chondrocytes treated with IL-1 exhibit a characteristic increase in the release of the inflammatory chemokines, nitric oxide and prostaglandin E2. However, in cells with a mutation in IFT88 whereby the cilia structure is lost, this response to IL-1 was significantly attenuated and, in the case of nitric oxide, completely abolished. Inhibition of IL-1-induced cilia elongation by PKA inhibition also attenuated the chemokine response. These results suggest that cilia assembly regulates the response to inflammatory cytokines. Therefore, the cilia proteome may provide a novel therapeutic target for the treatment of inflammatory pathologies, including OA
B cells, T cells and sepsis: understanding the adaptive immune response
No full textSepsis is defined as life-threatening organ dysfunction resulting from a dysregulated host response to infection. Globally, sepsis is responsible for 11 million deaths each year and is the most common reason for admission to adult intensive care units (ICUs) in the UK. Despite its significant impact, there are currently no sepsis specific treatments targeting the host response. Sepsis is associated with high mortality, estimated at 30% for the initial episode, and around 15% of survivors die within the following year. Healthcare-associated or secondary infections and recurrent sepsis are common causes of long-term mortality, suggesting that persistent alterations in adaptive immunity contribute to the detrimental effects of sepsis. A greater understanding of underlying disease mechanisms is paramount for reducing the associated socio-economic burden.
To elucidate the adaptive immune response in sepsis, I have conducted the first comprehensive longitudinal study of the B and T cell receptor (B/TCR) repertoire using high-throughput multiplex PCR and next generation sequencing (NGS). Seventy-two patients with sepsis and twelve matched healthy controls were sampled at three time points, days one, three and five, post-ICU admission to understand the dynamic immune response. Patients were recruited as part of the UK Genomic Advances in Sepsis (GAinS) study.
As part of this thesis, I developed an optimised data dimensional reduction pipeline called repertoire (r) WGCNA which utilises weighted gene correlation network analysis. I demonstrate how rWGCNA can be used to identify a small number of modules which capture the combined effect of several correlated immune repertoire features (e.g. diversity and V gene usage). By applying conventional statistical analysis, I used these modules to characterise the alterations to the adaptive immune repertoire during sepsis, effectively distinguishing between health and disease. Additionally, I identified repertoire features with significant associations to clinical variables, Sepsis Response Signatures (SRS), and mortality, focusing on novel biology and generating hypotheses for future research.
Finally, by integrating matched bulk RNA sequencing data from whole blood, I identified genes and pathways associated with various aspects of the adaptive immune response, including clonal expansion and diversification. These findings provide a foundation for future research aimed at understanding the pathophysiology of sepsis
Plagiognathus crocinus Knight 1927
No full text<i>Plagiognathus crocinus</i> Knight, 1927: 12 (n. sp.). <p>HOLOTYPE: Female: ‘‘ Bluemont, Va., July 1, 1914, WL McAtee Collector’ ’. Deposited in the National Museum of Natural History, Washington, D.C.</p> <p> The species was apparently described on the basis of a single female. The holotype is in the National Museum of Natural History, Washington, D.C. The general coloration, including all appendages, is yellow­orange; the clavus is narrowly darkened along the scutellum and claval commissure. The tibiae are yellowish and have no dark spots at the bases of the tibial spines although the spines themselves are dark. The structure of the head is <i>Plagiognathus</i> ­like, but the list of attributes given makes association with any described species difficult on the basis of the single female specimen.</p>Published as part of <i>SCHUH, RANDALL T., 2001, Revision Of New World Plagiognathus Fieber, With Comments On The Palearctic Fauna And The Description Of A New Genus (Heteroptera: Miridae: Phylinae), pp. 1-267 in Bulletin of the American Museum of Natural History 2001 (266)</i> on page 254, DOI: 10.1206/0003-0090(2001)266<0001:RONWPF>2.0.CO;2, <a href="http://zenodo.org/record/5381844">http://zenodo.org/record/5381844</a>
Exploiting knowledge of immune selection in HIV-1 to detect HIV-specific CD8 T-cell responses
No full textSince HLA-restricted cytotoxic T-cell responses select specific polymorphisms in HIV-1 sequences and HLA diversity is relatively static in human populations, we investigated the use of peptide epitopes based on sites of HLA-associated adaptation in HIV-1 sequences to stimulate and detect T-cell responses ex vivo. These "HLA-optimised" peptides captured more HIV-1 Nef-specific responses compared with overlapping peptides of a single consensus sequence, in interferon-γ enzyme linked immunospot assays. Sites of immune selection can reveal more immunogenic epitopes in HLA-diverse populations and offer insights into the nature of HLA-epitope targeting, which could be applied in vaccine design
Heterogeneous and tissue-specific regulation of effector T cell responses by IFN-gamma during Plasmodium berghei ANKA infection.
No full textIFN-γ and T cells are both required for the development of experimental cerebral malaria during Plasmodium berghei ANKA infection. Surprisingly, however, the role of IFN-γ in shaping the effector CD4(+) and CD8(+) T cell response during this infection has not been examined in detail. To address this, we have compared the effector T cell responses in wild-type and IFN-γ(-/-) mice during P. berghei ANKA infection. The expansion of splenic CD4(+) and CD8(+) T cells during P. berghei ANKA infection was unaffected by the absence of IFN-γ, but the contraction phase of the T cell response was significantly attenuated. Splenic T cell activation and effector function were essentially normal in IFN-γ(-/-) mice; however, the migration to, and accumulation of, effector CD4(+) and CD8(+) T cells in the lung, liver, and brain was altered in IFN-γ(-/-) mice. Interestingly, activation and accumulation of T cells in various nonlymphoid organs was differently affected by lack of IFN-γ, suggesting that IFN-γ influences T cell effector function to varying levels in different anatomical locations. Importantly, control of splenic T cell numbers during P. berghei ANKA infection depended on active IFN-γ-dependent environmental signals--leading to T cell apoptosis--rather than upon intrinsic alterations in T cell programming. To our knowledge, this is the first study to fully investigate the role of IFN-γ in modulating T cell function during P. berghei ANKA infection and reveals that IFN-γ is required for efficient contraction of the pool of activated T cells
- …
