12,989 research outputs found
A hybrid algorithm for global self-localization of indoor mobile robots with 2-D isotropic ultrasonic receivers
No full textDecentralised control for multi-channel active vibration isolation (in special Issue on dynamics and control of smart structures)
No full textThis paper describes a theoretical and experimental investigation into an active four-mount vibration isolation system, in which electromagnetic actuators are installed in parallel with each of the four passive mounts placed between a three-dimensional piece of equipment and a vibrating base structure. Decentralized velocity feedback control is applied, where each actuator is operated independently by feeding back the corresponding equipment vibration velocity at the same location. Although one end of the actuator acts at the sensor position on the equipment, the system is not collocated because of the reactive force at the other end acting on the flexible base structure, whose dynamics may be strongly coupled with the mounted equipment. The investigation of this actuator installation and its practical implementation are the motivation of this research. Isolation of low-frequency vibration is considered where the equipment can be modeled as a rigid body and the mounts as lumped-parameter springs and dampers. A general theoretical formulation for analysing multiple-mount vibration isolation systems using the impedance method is presented and is used to investigate the control mechanisms involved. Experimental results show that up to 14 dB reduction in the kinetic energy of the equipment can be achieved in practice. If very high gains are used in the experiments, however, instability occurs at low frequencies due to phase shifts in the transducer conditioning electronics
Compact and thermosensitive nature-inspired micropump
No full textLiquid transportation without employing a bulky power source, often observed in nature, has been an essential prerequisite for smart applications of microfluidic devices. In this report, a leaf-inspired micropump (LIM) which is composed of thermo-responsive stomata-inspired membrane (SIM) and mesophyll-inspired agarose cryogel (MAC) is proposed. The LIM provides a durable flow rate of 30 mu l/h . cm(2) for more than 30 h at room temperature without external mechanical power source. By adapting a thermo-responsive polymer, the LIM can smartly adjust the delivery rate of a therapeutic liquid in response to temperature changes. In addition, as the LIM is compact, portable, and easily integrated into any liquid, it might be utilized as an essential component in advanced hand-held drug delivery devices.116Ysciescopu
Screening of apoptotic genes by morphological analysis of high throughput fluorescence microscopy images
No full textA quantification method for fault coverage of NPP digital systems combining mathematical analysis and computer simulation
No full text[No abstract available
Aquatic flower-inspired cell culture platform with simplified medium exchange process for facilitating cell-surface interaction studies
No full textWalter E. Oberer Retirement Luncheon
No full textDean Oberer was emeritus of law and former dean of the S.J. Quinney College of Law. He received the Burlington Resources Foundation Faculty Achievement Award in 1991 and was particularly noted for his scholarship in labor law and was co-author of a book, "Cases and Materials on Labor Law: Collective Bargaining in a Free Society.
Cytogenetic analysis of chimeric antibody-producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure
No full textPreviously, the highest producing (HP) recombinant CHO subclones isolated at various methotrexate (MTX) levels showed different antibody production stability during long-term culture, although they were clonally derived from CS13 transformant. In this study, genetic basis for their difference in antibody production stability was investigated using southern blot hybridization and fluorescence in situ hybridization (FISH) techniques. Southern analysis of HP subclones revealed that light-chain (LC) and heavy- chain (HC) cDNAs were located closely within 23 kb on an amplification unit, and the configuration of LC and HC cDNAs within this amplification unit was not disrupted during long-term culture in the absence of MTX. However, when LC and HC genes were localized on the metaphase chromosomes of HP subclones using FISH, the amplified sequences were present as an extended array on diverse marker chromosomes. HP subclones selected at higher MTX level had more kinds of marker chromosomes. CS13*-002 isolated at 0.02 μM MTX had only one marker chromosome (m002), whereas CS13*-1.0 isolated at 1 μM MTX had five different ones (m10A, m10B, m10C, m10D, and m10E). Each marker chromosome showed different fate during long-term culture of HP subclones in the absence of MTX, resulting in different degrees of stability among the HP subclones. The m10A and m10B remained unchanged, whereas the others disappeared or evolved to variants with shortened amplified arrays. The cells containing stable marker chromosomes constituted dominant subpopulations in CS13*-1.0, and thereby CS13*-1.0 became most stable in regard to antibody production during long-term culture. Furthermore, our dual-color FISH showed that the telomeric ends of amplified arrays on the stable marker chromosomes were always surrounded by (TTAGGG)(n) sequences, indicating that (TTAGGG)(n) sequences are closely related to the stability and evolution of amplified sequences. Taken together, our data show that the assessment of genotypic stability of amplified CHO cells is a prerequisite for understanding their production stability during long-term culture in the absence of selection pressure
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