11,832 research outputs found
Expression of an endoxylanase gene in a tandem unit and effect of different replication mode on its structural stability in Bacillus subtils DB104
No full textSupplemental material for DWI-pc-ASPECT score in basilar artery occlusion: is 6 points or less always indicative of a bad outcome?
No full textSupplemental Material for DWI-pc-ASPECT score in basilar artery occlusion: is 6 points or less always indicative of a bad outcome? by Joong-Goo Kim, Dongwhane Lee, Jay Chol Choi, Yunsun Song, Deok Hee Lee and Dae Chul Suh in Interventional Neuroradiology</p
Thermomechanical properties of ABS/PA AND ABS/PC blends
No full textThe significant increase of Waste Electric and Electronic Equipment (WEEE) has led to an important research in upgrading recycled engineering plastics by means of a blending technique. Classical twin-screw extrusion is compared to a new blending technique, where two polymers are combined together and then flow in several static mixers. This technique allows to obtain different morphologies of compatibilized ABS/PA or ABS/PC blends and the aim of this work is to evaluate the corresponding thermomechanical properties. Experimental results demonstrate in particular that fibrilar morphology is obtained with the new blending technique (only with ABS/PA blends), which could lead to a mechanical enhancement if the adhesion between ABS and PA is optimized
Molecular cloning and characterization of an endoxylanase gene of Bacillus sp. in Escherichia coli
No full textA gene encoding an endoxylanase of Bacillus sp. was cloned acid expressed in Escherichia coli. The entire nucleotide sequence of a 1,620 bp SmaI fragment containing the endoxylanase gene was determined. The endoxylanase gene was 639 bp long and encoded 213 amino acids which showed up to 96% amino acid homology with other endoxylanases. The encloxylanase produced by E. coli harboring pKJX4 was purified by ion-exchange chromatography (DE-52 and CM-Si) and its N-terminal sequence was determined to be Ala-Gly-Thr-Asp-Tyr-Trp-Gln-Asn-Trp-Thr-Asp-Gly-Gly-Gly-Thr. The endoxylanase expressed in E. coli was identical to that of the riginal Bacillus sp, whose molecular weight was approximately 20,400. Most of the produced endoxylanase was localized in the periplasmic space of E. coli. When the endoxylanase was reacted with 2% oat spelts xylan (w/v) at 40 degrees C for 10 h, the major product was xylobiose which is known to be a selective growth stimulant to one of the healthy intestinal microflora, Bifidobacteria. (C) 1998 Elsevier Science Inc
A PC parallel port button box provides millisecond response time accuracy under Linux
No full textFor psychologists, it is sometimes necessary to measure people's reaction times to the nearest millisecond. This article describes how to use the PC parallel port to receive signals from a button box to achieve millisecond response time accuracy. The workings of the parallel port, the corresponding port addresses, and a simple Linux program for controlling the port are described. A test of the speed and reliability of button box signal detection is reported. If the reader is moderately familiar with Linux, this article should provide sufficient instruction for him or her to build and test his or her own parallel port button box. This article also describes how the parallel port could be used to control an external apparatus
Periodic orbits and non-integrability of Armbruster-Guckenheimer-Kim potential
No full textAgraïments: The second author is partially supported by HBP-2009-0025-PC and CAPES/MECD-DGU 015/2010 Brazil and Spain, process number BEX 4251/10-5.In this paper we study the periodic orbits of the Hamiltonian system with the Armburster-Guckenheimer-Kim potential and its non-integrability in the sense of Liouville-Arnold
Heavy pentaquark states Pc(4380) and Pc(4450) in the J/ψ production induced by pion beams off the nucleon
No full textAbstractIn this study, we investigate the J/ψ production induced by pion beams off the nucleon, particularly the heavy pentaquarks Pc(4380) and Pc(4450) in intermediate states, based on a hybridized Regge model. The process involving ρ and π meson exchange in the t channel is considered as background, and the heavy pentaquark exchange is included in the s channel. The coupling constants such as the ρNN and πNN vertices are taken from the NN potentials, whereas those for the J/ψρπ and J/ψππ vertices are determined by using experimental data based on the branching ratios. In order to estimate the Pc(4380) and Pc(4450) coupling constants, we use the experimental upper limit on the total cross section as a guide for the πN→J/ψN reaction. The background total cross section is the order of 10−4–10−3 nb. In the vicinity of the heavy pentaquark masses, the total cross section reaches about 1 nb
Identification of Kidney Injury Molecule (KIM 1) as a new interactor of polycystin 2 and characterisation of the intracellular domain regarding the ciliary function of KIM 1
No full textDie Gruppe der Polyzystische Nierenerkrankungen wird durch die Dysfunktion ziliär lokalisierter Proteine verursacht. Von besonderer Bedeutung für die ADPKD sind die beiden Polycystine PC 1 und PC 2, beide Proteine sind über ihre ziliär vermittelten mechanosensorische Eigenschaften in den Calciumsignalweg involviert. Polycystin 2 ist ein Calciumkanal und gehört zu der Familie der TRP-Kanäle. Das Zilium nimmt in der wissenschaftlichen Betrachtung der Polycystischen Nierenerkrankung eine zentrale Rolle ein. KIM 1 ist ein ebenfalls ziliär lokalisiertes Protein, welches bei einer ischämischen Schädigung des Nierengewebes eine verstärkte Expression aufweist. Zudem ist KIM 1 auch in zystisch verändertem Nierengewebe nachweisbar. Am Zilium wirkt KIM 1, wie die Polycystine, auf den Calciumsignalweg ein und besitzt chemosensorische Eigenschaften. Die ziliäre Lokalisation von KIM 1 ist essentiell für die mechanosensorischen Eigenschaften dieses Proteins. Mit Hilfe von Trunkationen konnte gezeigt werden, dass die ziliäre Lokalisation von KIM 1 durch eine Sequenz innerhalb der ersten sechs intrazellulären Aminosäuren festgelegt wird. Die Vermutung liegt nahe, dass wie bei anderen ziliären Signalsequenzen auch hier besonders basische, hydrophobe Aminosäuren, in diesem Fall Lysin, Arginin und Histidin entscheidend sein könnte. Zwischen KIM 1 und Polycystin 2 konnte mittels Koimmunopräzipitationen eine Interaktion nachgewiesen werden. Diese Interaktion könnte auf eine direkte Verbindung von KIM 1 zu ADPKD hinweisen. Dies hätte eine Bedeutung für die Diagnostik von akuten, wie auch heriditären Nierenerkrankungen. Des Weiteren könnte KIM 1 zukünftig als Biomarker an Bedeutung gewinnen, da eine abgespaltene Ektodomäne vom KIM 1 im Urin von Patienten mit Nierenparenchymschäden nachgewiesen werden konnte. Zudem existieren Hinweise, dass KIM 1 eine Rolle als Exosomenfänger im renalen Tubulus innehaben könnte. Im Zuge dieser Arbeit gelang die Identifikation eines weiterer Interaktionspartner von KIM 1. KIM 1 zeigte in Koimmunopräzipitationen eine Interaktion mit TRPV4. TRPV4 ist wie Polycystin 2, ein ziliär lokalisierter Kationenkanal, der Familie der TRP-Kanäle. Die Betrachtung dieser Ergebnisse legt die Vermutung nahe, dass KIM 1 über eine spezifische Sequenz Interaktionen mit weiteren TRP Kanälen eingehen könnte.Polycystic Kidney Diseases are caused by a dysfunction of ciliar proteins. The two Polycystins PC 1 and PC 2 are responsible for the autosomal dominant form (ADPKD). Both proteins are through different signal ways involved in calcium signaling. PC 2 is a calcium channel belonging to the family of TRP channels. Proper cilia in renal cells are essential for the function of this organ, in ADPKD the role of cilia is constricted.
KIM 1 is a ciliary protein as well, which is significant up regulated after ischemia. Expression of KIM 1 is also described in cystic degenerated cells. Like Polycystins KIM 1 is involved in calcium signaling, therefore a proper ciliary location seems to be important. With the help of truncations we could show, that the determination of ciliary location of KIM 1 is caused by the first six amino acids. Like in other signal sequences there might be a cluster of hydrophobic, basic amino acids.
Furthermore it was possible to show an interaction between KIM 1 and PC 2 via co immunoprecipitation. This interaction could indicate a direct connection between KIM 1 and ADPKD. This possible connection might have a relevance regarding the diagnostic of polycystic kidney disease.
We could also show another interaction between KIM 1 und TRPV4, another cation channel and member of the TRP channel family. In summary it seems reasonable to assume that there might be a specific signaling sequence for the interaction between KIM°1 and TRP channels.
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