1,721,123 research outputs found
Pyrene-Modified Guanine Cluster Probes Forming DNA/RNA Hybrid Three-Way Junctions for Imaging of Intracellular MicroRNAs
No full textMicroRNAs (miRNAs) regulate gene expression in cells; high levels of expression are associated with various cancers. In this paper, we describe PyA-modified nucleic acid probes that can detect intracellular miRNAs by forming DNA/RNA hybrid three-way junction structures containing a fluorescent scaffold - a so-called G-cluster. This G-cluster featured two mismatched strands, four guanine residues, and one fluorescent adenine residue having a pyrene moiety covalently connected at the 8-position through an acetylene linker. The scaffold underwent a dramatic shift in its emission wavelength when two mismatched strands formed a duplex, similar to the behavior of an adenine pentad system (A-cluster). We applied the G-cluster scaffold in a three-way junction system to probe for miRNAs; its red-shifted fluorescence intensity and stability were greater than those reported previously for A-cluster three-way junction probes. Furthermore, confocal microscopy of cancer cell lines revealed bright fluorescence emissions in response to the miRNAs in the cells. Thus, this system can be applied intracellularly as a potential fluorescent probe for the detection of various biologically important nucleic acids. ?11Nscopu
Detection of AAG repeats through DNA triplex-induced G-cluster formation
No full textThis communication describes a novel method, using DNA triplex-based fluorescent probes, for the detection of AAG repeats. A triplex structure with target DNA AAG repeats stabilizes a (Py)A-modified G-cluster adjacent to the triplex-forming sequence, resulting in a dramatic change in the color of fluorescence from blue to orange.11Nsciescopu
Disassembly of Chromophore-Guided DNA Duplexes through Site-Selective Binding of Coralyne to Pyrene-Modified Adenine Bases
No full textUnder ambient conditions the disassembly of self-duplexes of (Py)A-modified oligodeoxyadenylates (i.e., chromophore-guided DNA assemblies) through intercalative binding of coralyne units to pyrene-modified adenine ((Py)A) bases was carried out. The coralyne units, intercalated site-selectively on the 3 sides of the (Py)A units, are stabilized through - stacking interactions. This competitive intercalation destabilizes the stacking interactions of pairs of (Py)A units, resulting in dissociation of the self-duplexes. This behavior, which is opposite to that of coralyne-induced self-duplex formation of natural oligodeoxyadenylates, was exploited to prepare new types of DNA modules that can be switched between two different duplex states.1100sciescopu
Transcriptome profiling and deciphering the regulatory role of global transcription factor fadR
No full textA new strategy for in silico gene knock-out for strain improvement: Succinate production in E.coli as an example
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Transcriptome analysis of regulatory gene in sulfur containing amino acid biosynthesis, cysBin Escherichia coli
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