121,224 research outputs found
Validation of a Polymerase Chain Reaction technique for Kidd blood group genotyping
No full textThe Kidd blood group antigens, Jkª and Jkᵇ , are two of the
main surface markers which are found on the membrane of red blood
cells. The determination of whether a donor or a recipient has the
Jkª and/or the Jkᵇ antigens is crucially important to have a successful
transfusion without the development of adverse incompatibility-related
reactions. In Malta, routine serological-based tests are applied with
the purpose of differentiating between homozygous and heterozygous
states for the Jk antigens respectively. Although these tests are
highly specific and sensitive, there are particular clinical scenarios
where haemagglutination assays are not suitable for determining the
individual’s Kidd blood group status. Additionally, the alternative
genotyping procedure has never been applied in Malta within the
context of blood grouping. The current study was therefore carried out
to determine whether a molecular-based technique such as Polymerase
Chain Reaction – Restriction Fragment Length Polymorphism analysis
(PCR-RFLP) is a suitable alternative procedure for distinguishing
amongst the three different Kidd phenotypes. After extracting
deoxyribonucleic acid (DNA) from 50 blood samples obtained from
serologically-tested healthy blood donors who expressed at least one of
the Kidd antigens, PCR-RFLP analyses were carried out. The results
of the latter were then compared with those previously obtained with
haemagglutination and a complete match was observed between the
two. Therefore, this PCR-RFLP method was confirmed as a suitable
alternative laboratory technique that can be used to determine
efficiently the Kidd blood group of both donors and recipients, in an
accurate manner without subjectivity as encountered in the case of
haemagglutination. This research further facilitates the introduction of
molecular-based techniques in molecular blood transfusion.peer-reviewe
Kidd, L, 414698
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/397101Surname: KIDD. Given Name(s) or Initials: L. Military Service Number or Last Known Location: 414698. Missing, Wounded and Prisoner of War Enquiry Card Index Number: 55230.234342
Item: [2016.0049.29394] "Kidd, L, 414698
College Plan
No full textCollege plan (perspective). Printed drawing by John L. Kidd. John L. Kidd is/was the architect for Selkirk College
Library Building
No full textLibrary Building (Architect’s conception). Printed drawing by John L. Kidd. John L. Kidd is/was the architect for Selkirk College
West Kootenay Regional College
No full textCollege plan with buildings identified. Printed drawing by John L. Kidd. John L. Kidd is/was the architect for Selkirk College. West Kootenay Regional College former name of Selkirk College
Juanita Kidd Stout Collection
No full textPhotograph of L to R: Rowena Chandler, Juanita Kidd Stout and Mary Chandler Kidd, May 1963
A Comparison of management styles of Japanese manufacturing firms in the UK and in Italy
No full textJuanita Kidd Stout Collection
No full textPhotograph of L to R: front to back, Nellie Dunlap, Justice Juanita Kidd Stout, and Beulah Walters, May 1989
Jim Gray and John L. Kidd with the Winning Design
No full textJim Gray is/was Chairman, Selkirk College Council. John L. Kidd is/was the architect for Selkirk College.
Photo taken by the Castlegar News of the submissions to the architectural competition for a design for Selkirk College
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