100,920 research outputs found

    Validation of a Polymerase Chain Reaction technique for Kidd blood group genotyping

    No full text
    The Kidd blood group antigens, Jkª and Jkᵇ , are two of the main surface markers which are found on the membrane of red blood cells. The determination of whether a donor or a recipient has the Jkª and/or the Jkᵇ antigens is crucially important to have a successful transfusion without the development of adverse incompatibility-related reactions. In Malta, routine serological-based tests are applied with the purpose of differentiating between homozygous and heterozygous states for the Jk antigens respectively. Although these tests are highly specific and sensitive, there are particular clinical scenarios where haemagglutination assays are not suitable for determining the individual’s Kidd blood group status. Additionally, the alternative genotyping procedure has never been applied in Malta within the context of blood grouping. The current study was therefore carried out to determine whether a molecular-based technique such as Polymerase Chain Reaction – Restriction Fragment Length Polymorphism analysis (PCR-RFLP) is a suitable alternative procedure for distinguishing amongst the three different Kidd phenotypes. After extracting deoxyribonucleic acid (DNA) from 50 blood samples obtained from serologically-tested healthy blood donors who expressed at least one of the Kidd antigens, PCR-RFLP analyses were carried out. The results of the latter were then compared with those previously obtained with haemagglutination and a complete match was observed between the two. Therefore, this PCR-RFLP method was confirmed as a suitable alternative laboratory technique that can be used to determine efficiently the Kidd blood group of both donors and recipients, in an accurate manner without subjectivity as encountered in the case of haemagglutination. This research further facilitates the introduction of molecular-based techniques in molecular blood transfusion.peer-reviewe

    Population genetics characteristics of a 90 locus panel of microhaplotypes

    No full text
    Microhaplotype genotype data for 556 individuals (with anonymized identifiers) from 16 population samples.[1] This research receiveded funding support in part from the National Institute of Justice of the U.S. Department of Justice, grant number 2018-75-CX-0041 awarded to Kenneth K. Kidd, Ph.D. and in part from the National Institute of Health of the United States, grant number R01-HD102537 awarded to Curt Scharfe, M.D., Ph.D. [2] The many samples of populations studied in the Kidd laboratory since 1985 have all been collected with informed consent under a general Yale protocol (HIC#8711001387) that was reviewed and approved by the NIGMS and CEPH. One third of the samples in the CEPH-HGDP collection came from Kidd lab population samples. (NIGMS stands for National Institute of General Medical Sciences within the U.S. National Institute of Health. French acronym CEPH translates as the Center for the Study of Human Polymorphisms. HGDP indicates the Human Genome Diversity Project.

    Joshua Davis: Author of Spare Parts

    No full text
    Citation: K-State First (2016). Joshua Davis: Author of Spare Parts [Flier]. Manhattan, Kansas: K-State First.Flyer advertising Joshua Davis's author talk at Kansas State University

    Letter from J. K. Kidd to William F. M. Arny

    No full text
    Letter dated August 22, 1864 from J. K. Kidd, Fort Union, New Mexico, to William Frederick Milton Arny, Secretary of the Territory of New Mexico, Santa Fe, asking for an appointment in one of the New Mexican Regiments. Kidd was in the Eleventh Regiment, Missouri Calvary at Fort Union. HL introduction page overlaid by document. Letter in English, handwritten, 1pp/fr

    kidd

    No full text
    kid n[One type of slingshot] is made of an alder stalk, with rubber attached to the stalk and the "kidd". The "kidd" is usually made of leather, preferably the tongue of an old boot.DNE-citJH 10/73Used IUsed I1Used I[see 'rubber-gun]; kid-board, kid's eyeChecked by Raji Sreeni on Mon 06 Jul 201

    Erratum to: Is Sensory Loss an Understudied Risk Factor for Frailty? A Systematic Review and Meta-analysis

    No full text
    In the article “Is Sensory Loss an Understudied Risk Factor for Frailty? A Systematic Review and Meta-analysis,” an author was missing. Ana Maseda should be listed as the 11th author. The correct author list is: Benjamin Kye Jyn Tan, Ryan Eyn Kidd Man, Alfred Tau Liang Gan, Eva K Fenwick, Varshini Varadaraj, Bonnielin K Swenor, Preeti Gupta, Tien Yin Wong, Caterina Trevisan, Laura Lorenzo-López, Ana Maseda, José Carlos Millán-Calenti, Carla Helena Augustin Schwanke, Ann Liljas, Soham Al Snih, Yasuharu Tokuda, Ecosse Luc Lamoureux. This error has been corrected

    Steven Johnson Author Talk Poster

    No full text
    K-State Book NetworkA poster advertising an author talk by Steven Johnson at Kansas State University on September 3, 2014. Steven Johnson's book "The Ghost Map" was the 2014-2015 common book

    MF2782

    No full text
    Tandalayo Kidd & Carolyn Snyder, Kansans move into health, Kansas State University, July 2007

    MF2783

    No full text
    Tandalayo Kidd & Carolyn Snyder, Kansans move into health: leader's guide, Kansas State University, July 2007
    corecore