167,495 research outputs found
Validation of a Polymerase Chain Reaction technique for Kidd blood group genotyping
No full textThe Kidd blood group antigens, Jkª and Jkᵇ , are two of the
main surface markers which are found on the membrane of red blood
cells. The determination of whether a donor or a recipient has the
Jkª and/or the Jkᵇ antigens is crucially important to have a successful
transfusion without the development of adverse incompatibility-related
reactions. In Malta, routine serological-based tests are applied with
the purpose of differentiating between homozygous and heterozygous
states for the Jk antigens respectively. Although these tests are
highly specific and sensitive, there are particular clinical scenarios
where haemagglutination assays are not suitable for determining the
individual’s Kidd blood group status. Additionally, the alternative
genotyping procedure has never been applied in Malta within the
context of blood grouping. The current study was therefore carried out
to determine whether a molecular-based technique such as Polymerase
Chain Reaction – Restriction Fragment Length Polymorphism analysis
(PCR-RFLP) is a suitable alternative procedure for distinguishing
amongst the three different Kidd phenotypes. After extracting
deoxyribonucleic acid (DNA) from 50 blood samples obtained from
serologically-tested healthy blood donors who expressed at least one of
the Kidd antigens, PCR-RFLP analyses were carried out. The results
of the latter were then compared with those previously obtained with
haemagglutination and a complete match was observed between the
two. Therefore, this PCR-RFLP method was confirmed as a suitable
alternative laboratory technique that can be used to determine
efficiently the Kidd blood group of both donors and recipients, in an
accurate manner without subjectivity as encountered in the case of
haemagglutination. This research further facilitates the introduction of
molecular-based techniques in molecular blood transfusion.peer-reviewe
Rodney J. Kidd
No full textRodney J. Kidd directs Texas Interscholastic League, world\u27s largest. Kidd is shown holding a telephone.https://mavmatrix.uta.edu/specialcollections_startelegram1950s/10870/thumbnail.jp
Thomas J. Kidd
No full textEngraving of Thomas J. Kidd who was general manager of the Farmers and Ginners Cotton Oil Company. He was director of the First National Bank and the Kyser Manufacturing Company of Birmingha
Letter from J. K. Kidd to William F. M. Arny
No full textLetter dated August 22, 1864 from J. K. Kidd, Fort Union, New Mexico, to William Frederick Milton Arny, Secretary of the Territory of New Mexico, Santa Fe, asking for an appointment in one of the New Mexican Regiments. Kidd was in the Eleventh Regiment, Missouri Calvary at Fort Union. HL introduction page overlaid by document. Letter in English, handwritten, 1pp/fr
Leon Kidd
No full textLeon Kidd, son of Arnold and Alice Kidd of Vernal, graduated form Weber State University
Elynne Kidd
No full textElynne Kidd is the daughter of Arnold Ray and Alice Kidd. She married Percy Stewart Jr
Wayne Kidd
No full textWayne Kidd, son of Dee and Estell Kidd, earned his Duty to God Award in the Ashley Ward of the Church of Jesus Christ of Latter-day Saints
Serena Kidd
No full textSerena Kidd, daughter of Leon and Terrie Kidd, was selected to be a contestant in the Utah All American Miss Pageant
At the Captain Kidd
No full textAt the Captain Kidd. Left to right: Ron Vale, Shinya Inoue, J. Woodland Hastings, Andrew Szent-Gyorgyi, unknown.Photograph
Arline Kidd
No full textArline Kidd is pictured her freshman year at Uintah High School. She born to Alexander Carson and Flora Belle Kidd on October 31, 1923. She married Lester Martin in 1942. She died February 4, 2007
- …
