13,753 research outputs found
The Effects of Partial or Total Replacement of Maize Silage by Panicum maximum Silage in Lactating Cows Rations on Ruminal Fermentation, Digestibility and Methane Production
No full textFIVE TOTAL mixed rations (TMR) were formulated to evaluate the influence of partial total replacement of maize silage (MS) with Panicum maximum silage (PS) on digestibility, rumen fermentation and milk yield of cows. The experimental rations were TMR contained 100% Maize silage (MS, T1); TMR with 75% MS + 25% PS (T2); TMR with 50% MS + 50% PS (T 3); TMR with 25% MS + 75% PS (T 4) and TMR with 100% PS (R5). Milk yield was performed on fifty Friesian cows (10 cows per treatment) for three months. Rumen fermentation and gas production were determined with Barki rams. The results cleared that CP content of both silages were close together, but ash and NDF were higher for PS but lactic and acetic acids were high for MS. Gas production (GP) was lower for PS on both 24 and 48 h than MS. Meanwhile, both GP for soluble (a), insoluble (b) and the cumulative GP (y) were less with PS than MS, as well as DMD, OMD and SCFA. Rumen fermentation data showed higher NH3-N, less TVFA's and acetic with the increase of PS in the rations, while the control, R2 and R3 had more acetic compared to other rations. Rations contained 25 and 50 % of PS had more milk and 4% FCM yields, while the lowest milk yield was obtained by incorporating 100% PS in ration (R5). It could be advisable to PS replacing MS in ration up to 50% without any adverse effect on milk production
Ms. Courtney Chartier, RWWL AUC, August 2011
No full textThis video is a conversation with Ms. Courtney Chartier. Ms. Chartier talks about her work on the "New Georgia Encyclopedia" and "Online Voter Education Project." Andrea Jackson, AUC Woodruff Library, is the interviewer
Ms. Neely Terrell, RWWL AUC, March 2012
No full textThis video is a conversation with Ms. Neely Terrell. Ms. Terrell talks about her book, "Super Singles Activate". Anthony Kinsey and Jahnesta Horney, AUC Woodruff Library, are the interviewers
Ms. Felesha Love, Spelman College, January 2016
No full textThis video is a conversation with Felesha Love. Ms. Love talks about her book, "Brave Leap to Freedom: Integrating Mind, Body, and Spirit to Cultivate Healthy Relationships". Jordan Moore, AUC Woodruff Library, is the interviewer
LC-API/MS in Drug Metabolism and Pharmacokinetic Studies
No full textThe use of API interfaces with quadrupole mass spectrometers has been shown to give rise to good sensitivity, selectivity, and robustness for the interfacing of LC to MS. Since their introduction in the 1990s the technique has rapidly become widespread, but at the outset of this research programme, there were still a number of problems associated with it, particularly when dealing with complex sample matrices. The aim of this research programme was to study illustrative examples of the kinds of problems associated with the analysis of biological samples using LC-API-MS in an attempt to arrive at strategies which could be employed to eliminate, or at least compensate for, the problems.
Commonly reported problems include the occurrence of matrix effects - a change in response of the target analyte(s) as a result of the presence in the samples of co /late eluting interferences. An investigation which compared ESI with APCI ionisation illustrated a significant drawback in the accepted methodology for the elimination of matrix effects.
Optimal LC conditions for a number of assays may use non-MS-friendly mobile phases. A simple and convenient solution to this problem was found to be the post column addition of organic modifier, which reproducibly and reliably enhanced sensitivity. This approach was initially used for a range of dihydropyridine calcium channel blockers and was subsequently applied to a range of chiral compounds from different therapeutic groups to illustrate that this was applicable as a generic technique for increasing sensitivity (typically by around an order of magnitude) in low organic mobile phases.
Strategies to develop and validate methods for the determination of endogenous analytes in a biological fluid were investigated. This involved the use of a surrogate matrix, to develop a method for the determination of endogenous testosterone in human serum and the use of non-matrix calibration standards for the successful development and validation of a method for the analysis of indolyl 3 acryloylglycine (IAG) in human urine.
As a result of observations suggesting promotion of ionisation of deltamethrin in liver tissue sample extracts, it was postulated that this was due to the presence of high concentrations of surfactants. After confirming the effect, a series of systematic investigations were performed to attempt to understand the mechanism to be able to utilise this as a general method for the enhancement of signal with low sensitivity analytes. It was found that the type of surfactant and concentration used was directly associated with an increased (or decreased) response.
Although there remain a number of problems associated with the use of LC-API-MS, the work undertaken for this thesis has successfully demonstrated a number of techniques that can be applied to overcome these problems. Knowledge of the nature of the sample undergoing analysis, the required analytical conditions, and where required careful application of one of the techniques described will ensure that a robust method can be readily developed
Intrathecal IgM Synthesis Is Associated with Spinal Cord Manifestation and Neuronal Injury in Early MS
No full textOBJECTIVE
Intrathecal Immunoglobulin M synthesis (IgMIntrathecal Fraction (IF) + ) and spinal MRI lesions are both strong independent predictors of higher disease activity and severity in multiple sclerosis (MS). We investigated whether IgMIF + is associated with spinal cord manifestation and higher neuroaxonal damage in early MS.
METHODS
In 122 patients with a first demyelinating event associations between 1.) spinal versus (vs) non-spinal clinical syndrome 2.) spinal vs cerebral T2-weighted (T2w) and 3.) contrast-enhancing (CE) lesion counts with IgGIF + (vs IgGIF - ) or IgMIF + (vs IgMIF - ) were investigated by logistic regression adjusted for age and sex, respectively. For serum neurofilament light chain (sNfL) analysis patients were categorized for presence or absence of oligoclonal IgG bands (OCGB), IgGIF and IgMIF (>0% vs 0%, respectively): 1) OCGB- /IgGIF - /IgMIF - ; 2) OCGB+ /IgGIF - /IgMIF - ; 3) OCGB+ /IgGIF + /IgMIF - ; and 4) OCGB+ /IgGIF + /IgMIF + . Associations between categories 2) to 4) vs category 1 with sNfL concentrations were analyzed by robust linear regression, adjusted for sex and MRI parameters.
RESULTS
Patients with a spinal syndrome had a 8.36-fold higher odds of IgMIF + (95%CI 3.03-23.03; p <0.01). Each spinal T2w lesion (odds Ratio 1.39; 1.02-1.90; p = 0.037) and CE lesion (OR 2.73; 1.22-6.09; p = 0.014) was associated with an increased risk of IgMIF + (but not of IgGIF + ); this was not the case for cerebral lesions. OCGB+ /IgGIF + /IgMIF + category patients showed highest sNfL levels (estimate:1.80; 0.55-3.06; p <0.01).
INTERPRETATION
Intrathecal IgM synthesis is strongly associated with spinal manifestation and independently more pronounced neuroaxonal injury in early MS, suggesting a distinct clinical phenotype and pathophysiology. This article is protected by copyright. All rights reserved
Phytochemical composition and biological activity of extracts from Lebanese medicinal plants
No full textThe Mediterranean area especially the eastern part including Lebanon, is rich in term of plant biodiversity, including high valuable medicinal plants that are largely used by people as herbal medicine, these plants represent an important source of plant-derived bioactive compounds, besides, there is a need to characterize these compounds, evaluate their bioactivity and clarify scientifically the use of these plant as traditional and alternative medicine, lack of these information may limit their use, by contrast, studying the compositions and bioactivity of these plants may improve their use, discovery of new natural bioactive compounds and finally formulate new plant-based nutraceutical and therapeutically agents. In this context, the aim of my PhD thesis, and as a part of our ongoing program in the medicinal plants filed, was the characterization of a pool of phytochemical compounds with a special focus on polyphenols isolated from different medicinal plants from the Lebanese flora, using analytical tools (as described later in the chapters) including high-performance liquid chromatography (HPLC) coupled with different detectors such as UV-visible and electrospray ioniziation (ESI) Mass spectrometry (MS), following by the study of their biological activities using different in vitro cellular well-established model for several chronic diseases. The plants that I choose for my study have a significant value in the traditional medicine in Lebanon, where they are employed as a food but also in folk medicine to treat/prevent many illnesses. I employed different cellular models of oxidative stress and related chronic diseases (NAFLD, inflammation and cancer)
Étude sur le patois de Valbonnais
No full textA lexical and morphologic description of Valbonnais dialect. A 319-page PhD dissertation under the direction of Prof. Antonin DURAFFOUR (Univ. Stendhal, Grenoble, France, 1943)Description lexicale et morphologique du patois de Valbonnais sous la forme d'un manuscrit de 319 pages.Thèse sous la direction du Prof. Antonin DURAFFOUR (Univ. Stendhal, Grenoble, 1943
Fragmentation of Cannabidiol Bio-derivatives by HPLC Analysis, Soft Ionization Tandem Mass Spectrometry (HPLC MS/MS)
No full textThe study aims to analyze the fragmentation patterns of cannabidiol (CBD) bio-derivatives using High-Performance Liquid Chromatography coupled with Tandem Mass Spectrometry (HPLC-MS/MS). By employing soft ionization techniques (ESI and APCI), the research focuses on identifying structural integrity and cleavage pathways of three specific CBD derivatives. This approach enhances understanding of their breakdown mechanisms, which is crucial for optimizing their pharmacokinetic properties and therapeutic potential. The study underscores the role of structural modifications, like Mannich-type reactions, in improving CBD’s solubility, stability, and efficacy for medical applications.BSc/BAchemcial engineerin
Radar Chart of Citation topic Meso, source: Formulated by author using MS Excel (2016).
No full textRadar Chart of Citation topic Meso, source: Formulated by author using MS Excel (2016).</p
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