2 research outputs found
The genesis of cerebellar GABAergic neurons: fate potential and specification mechanisms
No full textThe variety of neuronal phenotypes that populate the cerebellum derives from progenitors that proliferate in two germinal neuroepithelia: the ventricular zone generates GABAergic neurons, whereas the rhombic lip is the origin of glutamatergic types. Progenitors of the ventricular zone produce GABAergic projection neurons (Purkinje cells and nucleo-olivary neurons) at the onset of cerebellar neurogenesis. Later on, however, these progenitors migrate into the prospective white matter, where they continue to divide up to postnatal development and generate different categories of inhibitory interneurons, according to precise spatio-temporal schedules. Projection neurons derive from discrete progenitor pools located in distinct microdomains of the ventricular zone, whereas interneurons originate from a single population of precursors, distinguished by the expression of the transcription factor Pax-2. Heterotopic/heterochronic transplantation experiments indicate that interneuron progenitors maintain full developmental potentialities up to the end of cerebellar development and acquire mature phenotypes under the influence of environmental cues present in the prospective white matter. Furthermore, the final fate choice occurs in postmitotic cells, rather than dividing progenitors. Extracerebellar cells grafted to the postnatal cerebellum are not responsive to local neurogenic cues and fail to adopt clear cerebellar identities. On the other hand, cerebellar cells grafted to extracerebellar regions retain typical phenotypes of cerebellar GABAergic interneurons, but acquire specific traits under the influence of local cues. These findings indicate that interneuron progenitors are multipotent and sensitive to spatio-temporally patterned environmental signals that regulate the genesis of different categories of interneurons, in precise quantities and at defined times and places
Coarse woody debris dynamics in a secondary Atlantic Forest fragment in Brazil
No full textThe Atlantic Forest fragments have suffered from the impacts of climate change, resulting in the increased production of Coarse Woody Debris (CWD), which needs to be evaluated in space and time to generate accurate estimates of carbon accumulation. Thus, the goals of this study were: i) to quantify the CWD volume, necromass, carbon stock and annual increment of carbon (AIcarb) over a period of 4 years; and ii) to select the optimal combination of climatic, topographic, edaphic and intrinsic forest variables to accurately predict AIcarb, using machine learning and multivariate analysis. The CWD volume, necromass and carbon stock increased between 2017 and 2020. The AIcarb was 1.09 MgC.haThe presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
