198,274 research outputs found
Kern
No full text1. Te majd kézenfogsz és hazavezetsz / Presser + 2. A szomorú bárzongorista dala / Karácsony - Presser, Nagy Feró + 3. Ez van / Dusán + 4. A tükör / Karácsony - Dusán, Kern + 5. Halálos tangó / Presser - Dusán + 6. Annyira mégse rossz / Zorán - Dusán, Kern + 7. Pesten születtem / Presser - Dusán + 8. Zenés-táncos nőnapi ünnepség a Guvátinál nyereségrészesedéssel / Presser - A jelenetet írta: Kern, Dusán, Presser + 9. Szerelmünk tárgytalan / Pavel Danek - Dusán + 10. Az élet szép / Dusán + 11. Én nem megyek moziba többé / Presser - Dusán + 12. Mikor leszek szemüveges? / Bornai - Kern + 13. Szép kezű lány / Horváth A. - Kern + 14. Esik / Novák - Kern + 15. 10 perces szerelem / Máté P. - Kern - Horváth A. + 16. Látlak Amerika / Másik J. - Kern + 17. Pincér-rock / Heilig G. - Kern, Horváth A. + 18. Lövölde Tér / Másik J. - Kern, Horváth A. + 19. Leszállás / Laár A. - Ker
Kern
No full text1. Te majd kézenfogsz és hazavezetsz / Presser + 2. A szomorú bárzongorista dala / Karácsony - Presser, Nagy Feró + 3. Ez van / Dusán + 4. A tükör / Karácsony - Dusán, Kern + 5. Halálos tangó / Presser - Dusán + 6. Annyira mégse rossz / Zorán - Dusán, Kern + 7. Pesten születtem / Presser - Dusán + 8. Zenés-táncos nőnapi ünnepség a Guvátinál nyereségrészesedéssel / Presser - A jelenetet írta: Kern, Dusán, Presser + 9. Szerelmünk tárgytalan / Pavel Danek - Dusán + 10. Az élet szép / Dusán + 11. Én nem megyek moziba többé / Presser - Dusán + 12. Mikor leszek szemüveges? / Bornai - Kern + 13. Szép kezű lány / Horváth A. - Kern + 14. Esik / Novák - Kern + 15. 10 perces szerelem / Máté P. - Kern - Horváth A. + 16. Látlak Amerika / Másik J. - Kern + 17. Pincér-rock / Heilig G. - Kern, Horváth A. + 18. Lövölde Tér / Másik J. - Kern, Horváth A. + 19. Leszállás / Laár A. - Ker
De divinis personis
No full textin ... academia Dilingana anno salutis M.DC.XI die XXVI. Septembris proposita, praeside P. Georgio Kern ... respondente ... Iacobo Algeier ...TiteleinfassungDiss. Univ. Dillingen, 161
De ultimo fine hominis vero ac divinitus praefinito
No full textin ... academia Dilingana anno salutis M.DC.X die XXVI. Ianuarii publice proposita, praeside P. Georgio Kern ... respondente ... Wolfgango Aigenman, Monacensi, S.D.N. alumno ...TiteleinfassungDiss. Univ. Dillingen, 161
De gratia divina iustificante
No full textin ... academia Dilingana, anno salutis M.DC.XI, die [7] Februarii proposita, praeside P. Georgio Kern ... respondente ... F. Bernardo Han, Weingartensi, ord. S. BenedictiTiteleinfassungPaginierungsfehler: anstelle von Seite 38 ist Seite 28 aufgedrucktDiss. Univ. Dillingen, 161
Optomotor course control in flies with largely asymmetric visual input
No full textKern R, Egelhaaf M. Optomotor course control in flies with largely asymmetric visual input. Journal of Comparative Physiology A. 2000;186(1):45-55
Introduction of functional CAR-T activity tests in vitro
No full textCelična terapija s CAR-T se je v preteklih letih izkazala za eno najobetavnejših novih terapij za zdravljenje malignih obolenj. Zaradi uporabe bolniku lastnih (avtolognih) celic, ki predstavljajo spremenljiv dejavnik, se pojavlja potreba po in vitro testiranju aktivnosti celic CAR-T pred vnosom v pacienta. V magistrski nalogi smo s pomočjo elektroporacije v celično linijo humanih limfocitov T CD4+ vnesli receptor CAR anti-CD19 in reporterski protein GFP, nato smo dvojno pozitivne celice ločili s pomočjo FACS in tako dobili homogeno celično kulturo. Celice CAR-T smo izpostavili antigenu CD19. Gojili smo jih v kokulturi s celično linijo humanih limfocitov B, ki izražajo ta antigen. Po 48 urah kokultivacije smo v supernatantu celične kokulture s pomočjo posrednega testa ELISA določali koncentracijo citokinov IL-2, TNF-alfa in IFN-gama. Citokini v supernatanu kokulture so pokazatelj aktivacije celic CAR-T z antigenom CD-19. Celice smo prešteli s pomočjo Bürker – Türkove števne komore pod fluorescentnim in pod svetlobnim mikroskopom, da bi ugotovili, kako se je spremenilo število celic in razmerje med efektorskimi (celice CAR-T, ki zeleno fluorescirajo) in tarčnimi celicami (limfociti B, ki ne fluorescirajo). Z optimalnimi pogoji elektroporacije smo dosegli, da je 22,1 % živih celic izražalo CAR in GFP. V 48 urah kokultivacije se razmerje med efektorskimi in tarčnimi celicami ni spremenilo, prav tako se ni spremenila končna skupna koncentracija celic. Statistično značilno razliko v izločanju citokinov IL-2 in TNF-alfa smo opazili pri razmerju efektorskih in tarčnih celic 1:1. Izločanja IFN-gama z našim testom nismo zaznali. Pokazali smo, da je kokultivacija celic CAR-T skupaj s specifičnim antigenom povzročila izločanje citokinov, ki jih lahko kvantitativno določimo z indirektnim testom ELISA in s tem posredno ovrednotimo pričakovano učinkovitost terapije.CAR-T cell therapy is one of the most promising new therapies for treatment of malignancies. Because of the use of autologous cells, which are a variable factor, there is a need for in vitro testing of CAR-T cell activity prior to aplication to patient. In the thesis, electroporation was used to insert anti-CD19 CAR receptor and reporter protein GFP into CD4+ human lymphocyte cell line. Next, double positive cells were sorted using fluorescence-assisted cell sorting to acquire a homogenous cell culture. CAR-T cells were then exposed to the antigen CD19 present on human B-cell line. We cocultured both cell lines for 48 hours. Using an indirect ELISA test, we then determined the concentration of cytokines IL-2, TNF-alpha, and IFN-gamma in the cell culture supernatant. Cytokines in the coculture supernatant indicate that activation of CAR-T cells via CD-19 antigen was successful. Cells were counted using Bürker – Türk counting chamber to determine how the number of cells in the culture and the ratio between effector (fluorescent CAR-T cells) and target cells (B-cells) has changed. With optimised electroporation parameters we accomplished expression of CAR and GFP proteins in 22,1 % of live cells. In the 48 hours of cocultivation the ratio between effector and target cells did not change. The density of cell culture did not differ between test and control. Statistically significant difference in cytokine IL-2 and TNF-alpha secretion was observed in effector and target cell ratio of 1:1. Secretion of IFN-gamma cytokine was not detected. We showed that cocultivating CAR-T cells with their specific antigen causes activation of the cells and secretion of cytokines which can be quantitatively measured using indirect ELISA test. The results can be used to indirectly predict the efficacy of cell therapy
Therapy with modified T-cells that express chimeric receptor for tumor antigens
No full textCAR-T celična terapija je ena od novejših in uspešnejših metod za zdravljenje raka. Temelji na opremljanju pacientu avtolognih celic T z receptorji CAR, ki so sposobni prepoznati tumorske antigene in sprožiti odziv limfocitov T. Receptorji CAR so fuzijski proteini sestavljeni iz značilnih domen: vezavne domene, ekstracelularne povezovalne domene, transmembranske domene, signalizacijske domene in kostimulatorne domene. Vsaka od njih ima specifično funkcijo in vpliva na delovanje receptorja CAR in CAR-T celične terapije. Produkcija celic CAR-T poteka v več korakih. Najprej pacientu odvzamejo limfocite T, nato jih aktivirajo in transformirajo, jih razmnožijo in vrnejo v pacienta v obliki infuzije. Celice se nato še naprej razmnožujejo v pacientu in vršijo svojo funkcijo napadanja in uničevanja tumorskih celic. Kljub začetnim velikim uspehom terapije na področju zdravljenja levkemij, ima terapija svoje resne stranske učinke. Glavna problema terapije sta sindrom prekomernega izločanja citokinov in toksičnost zaradi napada na zdrave celice, ki izražajo tumorski antigen. Pojavljajo se mnoge rešitve teh problemov. Glavne so uporaba samomorilskih mehanizmov, pri katerih se celice CAR-T ob prisotnosti induktorja uničijo, in uporaba kombinacij receptorjev CAR z bolj specifično ločevanje tumorskih od zdravih celic.CAR-T cell therapy is one of the newest and most successful methods for treating cancer. The therapy is based on transducing CAR receptors into patient\u27s autologous T cells. CAR receptors are fusion proteins capable of recognising tumour antigens and activating lymphocyte T response. CAR receptors comprise of five typical domains: antigen-binding domain, extracellular spacer, transmembrane domain, signaling domain and costimulatory domain. Each of them has a specific function and affects how the CAR receptor and CAR-T cell therapy performs. Production of CAR-T cells is done in multiple steps. First, patient’s allogenic lymphocytes T are taken from his blood, then they are activated and transformed with CAR receptor gene, expanded and returned into the patient’s blood in a form of an infusion. After the infusion, the cells continue to proliferate in the patient and perform their function of attacking and killing tumour cells. Alongside the initial big success of the therapy for leukemias, there are some severe side effects of the therapy that need to be addressed. The biggest issues are cytokine release syndrome and on-target off tumour toxicities. Various solutions for the problems have arisen. The main two strategies are the use of suicide mechanisms that induce apoptosis in CAR-T cells when inductor molecule is administered, and the use of a combination of CAR receptors that is capable of distinguishing between heathy and cancerous cells
Rezension zu: P. Glotzt: Im Kern verrottet?
No full textHuber L. Rezension zu: P. Glotzt: Im Kern verrottet? Das Hochschulwesen. 1998;46(1):58f
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