162,461 research outputs found
Plasticity of gastro-intestinal vagal afferent endings
No full textAbstract not availableStephen J. Kentish, Amanda J.Pag
Nesfatin-1 modulates murine gastric vagal afferent mechanosensitivity in a nutritional state dependent manner
No full textAbstract not availableStephen J. Kentish, Hui Li, Claudine L. Frisby, Amanda J. Pag
Circadian variation in gastric vagal afferent mechanosensitivity
No full textFood intake is coordinated to cellular metabolism by clock gene expression with a master clock in the suprachiasmatic nucleus synchronized by light exposure. Gastric vagal afferents play a role in regulating food intake, but it is unknown whether they exhibit circadian variation in their mechanosensitivity. We aimed to determine whether gastric vagal afferents express clock genes and whether their response to mechanical stimuli oscillates throughout the light/dark cycle. Nodose ganglia were collected from 8-week-old female C57BL/6 mice every 3 h starting at lights off (1800 h) to quantify Bmal1, Per1, Per2, and Nr1d1 mRNA by qRT-PCR. Additionally in vitro single-fiber recordings of gastric vagal mechanoreceptors were taken at all time points. Per1, Per2, Bmal1, and Nr1d1 mRNA is expressed in the nodose ganglia and levels oscillated over a 24 h period. In mice fed ad libitum, gastric content was 3 times higher at 0000 h and 0300 h than 1200 h. The response of tension receptors to 3 g stretch was reduced by up to 70% at 2100 h, 0000 h, and 0300 h compared with 1200 h. Gastric mucosal receptor response to stroking with a 50 mg von Frey hair was 3 times greater at 1200 h and 1500 h than the response at 0000 h. Similar findings were obtained in mice fasted for 6 h or maintained in darkness for 3 d before study. Therefore, these changes do not result from food intake or the light/dark cycle. Thus, gastric vagal mechanoreceptors display circadian rhythm, which may act to control food intake differentially at different times of the day.Stephen J. Kentish, Claudine L. Frisby, David J. Kennaway, Gary A. Wittert, and Amanda J. Pag
High fat diet induced changes in gastric vagal afferent response to adiponectin
No full textNo abstract availableStephen J. Kentish, Kyle Ratcliff, Hui Li, Gary A. Wittert, Amanda J. Pag
Apelin modulates murine gastric vagal afferent mechanosensitivity
No full textAvailable online 28 June 2018Abstract not availableHui Li, Stephen J. Kentish, Gary A. Wittert, Amanda J. Pag
The role of gastrointestinal vagal afferent fibres in obesity
No full textTopical ReviewGastrointestinal (GI) vagal afferents are a key mediatory of food intake. Through a balance of responses to chemical and mechanical stimuli food intake can be tightly controlled via the ascending satiety signals initiated in the GI tract. However, vagal responses to both mechanical and chemical stimuli are modified in diet-induced obesity (DIO). Much of the research to date whilst in relatively isolated/controlled circumstances indicates a shift between a balance of orexigenic and anorexigenic vagal signals to blunted anorexigenic and potentiated orexigenic capacity. Although the mechanism responsible for the DIO shift in GI vagal afferent signalling is unknown, one possible contributing factor is the gut microbiota. Nevertheless, whatever the mechanism, the observed changes in gastrointestinal vagal afferent signalling may underlie the pathophysiological changes in food consumption that are pivotal for the development and maintenance of obesity.Stephen J. Kentish and Amanda J. Pag
[Report to Chief J. E. Curry, by an unknown author #1]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
[Report to Chief J. E. Curry, by an unknown author #2]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
Neuropeptide W modulation of gastric vagal afferent mechanosensitivity: impact of age and sex
No full textAbstract not availableHui Li, Claudine L. Frisby, Tracey A. O’Donnell, Stephen J. Kentish, Gary A. Wittert, Amanda J. Pag
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