1,721,071 research outputs found
Dimerization of thyroglobulin in the endoplasmic reticulum
No full textThe kinetics of dimerization of a newly synthesized thyroglobulin (Tg), the precursor protein in the manufacture of thyroid hormone, was investigated in the endoplasmic reticulum of thyrocytes FRTL-5 cell line. The folded monomeric Tg was first detectable in a conformationally unstable form, from the examination of lysates of pulse labeled cultured thyrocytes by denaturing and nondenaturing gel electrophoresis by 15 min after biosynthesis. The first dimeric Tg was formed by 30 min after; the monomer declined and the dimer progressively increased, and 40 min after remarkable dimeric Tg form was found. Finally, dimerization was complete at 60 min after.open
The importance and global trends of biological resources: the introduction of the KCTC and fungal resources
No full textIn 1985, the Korean Collection for Type Cultures (KCTC), a former organization of the Korea Research Institute of Bioscience and Biotechnology Biological Resource Center (KBRC), was officially approved by the Ministry of Science and Technology (MOST) as a gene bank node and became a member of the World Federation for Culture Collections (WFCC). The KBRC was also designated as an International Depository Authority (IDA) under Budapest Treaty in 1990. As a national bio-infra for biological resources, the main functions of the KBRC are 1) collection, preservation and distribution of biological resources, 2) research and development of core technologies for valuable bioresources, and 3) construction of local and international network of biological resources and information. As major activities in 2007, about 1,300 type and reference strains including bacteria, actinomycetes, yeasts, filamentous fungi, anaerobes, cell lines and patent strains were newly acquired and about 4,000 strains were distributed to academia, industries and research institutes. The KBRC published 49 papers regarding biological resources and described 18 new microbial species. Especially, a big progress was made in several aspects: establishment of the back-up depository system for over 3,300 patent strains, development of the barcode system for computerized and centralized management of biological resources, and structural remodeling. The KBRC is going to promote tighter networking among domestic and international culture collections, to strengthen the national depository system for bioproducts from research, and to emphasize research and development related to the collection and preservation of valuable biological resources. Consequently, the KBRC will expand its roles not only as a national infrastructure for life science and biotechnology but also as a fundamental basis of many industries in an era of bio-economy in the 21st century.open
Assessment of random amplified polymorphic DNA (RAPD) for determining the origin of Youngia koidzuminana kitamura(compositae)
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The significance of ITS-RFLPs and coenzyme Q system in determining taxonomic relationships among Candida species
No full textThe taxonomic relationship of 32 species in the genus Candida Berkhout was examined by characterizing the restriction patterns of nuclear internal transcribed spacers and coenzyme Q types. A matrix of 116 characters was generated from 32 species and seven restriction endonucleases, and phenetically analyzed. Unweighted pair-group arithmetic average (UPGMA) analysis was used to construct the phenograms. The 32 species fell into five major groups corresponding with their genetic relationships. The medically important Candida species clustered together, except C. glabrata. Almost identical restriction patterns were observed between C. hydrocarbofumarica and C. blankii, C. stellatoidea, and C. albicans, and C. steatolytica, and C. inositophila due to synonymous or opposite mating type relationships. The members in each group did not show the same coenzyme Q types, whereas the closely clustered members in each group exhibited the same coenzyme Q types. The results of this study, analyzing ITS-RFLPs and the coenzyme Q type, provided an insight into the various genetic origins of Candida species as well as their genetic relationships.open
A research on the diversity and enzyme productivity of halophilic microorganisms isolated from the soil around the ranch for the exploration of fermentation strains
No full textThe diversity and characterization of microorganisms isolated from the soil around several ranches in Korea were confirmed in this study. To isolate halophilic microorganisms, the marine agar medium was basically used and cultivated at 37oC for several days. After single colony isolation, a total of 116 pure colonies were isolated and phylogenetic analysis was carried out based on the result of 16S rDNA sequencing, indicating that isolated strains were divided into 4 phyla, 23 families, 30 genera and 51 species. To confirm whether isolated strain can be a candidate for the fermentation of diverse food ingredients, amylase, lipase, and protease enzyme assays were performed individually, showing that 92 strains possessed at least one enzyme activity. Especially 4 strains, identified to Jonesia quinghaiensis (isolate name: JSF 19-2), Halomonas alkaliantarctica (isolate name: JSF 21), Bacillus velezensis (isolate name: NWFY-36), and Staphylococcus capitis subsp. urealyticus (isolate name: MSY-5), showed all enzyme activity tested. Moreover, 17 strains showed the ability for auxin production. This result indicated that isolated strains have shown the possibility of the application for the food and feed industries. Therefore, this study has contributed to securing domestic biological resources and the improvement of hydrolytic enzyme activity by using isolated strains.
Lutibacter oricola sp. Nov., a marine bacterium isolated from seawater
No full textA bacterial strain, UDC377T, was isolated from seawater samples collected at Seo-do on the coast of Dokdo island in the East Sea, and was subjected to taxonomic study using a polyphasic approach. Strain UDC377T was pale-yellow, Gram-staining-negative, non-motile, rod-shaped and aerobic. The strain grew optimally at 25？28 6C, in the presence of 2% (w/v) NaCl and at pH 7.0？7.5. Strain UDC377T produced carotenoid pigments; however, it did not produce flexirubin-type pigments. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain UDC377T clustered with members of the genus Lutibacter and appeared most closely related to Lutibacter agarilyticus KYW566T (96.0% 16S rRNA gene sequence similarity) followed by L. aestuarii MA-My1T (95.0%), L. litoralis CL-TF09T (94.9%), L. maritimus S7-2T(94.1%) and L. flavus IMCC1507T (94.0%). The DNA G+C content of strain UDC377T was 30.8 mol%. Strain UDC377T contained MK-6 as the predominant menaquinone, iso-C15: 0 3-OH, iso-C15 : 0 and iso-C16 : 0 3-OH as the major fatty acids, and phosphatidylethanolamine, two unknown aminolipids and six unknown lipids as the major polar lipids. Based on phenotypic properties and phylogenetic data presented, strain UDC377T is considered to represent a nov.l sp.cies of the genus Lutibacter, for which the name Lutibacter oricola sp. nov. is proposed. The type strain is UDC377T (5DSM 24956T5KCTC 23668T).open
Umboniibacter roseus sp. nov., isolated from coastal seawater
No full textA Gram-reaction-negative, non-motile, strictly aerobic darkness orange pigmented and rod-shaped bacterial isolate, designated strain 14-121-B13T, was isolated from surface seawater off the coast of the South Sea at Namhae-gun, Republic of Korea. Cells were catalase- and oxidase-positive and required NaCl for growth. Strain 14-121-B13T grew optimally at 30 °C, in the presence of 2% (w/v) NaCl and at pH7.5-8.0. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain 14-121-B13T clustered with the type strain of Umboniibacter marinipuniceus, with which it exhibited 96.7% sequence similarity. The DNA G+C content was 48.9 mol%. The major cellular fatty acids were summed feature 3 (C16:1 ω7c and/or C16:1 ω6с) and C16:0. The major respiratory quinone was ubiquinone Q-7 and the polar lipids detected in strain 14-121-B13T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipids, unidentified aminophospholipids and unidentified lipids. Based on phenotypic, chemotaxonomic and phylogenetic data presented, strain 14-121-B13T is considered to represent a novel species of the genus, Umboniibacter for which the name Umboniibacter roseus sp. nov. is proposed. The type strain is 14-121-B13T (=DSM 29882T =KCTC 42467T).open
Mesonia maritima sp. nov., isolated from seawater of the South Sea of Korea
No full textA yellow-pigmented bacterial strain, originally designated 15-S14-6T, was isolated from the southern coastal waters of Korea and was subjected to taxonomic study using a polyphasic approach. Strain 15-S14-6T was Gram-staining-negative, flagellated, rod-shaped and aerobic. The strain grew optimally at 25-30？°C, in the presence of 2% (w/v) NaCl and at pH 7.5-8.0. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that 15-S14-6T represented a member of the genus Mesonia and exhibited the highest sequence similarity (98.0%) with Mesonia aquimarina IMCC1021T, followed by Mesonia mobilis KMM 6059T (96.7%) and Mesonia phycicola MDSW-25T (96.1%). DNA-DNA relatedness between 15-S14-6T and M. aquimarina IMCC1021T was 11.2±1.6% (12.3±0.3% in a reciprocal experiment). Strain 15-S14-6T had a DNA G+C content of 34.4？mol% and had MK-6 as the predominant menaquinone. Furthermore, iso-C15:0, iso-C17:0 3-OH and summed feature 3 were identified as the major fatty acids. Phosphatidylethanolamine, two unknown aminolipids, an unknown phospholipid and four other unknown lipids were determined to be the major polar lipids. Based on phenotypic properties and phylogenetic data presented here, 15-S14-6T is considered to represent a novel species of the genus Mesonia, for which the name Mesonia maritimus sp. nov. is proposed. The type strain is 15-S14-6T (= KCCM 43197T, NCAIM B.02633T, CGMCC 1.16020T).
A study of the diversity and profile for enzyme production of aerobically cultured halophilic microorganisms from the various solar salterns
No full textThis research confirmed the diversity and characterization of halophilic microorganisms isolated from the various solar salterns, collected on the inside and outside of the country. To isolate strains, the marine agar medium was basically used and cultivated at 37oC for several days aerobically. After single colony isolation, a totally of 230 pure colonies were isolated and phylogenetic analysis was carried out based on the result of 16S rDNA sequencing, indicating that isolated strains were divided into 4 phyla, 12 families, 27 genera and 64 species. Firmicutes phylum, the main phyletic group, comprised 89.6% with 3 families, 17 genera and 52 species of Bacillaceae, Staphylococcaceae and Carnobacteriaceae. To confirm whether isolated strain can produce industrially useful enzyme or not, amylase, lipase, and protease enzyme assays were performed individually, showing that 177 strains possessed at least one enzyme activity. Especially 17 strains showed all enzyme activity tested. This result indicated that isolated strains have shown the possibility of the industrial application. Therefore, this study has contributed to securing domestic genetic resources and the expansion of scientific knowledge of the halophilic microorganisms community in solar salterns.
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