5,648 research outputs found
Recovery of a Medieval Brucella melitensis genome using shotgun metagenomics
No full textABSTRACT Shotgun metagenomics provides a powerful assumption-free approach to the recovery of pathogen genomes from contemporary and historical material. We sequenced the metagenome of a calcified nodule from the skeleton of a 14th-century middle-aged male excavated from the medieval Sardinian settlement of Geridu. We obtained 6.5-fold coverage of a Brucella melitensis genome. Sequence reads from this genome showed signatures typical of ancient or aged DNA. Despite the relatively low coverage, we were able to use information from single-nucleotide polymorphisms to place the medieval pathogen genome within a clade of B. melitensis strains that included the well-studied Ether strain and two other recent Italian isolates. We confirmed this placement using information from deletions and IS711 insertions. We conclude that metagenomics stands ready to document past and present infections, shedding light on the emergence, evolution, and spread of microbial pathogens
Self-compression of 4.9 µm pulses to sub-40 fs with 2 mJ energy in Zinc Sulfide
No full textNonlinear self-compression of few-cycle multi-mJ pulses at 4.9 µm in ZnS is presented. 80 fs input pulses are compressed to 37 fs with 2.1 mJ energy at a 1 kHz repetition rate. © 2024 The Author(s
Correction to: Chamoun et al., Bacterial pathogenesis and interleukin-17: interconnecting mechanisms of immune regulation, host genetics, and microbial virulence that influence severity of infection
No full textChamoun MN, Blumenthal A, Sullivan MJ, Schembri MA, Ulett GC. 2018. Bacterial pathogenesis and interleukin-17: interconnecting mechanisms of immune regulation, host genetics, and microbial virulence that influence severity of infection. Critical Reviews in Microbiology. https://doi.org/10.1080/1040841X.2018.1426556.
When the above article was first published online, the below three corrections were missed.
The author ‘Antje Blumenthal’ was wrongly affiliated to the affiliation “cSchool of Chemistry and Molecular Biosciences, and Australian Infectious Disease Research Centre, The University of Queensland, Brisbane, Australia”. Now this affiliation has been removed for this author.
The affiliation ‘bTranslational Research Institute, The University of Queensland Diamantina Institute, Woolloongabba, Australia’ of the author ‘Antje Blumenthal’ should read ‘bThe University of Queensland Diamantina Institute, Translational Research Institute, Brisbane, Australia’.
In Table 3, the sentence ‘Benefit of manipulating IL-17 levels to improve immunization strategies M. tuberculosis’ should read “Benefit of manipulating IL-17 levels to improve immunization strategies against M. tuberculosis”.No Full Tex
Generation of 22-mJ, 2.0-ps Pulses from a 1-kHz Ho:YLF Regenerative Chirped Pulse Amplifier
No full textWe report a CW-pumped Ho:YLF regenerative amplifier (RA) delivering pulses with 22.5-mJ energy and 2.0-ps duration at 1 kHz. The RA emitting at 2051 nm is broadband-seeded and implemented in a chirped pulse amplification system. © 2024 The Author(s
Racial differences in the overexpression of epidermal growth factor type II receptor (HER2/neu): a major prognostic indicator in uterine serous papillary cancer.
No full textOBJECTIVE: A difference in survival rates between black and white patients with cancer of the corpus uteri is well established. This study was conducted to determine whether the overexpression of HER2/neu oncogene is associated with poor outcome in uterine serous papillary endometrial cancer, which is a highly aggressive variant of endometrial cancer, and whether a racial difference in the frequency of HER2/neu overexpression may contribute to the disparity in endometrial cancer survival.
STUDY DESIGN: Immunohistochemical evaluation was used to examine HER2/neu expression in paraffin blocks from 27 women with stage IA to IV uterine serous papillary endometrial cancer. Univariable analysis was performed and followed by multivariable analysis with Cox's proportional hazard model to evaluate whether HER2/neu expression was associated with poor outcome in uterine serous papillary endometrial cancer.
RESULTS: Black patients tended to be younger (P = .02) and have higher HER2/neu expression than white patients (trend P = .02). Seven of 10 black patients (70%) showed heavy (3+) expression, compared with 4 of 17 white patients (24%; P = .04). The association of heavy HER2/neu expression with race persisted after age was controlled through stratification (P = .05). Earlier deaths from uterine serous papillary endometrial cancer were seen among heavy HER2/neu expressers (P = .002), black patients (P = .04), and patients < or = 65 years old (P = .04). However, multivariate Cox regression showed that short survival was associated significantly with heavy HER2/neu expression (P = .02) but not with age (P = .07) or race (P = .35), which indicates that HER2/neu expression accounted for much of the race disparity in survival in this patient population.
CONCLUSION: Overexpression of HER2/neu in uterine serous papillary endometrial cancer is an independent variable that is associated with poor outcome, occurs more frequently in black women, and may contribute to racial disparity in survival. HER2/neu expression may guide clinical treatment of patients with uterine serous papillary endometrial cancer and may have implications for the implementation of novel treatment strategies
Pure-rotational 1D-CARS spatiotemporal thermometry with a single regenerative amplifier system
No full textWe report spatiotemporal pure-rotational coherent anti-Stokes Raman spectroscopy (CARS) in a one-dimensional imaging arrangement obtained with a single ultrafast regenerative amplifier system. The femtosecond pump/Stokes photon pairs, used for impulsive excitation, are delivered by an external compressor operating on a ∼35% beam split of the uncompressed amplifier output (2.5 mJ/pulse). The picosecond 1.2 mJ probe pulse is produced via the second-harmonic bandwidth compression (SHBC) of the ∼65% remainder of the amplifier output (4.5 mJ/pulse), which originates from the internal compressor. The two pump/Stokes and probe pulses are spatially, temporally, and repetition-wise correlated at the measurement, and the signal generation plane is relayed by a wide-field coherent imaging spectrometer onto the detector plane, which is refreshed at the same repetition rate as the ultrafast regenerative amplifier system. We demonstrate 1 kHz cinematographic 1D-CARS gas-phase thermometry across an unstable premixed methane/air flame-front, achieved with a single-shot precision <1% and accuracy <3%, 1.4 mm field of view, and an excellent <20 µm line-spread function.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Flight Performance and Propulsio
Project: Establishing an accounting outsourcing company "MJ Grāmatvedība" Ltd
No full textProjekta ideja ir izveidot grāmatvedības ārpakalpojumu uzņēmumu SIA „MJ Grāmatvedība”, kurš sniegtu pilna vai daļēja cikla grāmatvedības pakalpojumus, sākot ar pirmreizējo dokumentu apstrādi un beidzot ar visu nepieciešamo atskaišu sagatavošanu, kā arī konsultāciju sniegšanu. Projekta mērķis ir ne tikai sniegt grāmatvedības pakalpojumus, bet arī analizēt uzņēmumu darbību un izstrādāt ieteikumus darbības uzlabošanai. Darba autore uzskata, ka nozares pārstāvjiem ir būtiski jāpierāda sava nozīme un to kādam jābūt mūsdienu grāmatvedim. Grāmatvedis nav tikai persona, kura izdara konkrētus darbus, nodos atskaites, tas ir cilvēks, kuram labi jāpārzina nodokļu sistēma, finanšu vadīšana, jāspēj sniegt padomus uzņēmuma finanšu vadības uzlabošanai, kā arī jāspēj pielāgoties tehnoloģiju radītajām izmaiņām. Maģistra darbs apjoms ir uz 105 lappusēm, kas sastāv no 7 nodaļām, 17 pielikumiem, 22 tabulām un 7 attēliem. Darba izstrādē izmantoti 70 informācijas avoti.The general project idea is to establish the accountancy firm MJ Accountancy Ltd., which would provide full or half-cycle accountancy services including the processing of primary documentation, reports’ preparation, and providing of the accountancy consultations. The aim of the project is not only to provide accountancy consultations but also to deal with firms activity analysis and developing suggestions on their activity improvement. The author of the project supports the idea that the field representatives must constantly sustain the status of the accountant’s profession in current times. The accountant is not only the person who executes one’s regular duties and submits reports, but also the person who is competent in the field of taxes, financial management, able to advise on improvements in financial management, and above all able to adapt to the technological changes. The volume of the project is 105 pages, which includes 7 chapters, 17 appendices, 22 tables, 7 figures. 70 sources of information were used in master’s thesis
Remodelling of cerebrospinal fluid lipoproteins after subarachnoid hemorrhage
No full textLipoprotein particles (Lps) in normal human cerebrospinal fluid (CSF) are distinct from those found in plasma and include unique apolipoprotein E (apoE indicates protein; APOE, gene) containing lipoproteins rarely seen in human plasma. Less favourable neurological recovery after subarachnoid hemorrhage (SAH) has been observed in patients who posses the APOE 4 allele raising the possibility that apoE influences neuronal survival after brain injury. We analysed Lps from control and SAH CSF testing the hypotheses that following brain injury CSF Lps undergo remodelling and apoE containing Lps are selectively depleted from brain injury CSF. Lipoproteins were fractionated using CSF from six control pools and six patients with SAH on a sepharose 6HR 10/30 size exclusion column. Fractions were assayed for total cholesterol (TC), free cholesterol (FC), phospholipid, triglyceride (TG), apoE, apolipoprotein B (apoB), and apolipoprotein AI (apoAI). Compared to control CSF there were significant (P<0.05) increases in TC, FC, TG, and apoAI in SAH CSF. Plasma sized apoB-containing lipoproteins and a very small apoAI-containing Lps were identified in the SAH CSF, which were not present in controls. However, despite the release of plasma lipoproteins into the subarachnoid space, there was no significant increase in CSF apoE. These data provide novel indirect evidence suggesting that after SAH CSF Lps undergo remodelling and apoE containing Lps are selectively reduced in brain injury CSF. The remodelling of CSF Lps and selective reduction of apoE containing lipoproteins may reflect an important response of the human brain to injury
Initial state and transition-state solvation effects in the cobaltotungstate oxidation of iodide in binary aqueous solvent mixtures
No full textPT: J; CR: AMJAD Z, 1977, CAN J CHEM, V55, P3581 BAKER LCW, 1956, J AM CHEM SOC, V78, P4503 BECK MT, 1968, COORDIN CHEM REV, V3, P91 BLANDAMER MJ, UNPUB CAN J CHEM BLANDAMER MJ, 1978, J CHEM SOC CHEM COMM, P963 BLANDAMER MJ, 1979, PURE APPL CHEM, V51, P2087 BLANDAMER MJ, 1980, COORDIN CHEM REV, V31, P93 BLANDAMER MJ, 1980, J CHEM SOC DA, P1 BLANDAMER MJ, 1980, J CHEM SOC DA, P2442 BRODOVITCH JC, UNPUB BURGESS J, 1968, J CHEM SOC A, P2571 BURGESS J, 1970, J CHEM SOC A, P2111 BURGESS J, 1970, J CHEM SOC A, P2351 BURGESS J, 1972, INORGANIC REACTION M, V2, P127 BURGESS J, 1973, J CHEM SOC A, P825 BURGESS J, 1974, INORGANIC REACTION M, V3, P142 BURGESS J, 1977, INORGANIC REACTION M, V5, P158 BURGESS J, 1979, INORGANIC REACTION M, V6, P168 COX BG, 1974, ANN REP CHEM SOC A, V71, P249 COX BG, 1979, J CHEM SOC F1, V75, P1780 COX BG, 1979, J CHEM SOC FARAD T 1, V75, P86 DELIGNY CL, 1965, RECL TRAV CHIM PAY B, V84, P81 ELLIS KJ, 1973, J CHEM SOC DA, P1533 GRUNWALD E, 1948, J AM CHEM SOC, V70, P846 KANEMAQUIRE LAP, 1975, J CHEM SOC DA, P1890 KEPERT DL, 1978, J CHEM SOC DA, P137 MARCUS RA, 1968, J PHYS CHEM-US, V72, P891 PELIZZETTI E, 1976, INORG CHEM, V15, P2898 SUBHANI MS, 1978, REV ROUMAINE CHIM, V23, P719 UDOVENKO VV, 1977, RUSS J INORG CHEM, V22, P168 WELLS CF, 1973, J CHEM SOC FARAD T 1, V69, P984 WELLS PR, 1968, LINEAR FREE ENERGY R, CH4; NR: 32; TC: 14; J9: TRANSIT METAL CHEM; PG: 4; GA: NG073Source type: Electronic(1
An extension of the weighted sum of gray gases non-gray gas radiation model to a two phase mixture of non-gray gas with particles
No full textA great deal of efforts has been exercised to date to accurately model the non-gray behavior of the gases. Among others, the weighted sum of gray gases model (WSGGM), which replaces the non-gray gas behavior by an equivalent finite number of gray gases, is a simplified model yielding reasonable results. However, a discussion on the weighting factors required for an estimation of radiation in a mixture of non-gray gas/gray particulate is not yet established for WSGGM, since they are dependent on the particle number density, particle size distribution: local temperature and partial pressure. Consequently, the relation between the weighting factors used in the WSGGM for a mixture of non-gray gas and gray particles with scattering in the thermal non-equilibrium has been discussed here, which has not been done before to the author's best knowledge. Weighting factors for the particles, of which temperature is different from that of the gas, were evaluated analytically for the WSGGM. The results were, then, validated for the problem of isothermal gas containing soot particulates between two parallel slab walls. For further application, the approach derived here was implemented to examine the non-gray radiative effects of the two phase mixture in an axisymmetric cylinder by changing such various parameters as the particle temperature, non-gray gas composition and particle concentration. The effects of thermal non-equilibrium in a mixture of gas and particles were also discussed in parallel with scattering effects by particles. Parametric study showed that a variation in the gas concentration yielded a noticeable change in the radiative heat transfer when the suspended particle temperature was different from the gas temperature. New contribution of this study consisted in an extension of applicability of the WSGGM non-gray model to two phase radiation. (C) 2000 Elsevier Science Ltd. All rights reserved.The authors wish to acknowledge the financial support of the Korea Research Foundation made in the program year of 1998 under the contract 1998-018-E00016
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