4,092 research outputs found
Genetic Analysis of cagA and vacA Genes in Helicobacter Pylori Isolates and Their Relationship with Gastroduodenal Diseases in the West of Iran
No full textBackground: Helicobacter pylori have different virulence factors which are associated with several gastroduodenal diseases; however, this association is variable in different geographical regions. Data of genotypes of Iranian H. pylori isolates are few. Objectives: The aim of the current study was to investigate the cagA/vacA genotypes of Helicobacter pylori isolates and determine the relationship between these genotypes with respect to different gastric disorders in patients of Chaharmahalo Bakhtiarian. Materials and Methods: In this cross-sectional study, gastric biopsies were taken from 200 patients with gastrodoudenal diseases. Histopathological features were recognized by specialist. The samples were subjected to PCR for detection and identification of ureC, cagA and vacA genes. Results: The frequency of the vacA genotypes, sa1/m1, s1a/m1b, s1a/m2, s1b/m1a, s1b/m1b, s1b/m2, s1c/m1a, s1c/m1b, s1c/m2, s2/m1a, s2/m1b and s2/ m2 were 27(6.6%), 8(4.3%), 45(28.04%), 7(3.7%), 5(2.5%), 10 (6.1%), 12 (7.4%), 4 (2.5%), 18(11%), 6(3.7%), 0 and 22(13.5%) respectively. The cagA gene was detected in 92% of strains. Based on our findings, it seemed that cagPAI and vacA s1 genotypes were associated with some gastric disorders in patients with H. pylori. In this region, the isolates carrying s1a/m2 were the most prevalent. Conclusions: We found considerable relationship between s1a/m1a, s1a/m2, s2/m2 and s1c/m1a and some gastric disorders. Further studies about the role of H. pylori virulence factors and gastric disorders were recommended. © 2013, Iranian Red Crescent Medical Journal
Mathematics, Logic, and their Philosophies:Essays in Honour of Mohammad Ardeshir
No full textThis volume is a collection of essays in honour of Professor Mohammad Ardeshir. It examines topics which, in one way or another, are connected to the various aspects of his multidisciplinary research interests. Based on this criterion, the book is divided into three general categories. The first category includes papers on non-classical logics, including intuitionistic logic, constructive logic, basic logic, and substructural logic. The second category is made up of papers discussing issues in the contemporary philosophy of mathematics and logic. The third category contains papers on Avicenna’s logic and philosophy.Mohammad Ardeshir is a full professor of mathematical logic at the Department of Mathematical Sciences, Sharif University of Technology, Tehran, Iran, where he has taught generations of students for around a quarter century. Mohammad Ardeshir is known in the first place for his prominent works in basic logic and constructive mathematics. His areas of interest are however much broader and include topics in intuitionistic philosophy of mathematics and Arabic philosophy of logic and mathematics. In addition to numerous research articles in leading international journals, Ardeshir is the author of a highly praised Persian textbook in mathematical logic. Partly through his writings and translations, the school of mathematical intuitionism was introduced to the Iranian academic community
Mathematics, Logic, and their Philosophies:Essays in Honour of Mohammad Ardeshir
No full textThis volume is a collection of essays in honour of Professor Mohammad Ardeshir. It examines topics which, in one way or another, are connected to the various aspects of his multidisciplinary research interests. Based on this criterion, the book is divided into three general categories. The first category includes papers on non-classical logics, including intuitionistic logic, constructive logic, basic logic, and substructural logic. The second category is made up of papers discussing issues in the contemporary philosophy of mathematics and logic. The third category contains papers on Avicenna’s logic and philosophy.Mohammad Ardeshir is a full professor of mathematical logic at the Department of Mathematical Sciences, Sharif University of Technology, Tehran, Iran, where he has taught generations of students for around a quarter century. Mohammad Ardeshir is known in the first place for his prominent works in basic logic and constructive mathematics. His areas of interest are however much broader and include topics in intuitionistic philosophy of mathematics and Arabic philosophy of logic and mathematics. In addition to numerous research articles in leading international journals, Ardeshir is the author of a highly praised Persian textbook in mathematical logic. Partly through his writings and translations, the school of mathematical intuitionism was introduced to the Iranian academic community
Molecular epidemiology of ESBLs genes and multi-drug resistance in diarrheagenic Escherichia Coli strains isolated from adults in Iran
No full textResistance to oxyimino cephalosporins antibiotics in Enterobacteriaceae is primarily done by the extended spectrum beta-lactamases (ESBLs). Clear identification of risk factors for ESBLs-producing infections is necessary. Therefore, efficient strategies can be developed to decrease outbreak of these infections. The aim of this study was to determine the antibacterial susceptibility and ESBLs pattern of diarrhogenic Escherichia coli (E. coli) strains isolated from adult patients. In the present study, diarrheogenic E. coli strains were isolated from 54 patients from the University of Medical Sciences hospitals in Shiraz. Antimicrobial susceptibility testing was done by disk diffusion method by CLSI criteria. The presence of bla(TEM), bla(SHV) and bla(CTX-M) genes was investigated by PCR using designated primers. The prevalence of ESBLs-producer E. coli strains was 12.96%. Antimicrobial resistance testing showed a high resistance to cefexime, trimethoprim-sulfamethoxazole, ampicillin and penicillin. Overall, beta-lactamase genes were identified in 52 (96.30%) isolates which were identified as 45 (83.33%) bla(TEM), 17 (31.48%) bla(SHV) and 11 (20.37%) bla(CTX-M). ESBLs-producer E. coli is very prevalent in diarrheogenic strains isolated from adult patients. Also, this study clearly showed that the blaT(EM) gene for ESBLs-producer E. coli was widespread in Iran
PP-005 Clarithromycin resistance assessment in Helicobacter pylori isolates by using 23S rRNA gene molecular markers
No full textBackground: H. pylori is a relatively fastidious and microaerophilic microorganism and therefore standard phenotypic susceptibility tests, even in the hands of experts, are slow and can take at least 10 14 days. Molecular based diagnostic assays by using molecular markers for resistance detection offer an attractive alternative approach to obtain susceptibilities to antibiotics with greater accuracy and speed, and the possibility of a same day result. The aim of this study is the assessment of clarithromycin resistance by
using molecular markers. Methods: This cross-sectional descriptive study was performed on 200 gastric biopsy specimens which were obtained from patients undergoing upper gastrointestinal tract endoscopy in Hajar hospital of Shahrekord, by using TaqMan real-time PCR. Initially, H. pylori strains were identified by RUT and PCR. Then, by this regard that accumulation of mutations associated with resistance to clarithromycin were in the region between nucleotides 2142 2144 of 23S rRNA gene, the first probe was designed
to be able the distinguish between sensitive and resistant strains. Finally four probes were designed that each be able to identify only one mutation associated with a particular level of clarithromycin resistance. Results: Out of 200 samples, 164 (82%) were H. pylori positive. Overall, clarithromycin susceptible strains were detected in 105 (64.02%) patients and clarithromycin resistance were detected in 59 (35.98%) which were identified as 4 (2.44%) A2144G, 26 (15.85%) A2143G, 15 (9.15%) A2143C and 20 (12.19%) A2142G point mutations. Purely resistant strains were detected in 38 (23.17%), while heteroresistant were found in the remaining 16 (9.76%) cases. Genotype of 5 (8.47%) strains was not detected. This data was confirmed by PCR-RFLP technique. Conclusion: Results showed that Real-time PCR assay in combination with molecular markers has high accuracy to simultaneously identify H. pylori and clarithromycin resistance types directly in gastric biopsy specimens in short time
Multiplex PCR assay for rapid determination of bla(TEM), bla(SHV) and bla(CTX-M) genes in diarrheagenic Escherichia coli isolated from Iran, Shiraz
No full textIntroduction: The emergence of Escherichia coli that produce extended spectrum b-lactamases (ESBLs) and are
multidrug resistant poses antibiotic management problems. ESBLs are cephalosporinases that confer resistance to a wide variety of oxyimino cephalosporins and create serious therapeutic problems. The objective of this study was to determine the blaTEM, blaSHV and blaCTX-M Genes of diarrheagenic E. coli. Methods: We conducted a cross sectional-descriptive study on 150 E. coli strains, isolated from patients who had diarrhea at 3 major hospitals in Shiraz from April to November 2010. Initially, E. coli strains isolated by
biochemical analysis. Finally, prevalence of blaTEM, blaSHV and blaCTX-M were determined by multiplex PCR assay. Statistical analyses were performed using the c2 test. Pvalues less than 0.05 were taken to indicate statistical significance. Results: Overall, ESBL-related bla genes were detected in 143 (95.33%) isolates which were identified as 79 (52.66%) TEM, 7 (4.66%) SHV, 4 (2.66%) CTX-M, 12 (8%) TEM/SHV, 16 (10.66%) TEM/CTX-M, 7 (4.66%) SHV/CTX-M and 18 (12%) TEM/SHV/CTX-M. Statistical analysis revealed that more relationships between blaSHV and age 48 60 (p = 0.009) and blaCTX-M age 12 17 (p = 0.009). Conclusion: ESBLs in E. coli has increased in the last few years and increase in cephalosporin resistance is mainly due to an increase in the proportion of isolates carrying the blaTEM gene in Shiraz. ESBL-producing E. coli infections have a significant impact on several important clinical outcomes, and efforts to control outbreaks of infection with ESBLproducing E. coli should emphasize judicious use of all antibiotics as well as barrier precautions to reduce spread
The study of the oipA and dupA genes in Helicobacter pylori strains and their relationship with different gastroduodenal diseases
No full textAim: The purpose of this investigation was to determine the oipA and dupA genes of Helicobacter pylori isolates from west of Iran; Chaharmahalo Bakhtiyari region and find their relationship with the severity of the gastroduodenal diseases. Background: Helicobacter pylori is an organism responsible for many gastroduodenal diseases. Many studies suggest that genetic diversity in H. pylori virulence factors such as oipA and dupA genes is high among isolates of different geographic regions and may cause more severe diseases. Patients and methods: In this cross-sectional study, gastric biopsy specimens were taken from 150 patients suffering from gastroduodenal diseases. The presence of ureC, dupA and oipA genes was tested by polymerase chain reaction (PCR). Results: Overall, 123 (82%) H. pylori strains were isolated from 150 specimens. dupA gene was detected in 41 (33.33%) H.pylori-positive specimens. There was a reverse correlation between this gene and gastric cancer. The oipA gene was found in 88 (71.54%) samples and statistically there was no association between this gene and gastric disorders. As statistical analyses revealed, the presence of the dupA was more common in isolates with the oipA negative. Conclusion: Based on our findings, the presence of dupA gene can be considered as a marker for the onset of severe diseases. However, the oipA gene cannot be regarded for prediction of gastroenterology diseases. Meanwhile, extended molecular epidemiology researches in other populations are recommended
PP-081 Quantitation of bacteria in gastric biopsy specimen from patients with gastrointestinal disorders: relationship between counts and clinical features
No full textBackground: H. pylori is the only bacteria that infect humans gastric for which endoscopy examination is routinely recommended. The risk of development of gastrointestinal disorders (GIDs) in the presence of H. pylori infection depends on a variety of factors including bacterial, host, and environmental ones that mostly relate to the pattern of bacterial load. The aim of this study is to evaluate the relationship between H. pylori disease associated clinical features and bacterial count. Methods: A prospective study of the concentrations of bacteria in the gastric and their relationship to clinical features was conducted with 200 H. pylori suspected patients with GIDs. Initially, clinical status was evaluated and recorded by questionnaire. Then, RUT and 16SrRNA PCR were performed for identification of H. pylori. Finally, gastric biopsy specimens were analyzed for bacterial count by using specific primers and probe for
23SrRNA and using Taq-Man real-time PCR technology. Statistical analyses were performed using the c2 test. Pvalues less than 0.05 were taken to indicate statistical significance. Results: Of whom 200 samples, 164 (82%) had confirmed H. pylori positive. For 164 patients with GIDs, the count of H. pylori in gastric biopsies were a value of 104 to 1012 CFU/ml. The results revealed that more relationships between acid reflux and 104 CFU/ml (p = 0.006) and vomiting and 104 CFU/ml (p = 0.046).
Conclusion: This study has demonstrated a relationship
between bacterial load and clinical features in patients
with GIDs. In conclusion, GIDs are similar to many other
bacterial infections in that bacterial burden in the host is
related to clinical features. Further studies are required
to determine whether this is a function of the host, the
infecting strain, or a combination of the two
Mathematics, Logic, and their Philosophies:Essays in Honour of Mohammad Ardeshir
No full textThis volume is a collection of essays in honour of Professor Mohammad Ardeshir. It examines topics which, in one way or another, are connected to the various aspects of his multidisciplinary research interests. Based on this criterion, the book is divided into three general categories. The first category includes papers on non-classical logics, including intuitionistic logic, constructive logic, basic logic, and substructural logic. The second category is made up of papers discussing issues in the contemporary philosophy of mathematics and logic. The third category contains papers on Avicenna’s logic and philosophy.Mohammad Ardeshir is a full professor of mathematical logic at the Department of Mathematical Sciences, Sharif University of Technology, Tehran, Iran, where he has taught generations of students for around a quarter century. Mohammad Ardeshir is known in the first place for his prominent works in basic logic and constructive mathematics. His areas of interest are however much broader and include topics in intuitionistic philosophy of mathematics and Arabic philosophy of logic and mathematics. In addition to numerous research articles in leading international journals, Ardeshir is the author of a highly praised Persian textbook in mathematical logic. Partly through his writings and translations, the school of mathematical intuitionism was introduced to the Iranian academic community
Mathematics, Logic, and their Philosophies:Essays in Honour of Mohammad Ardeshir
No full textThis volume is a collection of essays in honour of Professor Mohammad Ardeshir. It examines topics which, in one way or another, are connected to the various aspects of his multidisciplinary research interests. Based on this criterion, the book is divided into three general categories. The first category includes papers on non-classical logics, including intuitionistic logic, constructive logic, basic logic, and substructural logic. The second category is made up of papers discussing issues in the contemporary philosophy of mathematics and logic. The third category contains papers on Avicenna’s logic and philosophy.Mohammad Ardeshir is a full professor of mathematical logic at the Department of Mathematical Sciences, Sharif University of Technology, Tehran, Iran, where he has taught generations of students for around a quarter century. Mohammad Ardeshir is known in the first place for his prominent works in basic logic and constructive mathematics. His areas of interest are however much broader and include topics in intuitionistic philosophy of mathematics and Arabic philosophy of logic and mathematics. In addition to numerous research articles in leading international journals, Ardeshir is the author of a highly praised Persian textbook in mathematical logic. Partly through his writings and translations, the school of mathematical intuitionism was introduced to the Iranian academic community
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