89 research outputs found
Nurse Autonomy Pain Control and Discharge from Recovery
No full text Beverley Colwill, the author of this article, was faced with the question, ‘Is it always necessary for patients to remain in the recovery room for 30 minutes following their last intravenous bolus dose of morphine?’ To try to find an answer, she carried out the literature search which is reported here. </jats:p
sj-jpeg-2-cjk-10.1177_20543581231224127 – Supplemental material for Humoral Response Following 3 Doses of mRNA COVID-19 Vaccines in Patients With Non-Dialysis-Dependent CKD: An Observational Study
No full textSupplemental material, sj-jpeg-2-cjk-10.1177_20543581231224127 for Humoral Response Following 3 Doses of mRNA COVID-19 Vaccines in Patients With Non-Dialysis-Dependent CKD: An Observational Study by Omosomi Enilama, Kevin Yau, Lee Er, Mohammad Atiquzzaman, Matthew J. Oliver, Marc G. Romney, Jerome A. Leis, Kento T. Abe, Freda Qi, Karen Colwill, Anne-Claude Gingras, Michelle A. Hladunewich and Adeera Levin in Canadian Journal of Kidney Health and Disease</p
sj-docx-3-cjk-10.1177_20543581231224127 – Supplemental material for Humoral Response Following 3 Doses of mRNA COVID-19 Vaccines in Patients With Non-Dialysis-Dependent CKD: An Observational Study
No full textSupplemental material, sj-docx-3-cjk-10.1177_20543581231224127 for Humoral Response Following 3 Doses of mRNA COVID-19 Vaccines in Patients With Non-Dialysis-Dependent CKD: An Observational Study by Omosomi Enilama, Kevin Yau, Lee Er, Mohammad Atiquzzaman, Matthew J. Oliver, Marc G. Romney, Jerome A. Leis, Kento T. Abe, Freda Qi, Karen Colwill, Anne-Claude Gingras, Michelle A. Hladunewich and Adeera Levin in Canadian Journal of Kidney Health and Disease</p
sj-jpeg-1-cjk-10.1177_20543581231224127 – Supplemental material for Humoral Response Following 3 Doses of mRNA COVID-19 Vaccines in Patients With Non-Dialysis-Dependent CKD: An Observational Study
No full textSupplemental material, sj-jpeg-1-cjk-10.1177_20543581231224127 for Humoral Response Following 3 Doses of mRNA COVID-19 Vaccines in Patients With Non-Dialysis-Dependent CKD: An Observational Study by Omosomi Enilama, Kevin Yau, Lee Er, Mohammad Atiquzzaman, Matthew J. Oliver, Marc G. Romney, Jerome A. Leis, Kento T. Abe, Freda Qi, Karen Colwill, Anne-Claude Gingras, Michelle A. Hladunewich and Adeera Levin in Canadian Journal of Kidney Health and Disease</p
sj-docx-1-cjk-10.1177_20543581231160511 – Supplemental material for Determining the Longitudinal Serologic Response to COVID-19 Vaccination in the Chronic Kidney Disease Population: A Clinical Research Protocol
No full textSupplemental material, sj-docx-1-cjk-10.1177_20543581231160511 for Determining the Longitudinal Serologic Response to COVID-19 Vaccination in the Chronic Kidney Disease Population: A Clinical Research Protocol by Kevin Yau, Omosomi Enilama, Adeera Levin, Marc G. Romney, Joel Singer, Peter Blake, Jeffrey Perl, Jerome A. Leis, Robert Kozak, Hubert Tsui, Shelly Bolotin, Vanessa Tran, Christopher T. Chan, Paul Tam, Miten Dhruve, Christopher Kandel, Jose Estrada-Codecido, Tyler Brown, Aswani Siwakoti, Kento T. Abe, Queenie Hu, Karen Colwill, Anne-Claude Gingras, Matthew J. Oliver and Michelle A. Hladunewich in Canadian Journal of Kidney Health and Disease</p
Identification of Clk/Sty as a serine/arginine-rich protein kinase
No full textgrantor:
University of TorontoI have investigated the function of the protein kinase Clk/Sty. To discover proteins that may interact with Clk/Sty, I performed a two-hybrid screen in yeast using Clk/Sty as bait. Twenty-six mouse cDNA clones were identified in the screen. Four of these clones encode known transcription factors c-Myc, ZFP38, N-CoR, as well as a novel zinc finger protein. Other clones identified in the screen suggest a role for Clk/Sty in RNA processing. For instance, one of the clones, CARS-Cyp, encodes a novel arginine/serine-rich (RS) cyclophilin which co-localizes with splicing factors to nuclear speckles. Furthermore, six of the two-hybrid clones encode RNA-binding proteins. One of these clones is a mammalian homologue of the Drosophila splicing regulator tra-2. Three of the remaining five RNA-binding proteins are members of the SR family of essential splicing factors. I focused my studies on one member of the SR family, ASF/SF2, and found that Clk/Sty phosphorylated ASF/SF2 in vitro on sites that are also phosphorylated in vivo. As well, a catalytically inactive Clk/Sty co-localized to nuclear speckles with SR family members. Interestingly, overexpression of wild-type Clk/Sty led to disassembly of nuclear speckles resulting in redistribution of SR proteins. Therefore, Clk/Sty regulates the intranuclear distribution of SR proteins, presumably by phosphorylation. SRPK1 was the first kinase to be cloned and identified as an SR protein kinase. I found that SRPK1 phosphorylated RSRS repeats in ASF/SF2. Clk/Sty, on the other hand, phosphorylated SPR and KSKS repeats as well as RSRS repeats. Therefore, Clk/Sty displays a broader substrate specificity than SRPK1 in vitro, suggesting that Clk/Sty may phosphorylate more substrates than SRPK1 does in vivo. Pre-mRNA splicing is a complex process that is executed with high fidelity. In order to choose splice sites appropriately, the splicing machinery must be responsive to its external environment. I propose that Clk/Sty functions to transduce signals to this machinery enabling proper splice site selection.Ph.D
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