86,882 research outputs found
F-actin 결합 Cotl1 단백질의 미토콘드리아 다이나믹스 조절에 관여하는 역할
No full text비공개I. INTRODUCTION 1
II. MATERIALS AND METHODS 10
A. Annealing control primer (ACP)-based screening of differentially expressed genes 10
B. Plasmids, shRNA constructs and oligonucleotide sequence for siRNA 11
C. Cell culture and drugs treatment 12
D. Antibodies 12
E. Immunofluorescence 13
F. Confocal microscopy 13
G. Transmission electron microscopy 14
H. Primary cell culture 15
I. Quantification of mitochondrial length 16
J. Western blotting 16
K. Cell fractionation 17
L. Statistical analysis 17
III. RESULTS 18
A. Identification of differentially expressed genes among three types of HeLa cells with different mitochondrial morphology 18
B. Cellular localization of endogenous Cotl1 and overexpressed GFP-Cotl1 in HeLa, C2C12, and IMR90 cells 25
C. Changes in Cotl1 distribution in elongated mitochondria in shDrp1 cells 29
D. Changes in mitochondrial and F-actin morphologies in Cotl1-knock-down cells 32
E. Alterations in the expression of mitochondrial-shaping proteins in Cotl1-knockdown cells 37
F. Alterations in mitochondrial morphology and mitochondrial-shaping protein expression in primary cultured heart cells derived from Cotl1-knockout mouse 39
IV. DISCUSSION 42
V. CONCLUSION 46
REFERENCES 47
국문요약 55Maste
A statistical approach for comparing the performances of corner detectors
No full textCorner detectors are widely used in computer vision. This paper assesses several state-of-the-art corner detectors in terms of overall performance and the internal angles of corners using simple geometric shapes. This assessment is carried out using a statistically-valid null hypothesis approach, not previously used in computer vision. It is found that there are statistically significant differences in performance. Moreover, the null hypothesis approach is easy to use in comparing vision techniques
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Bulk viscosity in F(T, TG) gravity
No full textThe present paper is devoted to exploring the effect of bulk viscosity in the context of F(T, TG) gravity. We consider a time-dependent viscosity model with a particular expression of Hubble parameter. We evaluate viscous effective equation of state parameter for three well-known F(T, TG) models. The behavior of the accelerated expanding universe is explored graphically through the viscous equation of state parameter. This parameter indicates the phantom-dominated era as well as crosses the phantom divide line for all three models. We conclude that the universe shows a transition from quintessence to phantom region in the presence of bulk viscosity.The presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
[Newspaper Clipping: Author Claims Evidence of Second JFK Assassin #1]
No full textNewspaper article titled "Author Claims Evidence of Second JFK Assassin." The article states that author Richard J. Whalen concluded "that there is circumstantial evidence to support the theory of a second assassin in the shooting of President John F. Kennedy.
Also By The Same Author: AKTiveAuthor, a Citation Graph Approach to Name Disambiguation
No full textThe desire for definitive data and the semantic web drive for inference over heterogeneous data sources requires co-reference resolution to be performed on those data. In particular, name disambiguation is required to allow accurate publication lists, citation counts and impact measures to be determined. This paper describes a graph-based approach to author disambiguation on large-scale citation networks. Using self-citation, co-authorship and document source analyses, AKTiveAuthor clusters papers, achieving precision of 0.997 and recall of 0.818 over a test group of eight surname clusters
John F. Kennedy telegram to Roosevelt
No full textJersey Homesteads (later the Borough of Roosevelt) was established in the 1930s as an agro-industrial cooperative community. It was established specifically for urban Jewish garment workers, many of whom had emigrated from Europe. President John F. Kennedy sent a telegram to the citizens of Roosevelt, New Jersey, apologizing for not being able to attend the memorial dedication in honor of former President Franklin Delano Roosevelt. (Jersey Homesteads became Roosevelt in 1945 in honor of the president.) President Kennedy expressed his gratitude to the people of Roosevelt for constructing the memorial, and commented that it will serve as a constant reminder of Roosevelt's good works
Measuring the Coverage of Interest Point Detectors
No full textRepeatability is widely used as an indicator of the performance of an image feature detector but, although useful, it does not convey all the information that is required to describe performance. This paper explores the spatial distribution of interest points as an alternative indicator of performance, presenting a metric that is shown to concur with visual assessments. This metric is then extended to provide a measure of complementarity for pairs of detectors. Several state-of-the-art detectors are assessed, both individually and in combination. It is found that Scale Invariant Feature Operator (SFOP) is dominant, both when used alone and in combination with other detectors
Logarithmic variance profiles and the corresponding f-1 spectra of temperature fluctuations in turbulent Rayleigh-Bénard convection
No full textWe report experimental results for the temperature variance 2(z) and the corresponding frequency spectra P(f) in turbulent Rayleigh-Bénard convection (RBC) in a cylindrical sample of aspect ratioT= D/L = 1:00 (D = 1:12 m is the diameter and L = 1:12 m the height). The measurements were conducted in the Rayleigh-number range 1011 < Ra < 1:35 1014 and Pr ' 0:8. For Ra = 1:35x1014, 2(z) could be described well by a logarithmic dependence on the vertical position z in a range of z 1 < z < z 2 with z 1 ' 70 and z 2 = 0:1L. Here L=(2Nu) is the thickness of a thin thermal sublayer adjacent to the horizontal plate where the heat flux (denoted by the Nusselt number Nu) is carried mostly by thermal diffusion. In the log layer, we found that the temperature spectra had a significant frequency range over which P(f) f with close to 1. As Ra decreased, increased so that the log layer became thinner. At Ra = 2:05 1011, z 2 < z 1 and therefore there was no range for a log layer. Correspondingly, the temperature spectrum near the horizontal plate did not have the f1 scaling form either
Maine author Franklin F. Gould recalls his first glimpse of the outside world
No full textMaine author Franklin F. Gould recalls his first glimpse of the outside world as he relates how, as a young farm boy in the late 1800\u27s, he drove his father\u27s horses on an errand to an icebound river
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