19 research outputs found
The Role of Oxidative Stress in adverse uterine environment: The new explanation on developmental origin of adult diseases
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Classic Antiphospholipid and Antiphosphatidylserine Antibody Profile in Suspected Antiphospholipid Antibody Syndrome Patient
Get PDFObjective: To compare the classic examination results of antiphospholipid
(aPS) and antiphospatidylserine (aPL) antibody profile to
establish the diagnosis from suspected antiphospholipid antibody
syndrome (APS) patient in order to state the subsequent treatment
strategies.
Method: This descriptive cross-sectional study design was conducted
at outpatient clinics of Dr. Cipto Mangunkusumo Hospital
(RSCM) from January to December 2015. The laboratory test was
held in Clinical Pathology Laboratory RSCM/Faculty of Medicine
Universitas Indonesia (FKUI) and in corporation with Prodia laboratory.
Result: All of normal patients did not have positive result in any
laboratory examination (Lupus Anti-coagulant (LA), anticardiolipin
(aCL), anti-?2 glycoprotein I (anti-?2GPI), and aPS). In patient suspected
APS, 11 (37.1%) patients had positive aCL, 7 (25.9%) patients
had positive anti-?2GPI, and 11 (37.1%) patients had positive
aPS. The most positive cross laboratory examination was between
aCL and aPS (25.9%). In this study, we found the most positive test
result was aCL and aPS (62.9%). From this study, suspected APS patient
who had negative result in classic laboratory examination, but
showing the positive result in aPS was in 5 (18.5%) patients.
Conclusion: All normal pregnant patients do not have any positive
classic examination and aPS. Meanwhile, in patients with suspected
APS, less than 20% patients show positive result of aPS with negative
result in classic laboratory examination.
Keywords: antiphospatidylserine, antiphospolipid syndrom
Profile of Estrogen Metabolism in Endometriosis Patients
Get PDFObjective: To assess the estrone (E1), estradiol (E2) and estriol (E3) blood level and its ratio (E2:E1, E2:E3 and E1:E3) between women with and without endometriosis.
Method: We performed an analytical cross sectional study with 27 women with endometriosis and 27 women without endometriosis who met the inclusion criteria. The samples were recruited in Dr. Cipto Mangunkusumo hospital and other satellite hospitals from October 2012 to April 2013. The blood level of estrogen metabolites was examined by enzyme-linked immunosorbent assay (ELISA). Comparison between the two groups was analyzed by using Mann- Whitney test.
Result: The level of estrone was found to be lower in endometriosis group compared to that in the control group (54.66 pg/ml vs 73.52 pg/ml, p=0.229). Similarly, the levels of estradiol and estriol were lower in endometriosis group (29 pg/ml vs 35 pg/ml, p=0.815 and 1.11 pg/ml vs 1.67 pg/ml, p=0.095, consecutively). The E2:E1 ratio was higher in endometriosis group (0.51 pg/ml vs 0.38 pg/ml, p=0.164), as well as E2:E3 ratio (26.53 pg/ml vs 21.11 pg/ml, p=0.223) and the E1:E3 ratio (58.55 pg/ml vs 50.28 pg/ml, p=0.684). However, all those differences were not statistically significant.
Conclusion: The estrone, estradiol and estriol levels in women with endometriosis were lower compared to those in women without endometriosis. The ratio of E2:E1, E2:E3 and E1:E3 were higher in endometriosis group. However, all those differences failed to reach statistical significance.
Keywords: endometriosis, estradiol, estriol, estrogen, estron
Chronic Pelvic Pain and Associated Clinical Characteristics among Women in a Tertiary Care Center in Indonesia
Get PDFObjective: To determine the prevalence and various aspects associated with female chronic pelvic pain.
Methods: During the period of January to March 2016, a crosssectional study was conducted at the Women’s Health Clinic of Dr.
Cipto Mangunkusumo Hospital, Jakarta, Indonesia. Subjects were female who complained of pelvic pain for more than 6 months. We performed history taking, physical examination, and laboratory investigations including hs-CRP serum examination. We calculate the prevalence and describe the clinical characteristics and diagnosis of the patient. Quality of life and levels of hs-CRP were compared between the group of mild and severe pain.
Results: In this study, the prevalence of CPP was 9.78%. We found 96.9% of gynecological disorders, 1% of urological disorders, and 2.1% of musculo-skeletal disorders. The most common diagnosis is endometriosis. The Clinical characteristics of patients were found 62.9% suffer for 6 months - 1 year with the intensity of pain (VAS) 7-10 as much as 51.5%. Levels of hs-CRP serum was around 1.99 (0.00 - 404, 53). We found a decreased in the quality of life of the patient. The physical domain score was 56 (38-81); the psychology domain score was 56 (31-100); the domain of social relationships was 59 (25-75); and the environmental domain score was 56 (31-94).
Conclusion: Endometriosis is the most common diagnosis in female chronic pelvic pain of reproductive age. The pain causes a
decreased in quality of life who suffer from it.
[Indones J Obstet Gynecol 2017; 5-3: 154-159]
Keywords: chronic pelvic pain, hs- CRP, intensity of pain, quality of lif
Profile of Estrogen Metabolism in Endometriosis Patients: Profil Metabolisme Estrogen pada Penderita Endometriosis
Get PDFObjective: To assess the estrone (E1), estradiol (E2) and estriol (E3) blood level and its ratio (E2:E1, E2:E3 and E1:E3) between women with and without endometriosis.
Method: We performed an analytical cross sectional study with 27 women with endometriosis and 27 women without endometriosis who met the inclusion criteria. The samples were recruited in Dr. Cipto Mangunkusumo hospital and other satellite hospitals from October 2012 to April 2013. The blood level of estrogen metabolites was examined by enzyme-linked immunosorbent assay (ELISA). Comparison between the two groups was analyzed by using Mann- Whitney test.
Result: The level of estrone was found to be lower in endometriosis group compared to that in the control group (54.66 pg/ml vs 73.52 pg/ml, p=0.229). Similarly, the levels of estradiol and estriol were lower in endometriosis group (29 pg/ml vs 35 pg/ml, p=0.815 and 1.11 pg/ml vs 1.67 pg/ml, p=0.095, consecutively). The E2:E1 ratio was higher in endometriosis group (0.51 pg/ml vs 0.38 pg/ml, p=0.164), as well as E2:E3 ratio (26.53 pg/ml vs 21.11 pg/ml, p=0.223) and the E1:E3 ratio (58.55 pg/ml vs 50.28 pg/ml, p=0.684). However, all those differences were not statistically significant.
Conclusion: The estrone, estradiol and estriol levels in women with endometriosis were lower compared to those in women without endometriosis. The ratio of E2:E1, E2:E3 and E1:E3 were higher in endometriosis group. However, all those differences failed to reach statistical significance.
Keywords: endometriosis, estradiol, estriol, estrogen, estron
Postoperative Pain Survival and Correlating Factors in Endometriosis Patients: Kesintasan Keluhan Nyeri Pasca Pembedahan pada Pasien Endometriosis serta Faktor-Faktor yang Mempengaruhi
Get PDFAbstract
Objective: To determine the survival of pain complaints at 3, 6, 9 and 12 months and the correlation between age at diagnosis, age of menarche, parity, stage of disease and post-operative medication in endometriosis patient at RSUPN Dr. Cipto Mangunkusumo.
Methods: This was a prospective cohort study with survival analysis method of 139 women of productive age with endometriosis who came to Gynecology Outpatient Clinic in Dr. Cipto Mangunkusumo Hospital from January 2015 to January 2017. The patients were observed at 3 months, 6 months, 9 months and 12 months after the surgery.
Results: Survival of pain complaints at 3, 6, 9 and 12 months after endometriosis operation was 99.2%, 96.6%, 93% and 88.4% respectively. There was no significant correlation between post-operative pain survival and age of diagnosis (p=0.138), age of menarche (p=0.492), parity (p=0.110) and stage of disease (p=0.908). There was a significant correlation between post-operative medical therapy and pain complaints survival. Subjects who were given medication had a risk of 0.26 times (CI 95% 0.081-0.857) pain recurrence compared to those who were not.
Conclusion: Pain complaints survival were significantly associated with post-operative medical therapy, but not associated with age, age of menarche, parity and stage of disease.
Keywords: endometriosis, postoperative medical therapy, postoperative pain, survival
Abstrak
Tujuan: Mengetahui kesintasan keluhan nyeri pada 3, 6, 9 dan 12 bulan dan hubungan antara usia saat diagnosis, usia menarke, paritas, stadium penyakit dan terapi medisinalis pasca pembedahan pada pasien endometriosis di RSUPN Dr. Cipto Mangunkusumo.
Metode: Penelitian kohort prospektif pada 139 wanita usia produktif dengan metode analisis kesintasan pada pasien endometriosis yang datang ke Poliklinik Ginekologi RSUPN Cipto Mangunkusumo pada bulan Januari 2015 hingga Januari 2017. Pasien kemudian diobservasi pada bulan ke 3, 6, 9 dan 12 setelah operasi.
Hasil : Kesintasan keluhan nyeri pada bulan ke 3, 6, 9 dan 12 berturut-turut adalah 99,2%, 96,6%, 93% dan 88,4%. Tidak ada hubungan yang bermakna antara kesintasan nyeri pasca pembedahan dengan usia saat diagnosis (p=0,138), usia saat menarke (p=0,492), paritas (p=0,110) dan stadium penyakit (p=0,908). Terdapat hubungan yang bermakna antara terapi medisinalis pasca pembedahan dan kesintasan keluhan nyeri. Subyek yang mendapatkan terapi medisinalis pasca pembedahan memiliki risiko 0,26 kali mengalami kekambuhan nyeri dibandingkan dengan yang tidak.
Kesimpulan : Kesintasan keluhan nyeri memilki hubungan yang bermakna dengan terapi medisinalis pasca pembedahan namun tidak memiliki hubungan dengan usia saat diagnosis, usia menarke, paritas dan stadium penyakit.
Kata kunci: endometriosis, kesintasan, nyeri pasca pembedahan, terapi medisinalis pasca pembedaha
Role of Glycated Albumin during Pregnancy
Get PDFObjective: To determine the glycated albumin profile during
pregnancy with normal glycemic status.
Methods: We recruited 60 pregnant women between 21 and 36
weeks of gestation. We conducted several laboratory tests, such as
glycated albumin, blood glucose, and albumin. These parameters
were compared among four groups of gestational age (21-24 weeks,
25-28 weeks, 29-32 weeks, and 33-36 weeks) using ANOVA or
Kruskal-Wallis test continued by Post-hoc test.
Results: Glycated albumin was not statistically different among the
groups. Albumin level of 33-36 weeks of gestation women (3.6 (SD
0.2) g/dl) was lower than 21-24 weeks of gestation women (3.8 (SD
0.2) g/dl).
Conclusion: Glycated albumin level is not affected by gestational
age. Therefore, glycated albumin may be used as glycemic status
indicator during pregnancy from 21 to 36 weeks.
[Indones J Obstet Gynecol 2017; 5-1: 16-18]
Keywords: HbA1c, glycated albumin, glycemic status, pregnanc
PPARy Expression in Eutopic and Ectopic Endometrium of Reproductive Age Women with Endometriosis
Get PDFObjective: To evaluate the expression of PPARy receptor and to
compare its expression in eutopic and ectopic endometrium in
women with endometriosis.
Method: This is a cross sectional study. Ten female subjects with endometriosis
that underwent laparoscopy or laparotomy who fulfilled
the inclusion criteria were recruited by consecutive sampling.
Two samples were taken, eutopic endometrium and ectopic endometrium
from endometriosis cyst wall during surgery of each
subject. PPARy expression was examined by two-step RT-qPCR. Our
data was statistically examined using the paired t-test and Pearson’s
correlation test.
Result: PPARy was found to be expressed in eutopic and ectopic endometrium
of women with endometriosis using the RT-qPCR method.
The expression of PPARy was not statistically different in
eutopic and ectopic endometrium (1.16 relative fold vs 1.25 relative
fold; p=0.26). By Pearson’s correlation there was a weak positive
correlation between PPARy expression of eutopic and ectopic endometrium
(r=0.16).
Conclusion: PPARy was detected by two-step RT-qPCR in eutopic
and ectopic endometrium of women with endometriosis. Semiquantification
of PPARy expression showed that there was no significant
difference between PPARy expression in eutopic and ectopic endometrium
of women with endometriosis. There was a weak positive
correlation between PPARy expression in eutopic and ectopic endometrium
of women with endometriosis.
[Indones J Obstet Gynecol 2015; 3-4: 200-205]
Keywords: endometriosis, PPARy, two-step RT-qPC
The mIRNA519a-3p and NKG2D in endometriosis.
No full textBackground: Natural killer (NK) cells play a role in pathogenesis of endometriosis. Lower expressions of NK cells receptor group 2D (NKG2D) ligands inhibits cytotoxic activity of NK cells; a common immunity avoidance mechanism in neoplasms. Literatures have proven miRNAs regulatory effect on NKG2D expression. There has been no specific biomarker for diagnosing endometriosis. Non-invasive means of diagnosing endometriosis may reduce well-known risks of invasive method of diagnosis and yield better results.
Purpose: To investigate the correlation between miRNA-519a-3p expression with NKG2D ligands (MICA, MICB, ULBP 1-6) on endometriosis and non-endometriosis patients.
Methods: This was a cross-sectional study held in five centers: dr. Cipto Mangunkusumo General Hospital, Pelni Hospital, Bunda Hospital, YPK Mandiri Hospital, and Primaya Evasari Hospital from October 2020 to July 2021. miRNA and NKG2DL analysis were done in Human Reproduction, Infertility and Family Planning (HRIFP) cluster at IMERI FKUI.
Results: We obtained 19 patients in each study groups. NKG2D ligands and miRNA519a-3p relative expressions were not significantly different (p > 0.05). Increased miRNA519a-3p expression negatively affected NKG2D ligands expression. A decrease in ULBP1 and an increase in ULBP2 increased the probability for endometriosis. NKG2D ligands expression may be influenced by infection, pro-inflammatory cytokine production, dan polymorphism. NKG2D ligands expression level can be different depending on the origin of the sample. Lower expression of miRNA519a-3p indirectly inhibits tumor apoptosis by lowering NKG2D ligands, caspase, or mRNA.
Conclussion: We did not manage to establish a correlation between NKG2D ligands with miRNA519a-3p in endometriosis and non-endometriosis patients
Increase of PlGF (Placental Growth Factor) Level After Administration of Dydrogesterone in Pregnancy
Get PDFAim: To observe the effect of Dydrogesterone administration in pregnancy on PlGF levelMethods: This is a randomized controlled clinical trial. Study population has been divided into two groups. Group A consists of 20 women who receive only Folic acid 5 mg a day for 4 weeks time. Group B consists of 20 women who receive Dydrogesterone 2x10 mg a day and Folic acid 5 mg a day for 4 weeks. PlGF has been measured twice. First measurement was done before drug administration, while the second measurement has been done during 18th weeks of pregnancy. The changes on PlGF level before and after treatment from each group has been analyzed using SPSS 17.Results: 40 pregnant women have been recruited for this study. There are no differences based on the patient’s age, number of pregnancy and parity, gestational age and body weight between each group. The mean levels of PlGF in both groups before intervention shows no significant difference (p = 0091 or p> 0.05), 40.80 pg/mL vs. 25.95 pg / mL. The mean levels of PlGF in group A after 4 weeks administration of Folic acid is 89.60 pg / mL. It shows the escalation of 48.8 pg / mL. The elevation of PlGF level in group A shows significant difference (p = 0.000 or p <0.05) after 4 weeks Folic acid treatment.The mean levels of PlGF in group B after 4 weeks administration of Dydrogesterone and Folic acid is 212.15 pg / mL. It shows the escalation of 186.20 pg / mL. The elevation of PlGF level in group B shows significant difference (p = 0.000 or p <0.05) after 4 weeks Dydrogesterone and Folic acid treatment.Conclusion: Dydrogesterone treatment can increase the level of PlGF
