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    Trichoderma dumbbelliforme sp. nov. an undescribed fungus of order Hypocreales from India

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    Kamil, Deeba, Toppo, R. Sudeep, Choudhary, Shiv Pratap (2021): Trichoderma dumbbelliforme sp. nov. an undescribed fungus of order Hypocreales from India. Phytotaxa 520 (3): 285-295, DOI: 10.11646/phytotaxa.520.3.8, URL: http://dx.doi.org/10.11646/phytotaxa.520.3.

    FIGURE 1 in Trichoderma dumbbelliforme sp. nov. an undescribed fungus of order Hypocreales from India

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    FIGURE 1. Phylogenetic tree based on maximum parsimony analysis with 1000 replications of the tef-1dataset.Published as part of Kamil, Deeba, Toppo, R. Sudeep & Choudhary, Shiv Pratap, 2021, Trichoderma dumbbelliforme sp. nov. an undescribed fungus of order Hypocreales from India, pp. 285-295 in Phytotaxa 520 (3) on page 289, DOI: 10.11646/phytotaxa.520.3.8, http://zenodo.org/record/553042

    FIGURE 9 in Polyphasic taxonomy of Indian Trichoderma species

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    FIGURE 9. Trichoderma erinaceum (A) Growth on PDA, (B) Pustules, (C) Reverse of the plate, (D) Conidiophore branching, (E,F) Phialide disposition, (G) Spores, (H) Chlamydospores.Published as part of Thokala, Prameeladevi, Narayanasamy, Prabhakaran, Kamil, Deeba & Choudhary, Shiv Pratap, 2021, Polyphasic taxonomy of Indian Trichoderma species, pp. 1-27 in Phytotaxa 502 (1) on page 12, DOI: 10.11646/phytotaxa.502.1.1, http://zenodo.org/record/542489

    Trichoderma saturnisporum Hammill, Mycologia

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    7. Trichoderma saturnisporum Hammill, Mycologia 6:, 970. Figure 9 Section—Longibrachiatum Description: Colony: 7.0– 8.5 cm growth was observed in 4 days on PDA medium. White fluffy pustules turning to grey-green colour with the age. The pustules uniformly spreaded throughout the plate. Reverse of the plate is yellow-green. Conidiophores: Conidiophores arising from the aerial mycelium of developed pustules, asymmetrically branched, the branches producing phialides directly or rebranching, the secondary branches producing phialides along the length and ending in a single phialide; sometimes the main axis of a conidiophore is terminating in a sterile, septate, branched or unbranched, hypha-like elongation. Phialides: Phialides mainly arising singly, less frequently in appressed to divergent whorls of 2–3, typically curved, ampulliform to broadly lageniform, sometimes hooked or sinuous. Conidia: Conidia green, smooth walled but with conspicuous sinuate, bullate or wing like inflations of the outer wall, ellipsoidal, 4.5–7.5 × 3.5–4.5 µm. Chlamydospores: Chlamydospores present, globose, 6.0–12.0 µm dia. Culture examined:ITCC 7273 (Soil, Chandigarh) ITCC 7274 (Soil, Palampur, HP). Diagnostic features: Conidiophores are asymmetrically branched producing single curved, ampulliform, lageniform, hooked or sinuous phialides directly.Conidia have conspicuous wing like inflations on the outer wall. Ecology and habitats: Wood, herbaceous tissue, soil.Published as part of Thokala, Prameeladevi, Narayanasamy, Prabhakaran, Kamil, Deeba & Choudhary, Shiv Pratap, 2021, Polyphasic taxonomy of Indian Trichoderma species, pp. 1-27 in Phytotaxa 502 (1) on page 21, DOI: 10.11646/phytotaxa.502.1.1, http://zenodo.org/record/542489

    Trichoderma crassum Bissett, Can. J. Bot.

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    6. Trichoderma crassum Bissett, Can. J. Bot. 69: 76– 79, 99. Figure 8 Section— Pachybasium Description: Colony: 4.0–5.0 cm growth was observed in 4 days on PDA medium. Slow growing, white floccose mycelium becoming bright yellow then turns to grey-green and then grey. Concentric rings of white, grey-green, grey and yellow are very conspicuous. Cushion like flat pustules spread throughout the plate. The reverse of the plate was yellowishorange. Conidiophores: Conidiophores in minute pustules or effuse, branching irregularly, the apical part of the main conidiophores axis is straight or flexuous, sterile and unbranched. Long unbranched conidiophores with one or two phialides at the tip are sometimes present. Phialides: Phialides mainly arising in crowded whorls of 2–5 on terminal and lateral branches, ampulliform to lagniform and subulate. Conidia: Conidia broadly ellipsoidal to obovoid with the base slightly narrower, 3.0–5.0 × 3.0–3.5 µm, smooth and green. Chlamydospores: Chlamydospores are globose, 4.0–6.0 µm diam. Culture examined: ITCC 7293 (Soil, Assam). Diagnostic features: White, grey-green, grey and yellow conspicuous concentric rings with a cushion like pustules and yellowish-orange at the reverse of the plate. Conidiophores apical part straight or flexuous, sterile and unbranched with crowded Phialides. Ecology and habitats: Wood and soil.Published as part of Thokala, Prameeladevi, Narayanasamy, Prabhakaran, Kamil, Deeba & Choudhary, Shiv Pratap, 2021, Polyphasic taxonomy of Indian Trichoderma species, pp. 1-27 in Phytotaxa 502 (1) on page 11, DOI: 10.11646/phytotaxa.502.1.1, http://zenodo.org/record/542489

    Trichoderma erinaceum Bissett, Kubicek & Szackacs, Can. J. Bot.

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    7. Trichoderma erinaceum Bissett, Kubicek & Szackacs, Can. J. Bot. 8: 8, 00. Figure 9 Section— Trichoderma Description: Colony: 7.0– 8.5 cm growth was observed in 4 days on PDA medium. White mycelium producing yellow pustules turning to dark green color with the age. The pustules uniformly spread throughout the plate leaving a concentric green ring at the center. The reverse of the plate is colorless. Conidiophores: The main axis terminates in a septate elongation with a single phialide at its tip and fertile branches arising near the base. Conidiophore branches arising at angles of 90° or less concerning the main axis, paired or not, rebranching sparingly to produce phialides directly or at the tips of short secondary branches. Phialides: Phialides arising from branches are solitary or in whorls of 2 or 3, straight, cylindrical, flask-shaped and swollen in the middle. Conidia: Ellipsoidal to broadly ellipsoidal, 4.00–5.50 × 3.5–4.5 µm, green. Chlamydospores: Chlamydospores terminal to intercalary, globose to subglobose, 8.00–13.00 µm. Cultures examined: ITCC 7287 (Withania somnifera rhizosphere, Delhi); ITCC 7288 (Groundnut rhizosphere, Tirupati, AP); ITCC 7289 (Soil, SUKAST, Jammu, J&K). Diagnostic features: Conidiophores arising at 90° and ends with a single phialide with the fertile branches at the base and produce solitary and flask-shaped phialides directly on secondary branches. Ecology and habitats: Soil, endophyte of woody tissue of Theobroma cacao in Peru.Published as part of Thokala, Prameeladevi, Narayanasamy, Prabhakaran, Kamil, Deeba & Choudhary, Shiv Pratap, 2021, Polyphasic taxonomy of Indian Trichoderma species, pp. 1-27 in Phytotaxa 502 (1) on page 11, DOI: 10.11646/phytotaxa.502.1.1, http://zenodo.org/record/542489

    Trichoderma hamatum Bain., Bull. Soc. Mycol.

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    9. Trichoderma hamatum (Bon.) Bain., Bull. Soc. Mycol. France:, 906. Figure Section— Pachybasium Description: Colony:7.0– 8.5 cm growth was observed in 4 days on PDA medium. Mycelium is mostly submerged, less aerial mycelium which is floccose and white. Compact cushioned pustules looking velvety due to the presence of numerous, flexuous sterile conidiophore apices. These pustules are white at first, becoming yellow, then pea-green followed by olive green. Colorless at the reverse of the Petri plate. Conidiophores: Conidiophores in pustules are broad, comprising a regular, undulate and humate sterile elongation with the phialides arising near the base. The lateral branches typically comprise one or a few, broad cells with phialides arising at the tip and along the length. The internodes between branches are short and the phialides densely clustered. Phialides: Phialides are short, broadly ellipsoidal to ovoidal, pyriform and ampulliform, formed on small branches, smooth - walled, hyaline or pale green color. Conidia: Oblong to ellipsoidal and smooth, 5.0–6.0 × 3.0–4.0 µm, green. Chlamydospores: Chlamydospores terminal and intercalary, globose, 4.0–8.0 µm diam. Cultures examined: ITCC 7271 (Rice soil, Cuttack, Odisha); ITCC 7272 (Soil, Kochi, Kerala); ITCC 7270 (Soil, Dehradun, Uttarakhand). Diagnostic features: Compact cushioned pustules white at first, becoming yellow, then green in colour. Conidiophores with non-fertile, stout, hamate and undulate elongations, with the short, ovoidal or pyriform densely clustered phialides near the base. Ecology and habitats: Soil.Published as part of Thokala, Prameeladevi, Narayanasamy, Prabhakaran, Kamil, Deeba & Choudhary, Shiv Pratap, 2021, Polyphasic taxonomy of Indian Trichoderma species, pp. 1-27 in Phytotaxa 502 (1) on page 14, DOI: 10.11646/phytotaxa.502.1.1, http://zenodo.org/record/542489

    Trichoderma ghanense Doi, Abe & J. Sugiyama, Bull. Natl. Sci. Mus. Ser. B (Bot.

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    8. Trichoderma ghanense Doi, Abe & J. Sugiyama, Bull. Natl. Sci. Mus. Ser. B (Bot.):, 987. Figure 0 Section—Longibrachiatum Description: Colony: 7 – 8.5 cm in four days. Aerial hyphae cottony grown throughout the plate. Artemisia green white cushionlike small pustules formed throughout the plate but concentrating more than 2 cm away from the edge of the plate. The white pustules turned to green after conidiation. Reverse of the plate was colorless. Conidiophores: Conidiophores typically consisting of a strongly developed central axis from which arise, toward the tip, solitary phialides and further from the tip progressively longer, often paired, secondary branches. Phialides arising directly from secondary branches, typically not in whorls. Phialides: Phialides cylindrical or straight or hooked. Conidia: Conidia green, ellipsoidal, 6.0–7.5× 4.0–4.5 µm and smooth. FIGURE 0. Trichoderma ghanense ( A ) Growth on PDA, ( B ) Pustules with ooze, ( C ) Reverse of the plate, ( D ) Conidiophore branching, ( E,F,G ) Phialide disposition, ( H ) Spores, ( I ) Chlamydospores. Chlamydospores: Chlamydospores abundant, globose, 7.0–12.0 µm in dia. and smooth. Culture Examined: ITCC 7279 (Soil, Arunachal Pradesh). Diagnostic features: Greenish white cushion-like small pustules, Conidiophore bearing cylindrical or straight or looked solitary phialides. Chlamydospores are abundant and globose. Ecology and habitats: Soil. FIGURE. Trichoderma harzianum ( A ) Growth on PDA, ( B ) Pustules, ( C ) Reverse of the plate; ( D, E ) Conidiophore branching, ( F,G ) ampulliform phialides, ( H ) Spores, ( I ) Spores under SEM, ( J ) Chlamydospores. FIGURE. Trichoderma hamatum ( A ) Growth on PDA, ( B ) Pustules, ( C ) Reverse of the plate, ( D ) pustule, ( E.F ) Conidiophore branching, ( G,H,I ) Phialide disposition, ( J ) Spores, ( K ) Chlamydospores.Published as part of Thokala, Prameeladevi, Narayanasamy, Prabhakaran, Kamil, Deeba & Choudhary, Shiv Pratap, 2021, Polyphasic taxonomy of Indian Trichoderma species, pp. 1-27 in Phytotaxa 502 (1) on pages 11-13, DOI: 10.11646/phytotaxa.502.1.1, http://zenodo.org/record/542489

    Trichoderma virens Arx

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    0. <i>Trichoderma virens</i> (Miller, Giddens & Foster) Arx, Nova Hedwigia Beih. 87: 88, 987. Figure <p>Section— Pachybasium</p> <p>Description:</p> <p>Colony: 6.0–7.0 cm growth was observed in 4 days on PDA medium. Conidiation effuse covering the entire surface of the plate, or forming spreading, light yellowish green flat pustules concentrated near the margin, later turning dark green. Reverse colourless and sometimes drab coloured.</p> <p>Conidiophores: Conidiophores arising in clusters in lateral branches from undifferentiated aerial mycelium, at the base sterile and un-branched, but the upper part fertile toward the apex,each branch terminating in a penicillus of (2–)3–6 closely appressed phialides. The entire branching system irregular and uncrowded.</p> <p>Phialides: Phialides mainly arising in closely appressed whorls of 2–5 on terminal branches, less frequently in pairs or singly, straight, lageniform to ampulliform and sometimes laginiform to subulate, base constricted, swollen in the middle, attenuate at the tip.</p> <p>Conidia: Broadly ellipsoidal to obvoidal and minutely warted at high magnifications (SEM), 3.5–8.0 × 3.0–5.0 µm, dark green. Conidia from adjacent phialides often coalescing into large gloeoid masses.</p> <p>Chlamydospores: Chlamydospores abundant, terminal and intercalary, globose to sub-globose, 6.0–12 µm.</p> <p>Cultures examined: ITCC6461 (Soil, New Delhi); ITCC 6470 (Soil, New Delhi).</p> <p>Diagnostic features: In the plate light yellowish green flat pustules concentrated near the margin turning to dark green.Conidiophore branching terminating in a penicillus of (2–)3–6 closely appressed phialideswhich are straight, lageniform to ampulliform, sometimes subulate, base constricted, swollen in the middle, attenuate at the tip. Conidia obvoidal and minutely warted. Conidia from adjacent phialides often coalescing into large gloeoid masses.</p> <p>Ecology and habitats: Wood, soil, other fungi.</p>Published as part of <i>Thokala, Prameeladevi, Narayanasamy, Prabhakaran, Kamil, Deeba & Choudhary, Shiv Pratap, 2021, Polyphasic taxonomy of Indian Trichoderma species, pp. 1-27 in Phytotaxa 502 (1)</i> on page 23, DOI: 10.11646/phytotaxa.502.1.1, <a href="http://zenodo.org/record/5424892">http://zenodo.org/record/5424892</a&gt
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