1,720,991 research outputs found
Species-specific identification of equine cyathostomes resistant to fenbendazole and susceptible to oxibendazole by macroarray probing
No full textCyathostome populations in horses on two farms located in central Italy with a history of fenbendazole (FBZ) resistance were investigated with the Faecal Egg Count Reduction Test to evaluate the susceptibility to oxibendazole and moxidectin. Faecal eggs were collected pre- and post-treatment on each farm and molecularly examined with a Reverse Line Blot (RLB) assay able to unequivocally detect and identify 13 cyathostome species. Resistance to FBZ was confirmed on both farms, while oxibendazole and moxidectin demonstrated 97% and 100% efficacy, respectively. Overall eight species of cyathostomes (Coronocyclus labiatus, Cylicocyclus ashworthi, Cylicocyclus nassatus, Cyathostomum catinatum, Cylicostephanus longibursatus, Cylicostephanus goldi, Cylicostephanus calicatus and Cylicocyclus insigne) were identified in pre-treatment samples. Coronocyclus labiatus and C. goldi were identified after treatment with FBZ while C. calicatus and C. labiatus were shown to be <100% susceptible to oxibendazole. These data confirm that resistance to benzimidazoles is established in cyathostome populations from horse farms in Italy and that they are susceptible to moxidectin. The oxibendazole has been successfully demonstrated for the first time as effective against Italian populations of cyathostomes resistant to other benzimidazoles. The RLB assay herein used showed to be useful to study the distribution of these parasitic populations at species level under field conditions and could represent a powerful tool in broader investigation of drug resistance in horse farms from several countries
Species-specific identification of equine cyathostomes resistant to fenbendazole and susceptible to oxibendazole and moxidectin by macroarray probing
No full textValutazione dell’efficacia dell’oxibendazolo e della moxidectina nei confronti dei cyathostomini resistenti al fenbendazolo
No full textHaplotypic variability within the mitochondrial gene encoding for the cytochrome c oxidase 1 (cox1) of Cylicocyclus nassatus (Nematoda, Strongylida): evidence for an affiliation between parasitic populations and domestic and wild equid hosts
No full textCyathostome third stage larvae capability and species-specific aptitude to develop in vitro
No full textNew method for simultaneous species-specific identification of equine strongyles (Nematoda, Strongylida) by Reverse Line Blot hybridization
No full textThe ability of a reverse line blot (RLB) assay to identify 13 common species of equine small strongyles (cyathostomins) and to discriminate them from three Strongylus spp. (large strongyles) was demonstrated. The assay relied on the specific hybridization of PCR-amplified intergenic spacer DNA fragments of the nuclear ribosomal DNA to membrane-bound species-specific probes. All cyathostomins examined were unequivocally identified and simultaneously discriminated from each other and from three large strongyles (Strongylus edentatus, Strongylus equinus, and Strongylus vulgaris). This assay will enable the accurate and rapid identification of equine cyathostomins irrespective of their life cycle stage, opening important avenues for a better understanding of their biology and epidemiology and of the pathogenesis of cyathostomin-associated disease. In particular, this RLB method promises to be a powerful diagnostic tool to determine the roles of individual species in the pathogenesis of mixed infections and to elucidate some aspects of cyathostominosis. Also, it could represent a basic step toward the development of a rapid and simple molecular test for the early detection of drug-resistant genotypes of horse strongyle species.[...
Occurrence of anthelmintic resistant equine cyathostome populations in Central and Southern Italy
No full textIn the present survey, 276 horses bred on 16 farms located in central and southern Italy were investigatedfor the presence of drug resistant cyathostomes by a Fecal Egg Count Reduction Test (FECRT). Sixteen to20 animals were selected on each farm and randomly assigned to one of four equally sized treatment groups.Groups were treated with fenbendazole, pyrantel pamoate, ivermectin or moxidectin. Resistance tofenbendazole was declared on six farms (37.5%) and suspected in two farms (12.5%), with FECR valuesranging from 41% to 88.3%. Resistance to pyrantel was found in two farms (12.5%) and was suspected inone case (6.2%), with FECR values ranging from 43% to 85.4%. Macrocyclic lactones remained effectiveon all farms. Only cyathostome third stage larvae (L3) were found in fecal cultures after treatment. Thispaper reports the first wide survey conducted in Italy for anthelmintic resistance in equine cyathostomes.The results indicate that multiple drug resistant equine cyathostomes are present in the central and southernregions of Italy. These data call for a geographically and numerically broader investigation of horse farms inall regions and for the development and implementation -among veterinarians, owners and managers of a plan to reduce the expansion of these anthelmintic resistant populations and control these importantparasites.[...
New insights into molecular identification of equine cyathostomes: significant scope for improved diagnosis and infection control
No full text- …
