1,721,635 research outputs found
Configuration control of liquid crystal in the droplet dispersed in the polymer with mesogenic side group
No full textA side chain liquid crystalline polymer was applied to control the alignment of liquid crystal molecules at the interface to the direction of mesogenic side group, The aspects of the alignment were monitored by observing the droplet in the PDLC film, When the composition of the mesogenic side group increases, the configuration of LC droplets changes from bipolar to radial one as designed
Electro-optic properties of polymer dispersed liquid crystal films under the controlled interface conditions
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Development of Serum-Free Medium for the Production of Humanized Antibody from Recombinant Chinese Hamster Ovary Cells
No full textModification of poly(vinyl cinnamate) photo-alignment layers using polymerizable mesogens
No full textPolymerizable mesogens were blended with poly(vinyl cinnamate) (PVCi) in order to improve the alignment properties of liquid crystals (LC) on the layer surface. Two types of cyanobiphenyl monomers, which have short (n=0) and long (n=11) spacers, were prepared, respectively. Polarized UV exposure at the elevated temperature increased the inclusion of the monomers into the network of the photo-products. Compositional studies showed that the short-spacer monomer improved homogeneous LC alignment and thermal stability, whereas the long-spacer monomer contributed to generate homeotropic LC alignment
Control of Electro-Optic Property Using Ferroelectric liquid crystal Mesogen at Polymer Interface In PDLC
No full textLiquid crystal alignment induced by suspension flow on polyimide surfaces
No full textIn order to overcome the drawbacks of the conventional rubbing method, wet rubbing was proposed for the liquid crystal alignment process'. It utilizes uni-directional suspension flow over the polyimide layers. The flow induces surface chain orientation and micro-grooves over the threshold stress of 400 Pa., and liquid crystal molecules are aligned to the flow direction. With easy control of operations, the wet rubbing can offer accurate maintenance and surface treatment compared to conventional rubbing
Development of a serum-free medium for dihydrofolate reductase-deficient Chinese hamster ovary cells (DG44) using a statistical design: Beneficial effect of weaning of cells
No full textTo develop serum-free (SF) medium for dihydrofolate reductase-deficient Chinese hamster ovary cells (DG44), a statistical optimization approach based on a Plackett-Burman design was adopted. DG44 cells which were normally maintained in 10% serum medium were gradually weaned to 0.5% serum medium to increase the probability of successful growth in SF medium. A basal medium was prepared by supplementing Dulbeccos modified Eagles medium and Hams nutrient mixture F12 with hypoxanthine (10 mg/l) and thymidine (10 mg/l). Twenty-eight different supplements were selected as variables on the basis of their growth-promoting abilities. From statistical analysis, leucine, tryptophan, lysine, proline, histidine, hydrocortisone, ethanolamine, and phosphatidylcholine were identified as important components showing positive effects on cell growth. A new SF medium (SF-DG44) was formulated by supplementing the basal medium with these components. When the weaned cells were inoculated at 1.0 x 10 cells/ml, a maximum viable cell concentration of 6.4 X 10 cells/ml was achieved in SF-DG44 medium. In contrast, when the unweaned cells were used, a concentration of only 4.1 X 10 cells/ml was reached under the same culture conditions, indicating that weaning of cells improves cell growth in SF medium. In summary, we found that development of a novel SF medium for DG44 cells was facilitated using a Plackett-Burman design technique and weaning of cells
Development of a serum-free medium for the production of humanized antibody from Chinese hamster ovary cells using a statistical design
No full textTo develop serum-free (SF) media for the production of humanized antibody from recombinant Chinese hamster ovary (rCHO cells, a statistical optimization approach based on a Plackett-Burman design was adopted. A basal medium was prepared by supplementing alpha-minimal essential medium (alpha-MEM) with Fe(NO3)(3). 9H(2)O, CuCl2, ZnSO4. 7H(2)O, and Na2SeO3 which are generally contained in SF medium formulations. Insulin, transferrin, and ethanolamine were also supplemented to the basal medium to determine their optimal concentrations. From this statistical analysis, serine, phenylalanine, and tyrosine were identified as important determinants for cell growth. Also, putrescine, linoleic acid, and hydrocortisone were shown to be important for both cell growth and antibody production. The SF medium was formulated by supplementing the basal medium with components showing positive effects on cell growth and/or antibody production. Cell growth and antibody production in this SF medium were comparable to those in alpha-MEM supplemented with 5% dialyzed fetal bovine serum. Taken together, the results obtained here show that a Plackett-Burman design facilitates the development of SF media for rCHO cells aimed at producing a humanized antibody
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