1,082 research outputs found
Practical significance of relative assimilable nitrogen requirements of yeast: a preliminary study of fermentation performance and liberation of H(2)S
No full textThe definitive version is available at www.blackwell-synergy.com Article first published online: 12 MAR 2008Saccharomyces cerevisiae wine yeast strains AWRI 796 and AWRI 835, representing a high and a low nitrogen-requiring strain respectively, were cultured in media of varying nitrogen (ammonium) content. Ammonium utilisation, biomass yield, fermentation progress and the liberation of hydrogen sulfide were monitored. Findings from this study support the original classification of these strains in terms of their relative requirement for nitrogen. Accordingly, when compared with AWRI 835 grown under the same conditions, the high nitrogen-requiring strain, AWRI 796, removed c. 8% more nitrogen from the medium, produced c. 7 to 13% more biomass, failed to complete fermentation in the time frame of the experiment and liberated hydrogen sulfide in greater amounts and/or over longer periods. These findings confirm that measures of relative nitrogen requirement form an extremely useful parameter by which to select strains that are better suited to the problem-free fermentation of musts of low nitrogen content.Jennifer M. Gardner, Kate Poole and Vladimir Jirane
Rapid method for proline determination in grape juice and wine
No full textProline is typically the most abundant amino acid present in grape juice and wine. The amount present is influenced by viticultural and winemaking factors and can be of diagnostic importance. A method for rapid routine quantitation of proline would therefore be of benefit for wine researchers and the industry in general. Colorimetric determination utilizing isatin as a derivatizing agent has previously been applied to plant extracts, biological fluids, and protein hydrolysates. In the current study, this method has been successfully adapted to grape juice and wine and proved to be sensitive to milligram per liter amounts of proline. At sugar concentrations above 60 g/L, interference from the isatin-proline reaction was observed, such that proline concentrations were considerably underestimated in grape juice and dessert wine. However, the method was robust for the analysis of fermentation samples and table wines. Results were within ±10% agreement with data generated from typical HPLC-based analyses. The isatin method is therefore considered suitable for the routine analysis required to support research into the utilization or release of proline by yeast during fermentation.Danfeng Long, Kerry L. Wilkinson, Kate Poole, Dennis K. Taylor, Tristan Warren, Alejandra M. Astorga and Vladimir Jirane
Piire ületamas: Uus Naine Kate Chopini romaanis „Virgumine“
No full textBakalaureusetöö uurimiskeskmeks on 19. sajandi teise poole Ameerika Ühendriikide naisautori Kate Chopini romaan „Virgumine“ (1899), mida analüüsitakse ajastu sotsiaalkultuurilisest kontekstist lähtuvalt. Töös seostatakse Kate Chopini kaasaeg tema loominguga ning näidatakse, kuidas 19. sajandi aktuaalsed diskussioonid naise positsioonist ühiskonnas leiavad kajastust ka „Virgumises“. Konkreetselt keskendutakse Uue Naise (ingl New Woman) fenomenile ning vaadeldakse, kuidas Kate Chopin autorina seda ebakonventsionaalset naisetüüpi käsitleb. Töö eesmärk on analüüsida Uue Naise kui muutuvate soorollide võrdkuju Chopini romaanis „Virgumine“, asetades romaani Uue Naise fenomeni laiemasse kultuurilisse konteksti
Map of the central section of British Columbia : shewing the country served by the Grand Trunk Pacific Railway
No full textScale [ca. 1:6,400] (W 133 00'--W 118 00'/N 56 00'--N 53 00'). Relief shown by hachures. Across top of map: 2,000,000 acres agricultural land
Long-term morphological model of Poole Harbour
No full textThe study focuses on the impact of human interventions on the natural behaviour of estuaries in response to sea-level rise. In particular it focuses on the affect of managed realignment on the critical sea level rise (SLRcrit) for the Poole Harbour. Numerical model ASMITA (Aggregated Scale Morphological Interaction between a Tidal inlet and the Adjacent coast, Stive and Wang, 1996) is used to study the response of the system to the external forcing (both natural and anthropogenic). ASMITA model schematises the tidal-inlet system and characterises each model element by a single variable volume. Volumetric data of the Poole harbour inlet elements obtained through the digitisation of the historical admiralty charts was used to calibrate the model. The model assumes that the each inlet elements tends towards an equilibrium volume which can be defined using the empirical equations. These equilibrium equations consist of equilibrium parameters that depend on the regional properties like hydrodynamics and topography of the Poole harbour. Once the model is calibrated, simulations were performed with various sea level rise scenarios (historic and future SLR) to observe the natural behaviour of the Poole harbour. Managed realignment is being introduced in the model and a range of values of critical sea-level rise was obtained and compared with the predicted sea-level rise rate given in the literature for the next century. Results suggest that the “hold the line” management scenario (current situation) would not result in the complete loss of inter-tidal flats and the coastal flooding is unlikely to occur over the next century, as a result of critical sea-level rise values exceeds the predicted rate. However research suggests, introducing realignment (coastline retreat management scenario) reduces SLRcrit values for the estuary; thus making the estuary more sensitive to the predicted future SLR rates.CoMEMHydraulic EngineeringCivil Engineering and Geoscience
One Hundred Years of Showmanship: Poole's 1837-1937
No full textBooklet describes the Poole family's history as creators of 'panoramas, dioramas, myrioramas, theatres, variety halls, and cinemas.' Illustrations of some of the scenes portrayed, and an explanation of how some of the spectacular effects were produced. Second part consists of a Jim Poole autograph postcard (dated Feburary 1985; addressed to Bill Douglas). Jim Poole was son of the author. Page number(s) as given:
files ending 01_001, 01_001_2, 01_010, 01_010_2, 02, 02_2 are not included in pagination, files ending 002-009_2 are pp.1-1
Coat Cooke & Joe Poole | Coat Cooke & Rainer Wiens: Reviews
No full textCoat Cooke album reviews by Randy Raine-Reusch. Coat Cooke (sax); Joe Poole (drums); Rainer Wiens (guitar)
Proline transport and stress tolerance of ammonia-insensitive mutants of the PUT4-encoded proline-specific permease in yeast
No full textThe imino amino acid, proline, has roles in both cellular nutrition and response to stress. Proline uptake in Saccharomyces cerevisiae is largely mediated by a high affinity, specific permease, Put4p, and a low affinity general amino acid permease, Gap1p. Both are subject to nitrogen catabolite repression (NCR) and nitrogen catabolite inactivation (NCI). In order for proline to be fully exploited, its transport must be derepressed, as occurs upon depletion of preferred nitrogen sources, and molecular oxygen must be present to allow the first step of catabolism via proline oxidase. This study focuses on the isolation of variants of Put4p, which are insensitive to repression by a preferred nitrogen source (ammonia) and their subsequent effect on proline transport and stress tolerance. Specific amino acid residues in the carboxy-terminal region of Put4p were targeted by site-directed mutagenesis. Substitution at Serine(605), a potential phosphorylation target, led to the amelioration of ammonia-induced down-regulation of Put4p. When combined with a promoter mutation (-160), the S(605)A mutation resulted in increased proline uptake and accumulation. This increase in proline accumulation was associated with increased cell viability in conditions of high temperature and osmotic stress raising possible benefits in industrial fermentation applications.Kate Poole, Michelle E. Walker, Tristan Warren, Jennie Gardner, Colin McBryde, Miguel de Barros Lopes and Vladimir Jiranekhttp://www.ncbi.nlm.nih.gov/pubmed/2011860
Jack Chambers : Selection of Paintings and Drawings = Jack Chambers : Sélection de peintures et dessins
No full textPoole discusses Chambers' manner in the painter's own term "perceptual realism". The author notes the growing importance of light in the artist's work. Biographical notes
A policymaker confronts uncertainty
No full textThis article is a reprint of a speech given by William Poole, president of the Federal Reserve Bank of St. Louis, to the St. Louis Gateway Chapter of the National Association for Business Economics. In today's environment economic uncertainties abound both abroad and at home. In this article the author discusses the five categories of uncertainty facing today's policymakers: 1) the data; 2) future events, shocks and disturbances; 3) how the economy works; 4) market reactions to Fed policy; and 5) market anticipations of Fed policy. Poole also discusses the Federal Reserve's role in dealing with each of these uncertainties and today's policy environment.Monetary policy - United States
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