80 research outputs found
Manukau Institute of Technology : an annotated bibliography
No full textManukau Institute of Technology is one of New Zealand's major providers of education in the fields of training and technology. It has progressed to offering degree level programmes related to technology, business, human science and the arts. This annotated bibliography is the first to be compiled, which documents its histoiy, progress, challenges and achievements from its establishment in 1970 to the present. It includes monographs, periodical and newspaper articles, archives, reports, documents and Internet sources. Items are arranged primarily within chronological time periods that correspond to the name changes during its thirty- two-year history. Within each period, items are arranged by material type in alphabetical order. Annotations are descriptive and aim to provide an indication of the content of each item. An appendix listing in chronological order Manukau Institute of Technology's significant events and subject and author indexes are included
The Amino Terminus of Herpes Simplex Virus 1 Glycoprotein K (gK) Is Required for gB Binding to Akt, Release of Intracellular Calcium, and Fusion of the Viral Envelope with Plasma Membranes
No full textABSTRACT
Previously, we have shown that the amino terminus of glycoprotein K (gK) binds to the amino terminus of gB and that deletion of the amino-terminal 38 amino acids of gK prevents herpes simplex virus 1 (HSV-1) infection of mouse trigeminal ganglia after ocular infection and virus entry into neuronal axons. Recently, it has been shown that gB binds to Akt during virus entry and induces Akt phosphorylation and intracellular calcium release. Proximity ligation and two-way immunoprecipitation assays using monoclonal antibodies against gB and Akt-1 phosphorylated at S473 [Akt-1(S473)] confirmed that HSV-1(McKrae) gB interacted with Akt-1(S473) during virus entry into human neuroblastoma (SK-N-SH) cells and induced the release of intracellular calcium. In contrast, the gB specified by HSV-1(McKrae) gKΔ31-68, lacking the amino-terminal 38 amino acids of gK, failed to interact with Akt-1(S473) and induce intracellular calcium release. The Akt inhibitor miltefosine inhibited the entry of McKrae but not the gKΔ31-68 mutant into SK-N-SH cells. Importantly, the entry of the gKΔ31-68 mutant but not McKrae into SK-N-SH cells treated with the endocytosis inhibitors pitstop-2 and dynasore hydrate was significantly inhibited, indicating that McKrae gKΔ31-68 entered via endocytosis. These results suggest that the amino terminus of gK functions to regulate the fusion of the viral envelope with cellular plasma membranes.
IMPORTANCE
HSV-1 glycoprotein B (gB) functions in the fusion of the viral envelope with cellular membranes during virus entry. Herein, we show that a deletion in the amino terminus of glycoprotein K (gK) inhibits gB binding to Akt-1(S473), the release of intracellular calcium, and virus entry via fusion of the viral envelope with cellular plasma membranes.
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Learning at the Workplace: The Call of the Day
No full textIn the present era of modern medical education, the clinicians are realizing the importance and role of workplace learning as compared to the formal classroom teaching. However, multiple practical challenges hamper the process and prevent the optimization of this type of learning. The author has tried to identify the issues and provide suggestions as how all members of the learning team can be prepared to optimize this learning situation. This team includes the student, the tutor, the patient, and an inductive learning environment. The student and tutor should equip themselves with necessary skills and knowledge by reviewing the subject before approaching the patient. The patient should be taken into confidence as their consent, is important. Lastly, the educational environment in the ward should be modified to facilitate learning.Key Words: Learning environment, Medical education, Workplace learningAddres
Cellular and Viral Determinants of HSV-1 Entry and Transport
No full textPreviously, it was shown that the deletion of 38 amino acids from the N terminal end of glycoprotein K (gK) prevents the Herpes Simplex virus Type-1 (HSV-1McKrae ∆gK31-68) from entering into the axons of neurons. Herein, we showed for the first time that this modification in gK disrupts the ability of the virus to carry out a fusion of the viral envelope with the cellular plasma membrane and forces the virus to enter via endocytosis in epithelial cells and neuronal cell bodies. We showed that HSV-1 McKrae infection triggers cellular calcium signaling, Akt activation, and flipping of Akt to the external membrane surface, which facilitates interaction between Akt and HSV-1McKrae glycoprotein B (gB). However, the gK mutant virus fails to trigger calcium signaling and Akt-gB interaction and, therefore, utilizes endocytosis as an alternative to fusion-mediated entry. Following the entry into cells, the HSV-1tegument and capsid proteins utilize cellular motor machinery to mediate capsid transport to the nucleus where the virus replicates its genome. Herein, we showed that the inner tegument protein (UL37) of HSV-1 McKrae interacts with motor protein dynein, and the capsid ICP5 protein interacts with motor accessory proteins, for example, dynactin and EB1. Hence, knocking out selected motor accessory proteins reduced the HSV-1 capsid transport towards the nucleus in human neuroblastoma (SK-N-SH) cells. Interestingly, HSV-1 McKrae altered several cellular regulatory pathways during virus entry and capsid transport. For example, MAP kinases (MAPK) and phosphokinase C (PKC) pathways. Although the virus activates MAPK, this activation does not appear to play essential roles in virus entry and intracellular capsid transport. In contrast, inhibition of PKC inhibited virus entry indicating that this kinase is involved in virus entry and possibly in intracellular capsid transport. The results obtained in these studies suggest that HSV-1 regulates downstream neuronal signaling to facilitate virus entry and intracellular retrograde transport toward the nucleus of infected cells
Pakistan\u27s National Internal Security Policies from 2014 to 2022: Gaps, Challenges, and Prospects
No full textThe National Internal Security Policy (NISP) was a comprehensive policy document aimed at addressing internal security in Pakistan. It was initiated in 2014 and was based on three pillars: Dialogue, Deterrence, and Isolation. The policy was a response to the destruction caused by terrorism and was developed due to Pakistan\u27s role as a front-line state in the US-led Global War on Terror. Between 2004 and 2014, terrorism caused a loss of 50,000 lives and resulted in a 78 billion dollar loss for Pakistan. This study is a critical and comparative assessment of both NISP I and NISP II policies implemented from 2014-2018 and 2018-2022, respectively. The objectives of the study are to assess the effectiveness of Pakistan\u27s security policies and strategies and to identify any alternatives that may have been available. The study will help to evaluate the current approaches to address any weaknesses. The findings of the study could provide useful insights for policy makers in their efforts to improve the country\u27s internal security situation. The study also aims to identify gaps in the policies from a civilian perspective and both qualitative and quantitative research methods were applied.
References
Aziz, K. (2018). Pakistan\u27s National Action Plan: Time for a Reality Check. Journal of Asian Security and International Affairs, 5(1), 111-132.
Government of Pakistan. (2014). National Internal Security Policy 2014-2018. http://www.nacta.gov.pk/storage/nisp2014.pdf
Hussain, K., & Iqbal, M. (2019). Pakistan\u27s Internal Security Policies: An Analysis. Journal of Conflict, Peace and Development, 1(1), 21-30.
Hussain, M. (2017). National security and counter-terrorism in Pakistan: The challenges ahead. Journal of Policing, Intelligence and Counter Terrorism, 12(1), 55-65. https://doi.org/10.1080/18335330.2016.1275717
Hussain, S., Shahzad, F., & Ahmad, S. (2023). A classification framework for analyzing the war and peacemaking potential of News media in Pakistan. Journal of Asian and African Studies, 58(5), 794-811.
Institute for Research, Advocacy, and Development (IRADA). (2019). The Implementation of National Action Plan in Pakistan: A Critical Review. Retrieved from http://www.irada.org.pk/reports/The_Implementation_of_National_Action_Plan_in_Pakistan_A_Critical_Review.pdf
Ismail, M., & Husnain, S. M. (2022). Recalibrating impact of regional actors on security of China–Pakistan Economic Corridor (CPEC). Fudan Journal of the Humanities and Social Sciences, 15(3), 437-462.
Janjua, R. A. (2019). Security challenges and opportunities in Pakistan: A critical analysis. Strategic Studies Quarterly, 13(1), 120-136. https://doi.org/10.1080/09700161.2019.1577483
Khan, A. (2016). Pakistan’s National Action Plan against terrorism: An appraisal. Journal of Policing, Intelligence and Counter Terrorism, 11(2), 110-121. https://doi.org/10.1080/18335330.2016.1183701
Khan, M. H. (2021). Pakistan\u27s Internal Security: Challenges and Prospects. Journal of the Research Society of Pakistan, 58(1), 207-221.
Niazi, A. U. (2020). National security policy of Pakistan: A critical analysis. Pakistan Journal of International Affairs, 31(2), 63-78. https://doi.org/10.36172/pjia.v31i2.1153
Pakistan Institute of Peace Studies (PIPS). (2015). National Action Plan: An Overview. Retrieved from http://san-pips.com/PIPS-Research-Reports/National-Action-Plan.pdf
Warraich, H. U. (2017). Pakistan\u27s national security policy: An evaluation. Journal of Political Studies, 24(2), 39-56. https://www.jstor.org/stable/2646631
Impact of psychological capital on organizational citizenship behavior
No full text
Purpose
The purpose of this paper is to examine the mediating role of work engagement (WE) between psychological capital (PsyCap) and the two facets of organizational citizenship behavior (OCB) which involves both individual and organization. It also examines the moderating role of perceived organizational support (POS) between PsyCap and WE, and WE and the two facets of OCB.
Design/methodology/approach
A total of 293 responses gathered from employees working in diverse service sector industries in India were assessed using structural equation modeling.
Findings
Overall, the results support the mediating role of WE in the PsyCap-OCB relationship, and the moderation of POS between WE and the two facets of OCB.
Research limitations/implications
This study helps in understanding how WE-OCB relationship can be negatively affected in the presence of high POS.
Practical implications
The results encourage organizations to establish systems for enhancing the engagement levels of their employees, which according to this study may be achieved by creating and maintaining vibrant work environment.
Originality/value
This study helps in understanding the role of POS among PsyCap, WE, \and the two factors of OCB.
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Stylistic Study of Adjectives in How to Get Filthy Rich in Rising Asia
No full textThe present research explores the choice of adjectives as a lexical category in Mohsin Hamid’s novel, How to Get Filthy Rich in Rising Asia by using Leech and Short model (1981). An empirical enquiry is carried out to trace the author’s choice of adjectives and their intended functions by subjecting How to Get Filthy Rich in Rising Asia to stylistic analysis and linguistic scrutiny. The various functions of adjectives are interpreted after the text is subjected to close reading for their contextual occurrence where they are carefully engraved by the author. The resultant functions throw ample light on the life, culture, economic scenario and love and gender relations construed in the text through adjectives. The present paper, however, is limited only to the interpretation of the adjective categories based on the model suggested by Leech & Short (1981). This study is, therefore, instrumental in initiating a voyage to interpret literary language via linguistic tools and evidences contributing amply to the field of stylistics as well as literary criticism. </jats:p
Magnetic Nanoparticles: A Review on Stratagems of Fabrication an d its Biomedical Applications
No full textNur-i-Afshan V.63 no.26 December 1935
No full textContents: G̲h̲azal : maulūd muqadas [Poetry] by Z̲ākir Meraṭhī - Aiḍīṭoriyal : ṣūlaḥ ke shahzāde - Carc yūniyan [Article] by Pādrī Jalāluddīn - Ishtihār-i kutab : baccoṉ kī kitāben̲ [News] - Kalām mujassam huvā [Article] by Pādrī Jalāluddīn - Āʼiye baṛe din ke liʼe ghar sajā'īn̲ [Article] by ʻIbādulláh, Ae. - Vilādat-i Masīḥ : āʼiye ham ghar sajā'īn̲ [Article] by ̒Alī Bak̲h̲sh, Je. - ʻAjīb pedā’ish [Article] by Minat, Ke. En. - Qādiyānun kī Masīḥīyūn̲ ke hāthon̲ taz̲līl [News] - Lut̤f-i suk̲h̲an : mas̲man dar musarrat ʻīd-i vilādat [Poetry] - G̲h̲azal : maulūd-i aqdas [Poetry] by Z̲ākir Meraṭhī - Baṛā din [Poetry] by Minat, Ke. En. - Shuʻāʻ-i ʻirfān [Poetry] - Baṛā din mubārak ho [Poetry] by Madan, Viliyam Mīsī - Talāsh-i ḥaqq [Article] - Shahanshāh kaunaīn : idhar āʼo ! mere ajmāl kī tafṣīl ko dekho [Article] by Durrānī, Em. Ec. - Gurū Nānak kī taʻlīm [Article] by Rāʼbarn, Ḍabliyu. Em. - Ibn-i K̲h̲udā āyā he [Poetry] by Durrānī, Em. Ec. Afsānah : baṛe din kā inʻām [Fiction] by Mīsī, Masīḥ Dās - Kriscan hā’ī skūl kharaṛ Z̤ilaʻ Ambālah [News]
This volume of Nur-i-Afshan published bi-weekly, on alternate Fridays from Kharar District Ambala
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