1,721,634 research outputs found
BIBLIOGRAPHY OF ACADEMICIAN GLIGOR JOVANOVSKI
No full textBIBLIOGRAPHY OF ACADEMICIAN GLIGOR JOVANOVSKI</jats:p
El lenguaje universal de la poesía
No full textBranje prevodov na literarnem večeru, moderiranje in tolmačenje pogovora s pesnikoma Gašperjem Malejem in Alenko Jovanovski
El diagrama como el redefinidor del tipo : Aeropuerto en La Plata
No full textTrabajo de tesis de Iván Jovanovski. El proyecto es un aeropuerto para el cual se realiza un estudio de edificios históricos de la ciudad de La Plata para el tipo de lenguaje arquitectónicos de la ciudad; y un estudio de aeropuertos para definir el tipo funcional que define las relaciones espaciales necesarias para el correcto funcionamiento del edificio. Además se realiza una serie de diagramas, los cuales permiten redefinir los tipos anteriormente planteados.ArquitectoUniversidad Nacional de La PlataFacultad de Arquitectura y Urbanism
Vplivi okoljskih pogojev na kvaliteto in fizikalno-kemijske lastnosti biološko proizvedenih polihidroksialkanoatov (PHA)
No full textImpacts of selected environmental conditions on the quality and quantity of polyhydroxyalkanoates (PHAs) produced with surplus activated sludge were studied. The effects on the PHA-rich biomass were measured immediately after the pH adjustments in the range from pH 2 to pH 12, and after a 48-h anaerobic incubation at 55 °C without prior PHA extraction from biomass. For comparison, two control samples at native pH, incubated at 55 °C and 25 °C, were studied. Methods applied involved quantification of the PHA by thermogravimetric analysis (TGA) together with volatile suspended solids (VSS) or chemical oxygen demand (COD) analysis. Degradation products were measured by ion chromatography (IC) or ultra-high-pressure liquid chromatography (UHPLC). PHA thermal decomposition behaviour and typical phase transitions were analysed by TGA and differential scanning calorimetry (DSC), respectively. Results show that PHA properties (quantity and quality) were best preserved at pH 2. Other conditions revealed different degrees of PHA degradation, where neutral, alkaline conditions and incubation at 25 °C were the most detrimental, especially after 48 h of incubation – a PHA concentration decrease of up to (56 ± 5)% was observed. The acidification of samples before the measurements improved their thermal stability. The observed decrease of measurable PHA mass at pH > 2 was accompanied by the changes in thermal properties (melting, crystallization, glass transition) that were indicative of decreased stability or integrity of PHA and can be explained by a decrease in the molecular weight of PHA.S polihidroksialkanoati (PHA) bogato biomaso, smo izpostavili izbranim dejavnikom okolja. Namen je bil raziskati njihov vpliv na količino in nekatere fizikalno-kemijske lastnosti PHA, proizvedenih z dohranjevanjem odpadnega aktivnega blata. Vzorce smo analizirali brez predhodne izolacije PHA iz biomase. S PHA bogato biomaso smo med 48-urno anaerobno inkubacijo izpostavili različnim pH pogojem (med pH = 2 in pH = 12) ter povišani temperaturi (55 °C). Vzorce smo analizirali takoj po spremembi pH pogojev in še po 48-urni inkubaciji. Dve kontrolni skupini, ki jim pH pogojev nismo spreminjali, inkubirani pri 25 °C in 55 °C, sta nam služili za primerjavo. Za kvantifikacijo PHA v biomasi smo uporabili termogravimetrično analizo (TGA) podprto z določanjem koncentracije organske snovi in kemijske potrebe po kisiku (KPK). Razgradni produkti v topni fazi so bili določeni z ionsko kromatografijo (IC) ali ultra visokotlačno tekočinsko kromatografijo (UHPLC). Termično razgradnjo smo analizirali s TGA, fazne prehode PHA pa z diferenčno dinamično kalorimetrijo (DSC). Količina in fizikalno-kemijske lastnosti PHA so se najmanj spremenile v kislih pogojih (pH = 2). Pri ostalih pogojih smo opazili različne stopnje razgradnje PHA. Nevtralni in alkalni pogoji ter inkubacija na 25 °C so povzročili največjo razgradnjo PHA, tj. za (56 ± 5) %. Nakisanje vzorcev pred termičnimi analizami je izboljšalo njihovo termostabilnost. Zmanjšanje koncentracije PHA pri pogojih pH > 2 smo povezali tudi s spremembami v faznih prehodih (kristalizacija, taljenje, steklasti prehod), kar nakazuje na zmanjšano stabilnost ali integriteto PHA, kar bi lahko razložili z zmanjšanjem molekulske mase PHA
Testing molecular glues for controlled aggregation of Saccharomyces cerevisiae yeast cells
No full textMikrokapsulacija mikrobnih celic je privlačna tehnika za mnogo aplikacij, saj lahko s
takšno nadzorovano agregacijo zaščitimo celice pred inhibitorji iz okolja, povečamo
produktivnost procesa, olajšamo ponovno uporabo celic ali zagotovimo zaščito in
sproščanje celic v zaželenih pogojih. V magistrski nalogi smo preučili metodo
koacervacije polisaharidov za sočasno agregacijo celic kvasovk Saccharomyces
cerevisiae. Kot glavni nosilni polimer smo izbrali hitozan, v kombinaciji s hitozanom
pa smo testirali tudi dodatne komponente: (i) ciklodekstrine (CD) – α, β, γ, in/ali (ii)
alginat v različnih koncentracijah. Opazovali smo oblikovanje velikosti agregatov skozi
čas ter odstotek celic, ki so se ujele v agregate oziroma, ki so ostale proste. Dognali smo,
da različni testirani CD niso bistveno vplivali na agregacijo in velikost delcev, medtem
ko smo z različnimi koncentracijami alginata opazno vplivali na pridobljene agregate.
V vseh vzorcih so nastali agregati velikosti med 50 in 100 μm, z izjemo vzorca, ki je
vseboval hitozan (1 mg/mL) in alginat (1 mg/mL), kjer so nastali agregati večji od 1
mm. Največji odstotek celic (60,7 %) smo ujeli v agregate s hitozanom (1 mg/mL) in
alginatom (0,5 mg/mL), najmanj (25,4 %) pa v vzorcu s hitozanom (1 mg/mL) in
alginatom (0,25 mg/mL). Izmed CD se je pri uspešnosti vezave celic v agregate najbolje
(56,8 %) odrezal γ-CD, vrednosti v prisotnosti α-CD (40,8 %) in β-CD (43,6 %) pa so
bile nekoliko manjše. V nalogi smo spremljali tudi viabilnost v agregate ujetih celic,
kjer smo po 24 urah v vzorcih določili odstotek viabilnih celic med 77,5 % in 92,9 %.
Razvita metoda za nadzorovano agregacijo S. cerevisiae je enostavna, hitra in hkrati ne
zahteva dragih namenskih naprav ali tehnično zahtevne izvedbe ter je kot taka primerna
tudi za izvedbo v aseptičnih pogojih.Microencapsulation of microbial cells is an attractive method for many applications.
Controlled aggregation can protect cells from environmental inhibitors, increase the
productivity of the process, facilitate the reuse of cells, or ensure the protection and
release of cells under desired conditions. In this master\u27s thesis, we studied the
polysaccharide coacervation method for simultaneous aggregation of Saccharomyces
cerevisiae yeast cells. We chose chitosan as the main carrier polymer, and in
combination with chitosan we also tested additional components: (i) cyclodextrins (CD)
– α, β, γ, and/or (ii) alginate in different concentrations. We observed the formation of
aggregate size over time and the percentage of cells that were entrapped in aggregates
or that remained free. We concluded that the different tested CDs did not significantly
affect the aggregation and particle size, while different concentrations of alginate
significantly affected the obtained aggregates. Aggregates between 50 and 100 μm in
size were formed in all samples, with the exception of the sample containing chitosan
(1 mg/mL) and alginate (1 mg/mL), where aggregates larger than 1 mm were formed.
The highest percentage of cells (60.7%) was captured in aggregates with chitosan (1
mg/mL) and alginate (0.5 mg/mL), and the lowest (25.4 %) in the sample with chitosan
(1 mg/mL) and alginate (0.25 mg/mL). Among the CDs, γ-CD performed best (56.8%)
in the success of binding cells into aggregates, while the values in the presence of α-CD
(40.8%) and β-CD (43.6%) were slightly lower. In the thesis, we also monitored the
viability of cells entrapped in aggregates. After 24 hours the percentage of viable cells
was between 77.5% and 92.9% for all samples. The developed method for the controlled
aggregation of S. cerevisiae is simple, fast and, at the same time, does not require
expensive machines or technically demanding implementation, and as such is also
suitable for implementation in aseptic conditions
Eksperimentalna analiza kavitacijske erozije v toku kapljevine s trdimi delci
No full textCavitation erosion is a problem that presents a challenge for the engineers in different industries, as it erodes the machinery which leads to lower efficiency and higher maintanance costs. An experimental investigation has been conducted to evaluate cavitation and cavitation erosion with and without the presence of solid particles in a Venturi channel. The methodology is based on observing and analysing the evolution of the vapour structures and occurence of cavitation erosion on the sample surface in the Venturi channel. To conduct experiments the cavitation tunnel was used, with the central component being a transparent test section with a Venturi-like channel (constructed from Plexiglass walls and a metal insert) to monitor the process of cavitation. To detect the damage caused by cavitation erosion and abrasion, the most effective solution was found to be an indicator in the form of black acrylic paint thinned with water (at a ratio of 1:2). On the sample surface, a self-adhesive aluminium tape was applied to protect the aluminium base from erosive characteristics from cavitation Furthermore, a comparison between cavitation with and without solid particles was made to determine the effects of solid particles on both cavitation and abrasion of the sample surface. The observation revealed that no matter the solid particles, damage from cavitation erosion appears at the separation point and in proximity to the location of the detached cloud collapse. The spherical glass particles with diameters between 40 µm and 70 µm added an abrasive effect on the sample surface only in the presence of cavitation structures. It was determined, that the intensity of cavitation erosion and abrasion increases when the cavitation number decreases and volumetric flow rate increases. Also, an economic analysis was conducted, to determine which is the more cost beneficial solution between replacement of the metal insert and application of protective coatings over the metal insert.Kavitacijska erozija je izziv za inženirje v različnih industrijah, saj povzroča erozijo strojev, kar vodi do manjše učinkovitosti in višjih stroškov vzdrževanja. Izvedena je bila eksperimentalna analiza kavitacijske erozije v toku kapljevine s trdnimi delci v Venturijevem kanalu in brez njih. Metodologija temelji na analizi in opazovanju razvoja parnih struktur in kavitacijske erozije na površini vzorca v Venturijevem kanalu. Za izvedbo eksperimentov je bil uporabljen kavitacijski tunel, katerega osrednji del je bil prozoren testni odsek z Venturijevim kanalom (izdelanim iz sten iz pleksi stekla in kovinskega vstavka) za spremljanje procesa kavitacije. Za spremljanje poškodb, ki jih povzročata kavitacijska erozija in abrazija, se je kot najučinkovitejša rešitev izkazal pokazatelj v obliki črne akrilne barve, razredčene z vodo (v razmerju 1 : 2). Na površini vzorca je bil nameščen samolepilni aluminijasti trak za zaščito aluminijaste podlage pred erozijskimi lastnostmi zaradi kavitacije. Poleg tega je bila izvedena primerjava med kavitacijo s trdnimi delci in brez njih, da bi ugotovili učinke trdnih delcev na kavitacijo in
površino vstavka. Opazovanja so pokazala, da se ne glede na trdne delce, poškodbe zaradi kavitacijske erozije pojavijo na mestu separacije kavitacijskega oblaka in in v bližini kolpasa kavitacijskega oblaka. Krogelni, stekleni delci premera od 40 µm do 70 µm so zgolj ob prisotnosti kavitacijskih struktur dodali abrazivni učinek na površino vstavka. Opazili smo, da se intenzivnost kavitacijske erozije in abrazije poveča, ko se kavitacijsko število zmanjša, volumski pretok pa poveča. Opravljena je bila tudi ekonomska analiza, da bi ugotovili, katera rešitev je stroškovno ugodnejša med zamenjavo kovinskega vstavka in nanosom zaščitnih premazov na kovinski vstavek
Design of fast responsive signaling pathways in mammalian cells
No full textSintetična biološka logična vezja, podobno kot električna, zaznavajo vstopne informacije, jih predelajo ter na podlagi le-teh tvorijo specifične celične odzive. Večina do sedaj ustvarjenih logičnih vezij temelji na regulaciji transkripcije, ki je časovno potratnejša kot prenos signala preko proteinskih interakcij. Za zdravljenje določenih bolezni, kot je diabetes, je regulacija transkripcije prepočasna, saj za razvoj polnega signala potrebuje nekaj ur. V diplomski nalogi predstavljamo umetno ustvarjeno hitro odzivno signalno pot, ki je modularna ter med aplikacijami lahko prenosljiva. V HEK293T celice smo vnesli na obvitih vijačnicah ter proteolizi osnovanih konstruktih temelječ sistem za logično procesiranje signala. Sistem je inducibilen z rapamicinom ter abscizinsko kislino, vendar bi se ga dalo prilagoditi, da se odziva na eksogene signale kot sta ultrazvok ter svetloba, ali na endogene signale. Testirali smo logično funkcijo IN, preko katere smo le ob vnosu obeh izbranih vstopnih signalov hkrati dobili željen signal že 10 minut po stimulaciji celic. S prilagoditvijo cepitvenih mest, lahko s takšnim sistemom izvajamo tudi ostale Boolove logične funkcije, kar ustvarjenemu sistemu omogoča še večjo uporabnost na različnih znanstvenih področjih.Much like electrical circuits, synthetic biological logic circuits have the ability to sense inputs, process them and form celullar responses based on inputs. So far, most logic circuits are based on regulating transcription, where signal transduction is more time consuming than signal transduction through protein-protein interactions. For treating certain illnesses like diabetes, transcription regulation is too slow, as signals are fully developed after a few hours. This Bachelor thesis presents a synthetical fast responsive signalling pathway, which is highly modular and thus very easily transferable between different applications. We have tranfected HEK293T cells with constructs based on coiled coils and proteolysis for logical processing of signals. The system is inducible with rapamycin and abscisic acid but it can be modified to cell reaction to other exogenous signals like ultrasound and light or to cellular endogenous signals. We have demonstrated the Boolean function AND, through which the desired signal was obtained 10 minutes after stimulating cells only with both input signals. By adjusting the cleavage sites, the system is able to execute other Boolean functions as well, thus ensuring the system an even greater possibility of interdisciplinary use
Stressed skin design of single storey steel buildings
No full textMaster`s thesis addresses problem of stressed skin design of one storey steel buildings. The first part deals with different structural systems, where stressed skin design is used to provide the horizontal stiffness. The stiffness is provided by the profiled sheeting that is the main structural element in the roof structure. There are two cases described. The first one, where the profile sheeting is fastened to the rafter and second one, where the sheeting is fastened to the purlins. Further on, the details are described that are relevant for diaphragm bearing capacity. The second part deals with an example of single storey steel building, where the stressed skin design is performed. The plan of the building is 20 by 45 meters. Ten frames are placed in the short direction. The stressed skin design is described according to publication ECCS (ECCS, 1995) and according to Höglund (Höglund, 2002). The profiled sheeting was also used as the support to prevent lateral buckling of the rafters. The design of fasteners and the interaction between main frames and profiled sheeting is presented. The objective was to achieve low weight of the structure
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