3 research outputs found
Transformation of Tropical Maize with the NPK1 Gene for Drought Tolerance
No full textAbstract The objective of this study was to determine amenability of different tropical maize genotypes to Agrobacterium-mediated transformation with the NPK1 gene for conference of drought tolerance. To achieve this, immature embryos fro m thirteen maize genotypes (IL1, IL3, IL15, IL16, IL28, IL38, IL42, IL43, Hudiba-1, Hudiba-2, Mojtamma-45, A188 and KAT) were transformed by cocultivating with Agrobacterium tumefaciens strain EHA101 harboring the pSHX004 vector. Transgenic tussues were recovered on PPT (3 mg/l)-containing mediu m. Statistically significant differences (p<0.05) were observed between the genotypes with respect to transformation frequency (TF). Overall, IL3 was identified as the most amenable to transformat ion with a TF of 31.7% and proved to be superior to A188, which recorded a TF of 5.82%. Hudiba-2 was identified as the most transformable open pollinated variety (OPV) with a TF o f 8.7% co mpared to that of 7.3% for KAT. IL1 and Mojtamma-45 proved to be poor responders to transformation with TFs of 2.5% and 1.7%, respectively. Putative transgenics were recovered fro m IL3, IL15, Hudiba-2, IL1, IL38, Hudiba-1, A188 and KAT. The frequency of regeneration of PPT resistant shoots varied from 6% for A 188 to 100% fo r Hudiba-2. Stable integration of the transgene was confirmed by PCR. In conclusion we have demonstrated that tropical maize genotypes adapted to Sudan are transformab le with the NPK1 gene
