429 research outputs found
Jefferson Medical College of Philadelphia. Lectures on Institutes of Medicine &c. By Professor Dunglison. Admit Mr. John Flickinger
No full textSession 1848-1849https://jdc.jefferson.edu/lecturetickets/1719/thumbnail.jp
Jefferson Medical College of Philadelphia. Lectures on Institutes of Medicine &c. By Professor Dunglison. Admit Mr. John Flickinger (verso)
No full textSession 1848-1849https://jdc.jefferson.edu/lecturetickets/1720/thumbnail.jp
Football Team (1892)
No full textJump, H. D.; Bowers, John C.; Albert, John J.; Nicholas, J. Crayton; Flickinger, Frank E.; Kloss, Charles F.; Enders, George W.; Rudisill, Andrew J.; Kump, William A.; Hipsley, George E.; Fair, John S.; Kline, M. J.; Duttera, W. B.; Moser, I. O.; Vastine, W. M.;
Koller, P. W.; Keefer, William B.; Bastian, C. P.; Menges, W.; Witman, H. M.;Spectrum 1894, p. 139; Tipton #138
Guanylyl Cyclase C Vaccines for Secondary Prevention of Metastatic Colorectal Cancer
No full textOver the last decade, it has become evident that the immune system can be leveraged in the fight against cancer. Indeed, engaging the immune system through immune checkpoint inhibitor therapy has generated unprecedented results and revolutionized cancer drug development. However, effective therapies for many cancers, such as metastatic colorectal cancer (CRC), are still lacking. In this context, we have developed a vaccine against metastatic CRC for individuals with a high-risk of developing this disease. This vaccine targets the tumor-associated antigen guanylyl cyclase c (GUCY2C, GCC), an antigen that is near universally expressed in CRC. A phase I clinical trial testing an adenovirus-based GUCY2C vaccine (Ad5-GUCY2C-PADRE) recently demonstrated the ability to safely induce GUCY2C-specific immune responses in patients. While promising, this trial also indicated that the vaccine is hindered in two capacities. The trial showed that 1) some patients possess pre-existing antibodies that neutralize the adenovirus vaccine vector and prevent the induction of GUCY2C-specific immune responses and 2) many patients fail to mount CD4+ T-cell responses to the CD4+ helper T-cell epitope, PADRE, which is necessary for effective GUCY2C-specific immune responses. Here, we evaluate a novel GUCY2C immunization regimen utilizing the chimeric adenovirus Ad5.F35 and the bacterium Listeria monocytogenes (Lm) as vaccine vectors. We demonstrate that this combination induces potent antitumor immunity and is not limited by pre-existing Ad5 or Lm immunity. Moreover, through defining the utility of this vaccination regimen, we uncover novel findings concerning the role of CD4+ helper T-cells during CD8+ T-cell responses and reveal limitations of the popular vaccine vector, Lm. These studies carry implications for the design of GUCY2C vaccines as well as designs for vaccines targeting other cancer or infectious disease antigens
Upstream industrial biotechnology: Expression systems and process development. Volume 1.
No full textHoboken, NJxvii, 786 p.: bibl. ref., index; 28 c
Downstream industrial biotechnology: Recovery and purification
No full textHoboken, NJxiv, 858 p.: bibl. ref., index; 28 c
Baculovirus expression systems
No full textBaculoviruses are insect-specific pathogens, producing occlusion bodies containing enveloped virus particles which spread infection between insects. These occlusions or polyhedra are composed mainly of a 30 KDa virus-encoded protein produced in the very late phase of virus replication. Polyhedrin protein is not required for virus replication in cell culture and so the gene coding region can be replaced with a foreign sequence of choice to generate a baculovirus expression vector. The inserted coding region is placed under the transcriptional control of the polyhedrin gene promoter by transfection of insect cells with circular or linear baculovirus DNA or via transposition in bacteria. The coding region can be modified to allow the addition of different secretion signal peptides or peptide tags or multiple gene promoters can be used for the expression of more than one protein. The baculovirus expression vector may also be modified by the removal of nonessential genes encoding products deleterious to recombinant protein production. High yields of protein are possible by scaling up insect cell cultures in serum-free medium. Despite their specificity for insect cells, baculoviruses can also be use to transduce human cells, where recombinant genes can be expressed if placed under the control of mammalian-specific gene promoters
C-structures and f-structures for the British national corpus
No full textWe describe how the British National Corpus (BNC), a one hundred million word balanced corpus of British English, was parsed into Lexical Functional Grammar (LFG) c-structures and f-structures, using a treebank-based
parsing architecture. The parsing architecture uses a state-of-the-art statistical parser and reranker trained on the Penn Treebank to produce context-free phrase structure trees, and an annotation algorithm to automatically annotate
these trees into LFG f-structures. We describe the pre-processing steps which were taken to accommodate the differences between the Penn Treebank and the BNC. Some of the issues encountered in applying the parsing
architecture on such a large scale are discussed. The process of annotating a gold standard set of 1,000 parse trees is described. We present evaluation results obtained by evaluating the c-structures produced by the statistical parser against the c-structure gold standard. We also present the results obtained by evaluating the f-structures produced by the annotation algorithm against an
automatically constructed f-structure gold standard. The c-structures achieve an f-score of 83.7% and the f-structures an f-score of 91.2%
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