608 research outputs found
sj-docx-1-jef-10.1177_15589250231220478 – Supplemental material for Screen-printed fabric electrode for electrocardiogram monitoring on commercial textiles
No full textSupplemental material, sj-docx-1-jef-10.1177_15589250231220478 for Screen-printed fabric electrode for electrocardiogram monitoring on commercial textiles by Jiarui Jin, Wang Jia and Xuemei Yang in Journal of Engineered Fibers and Fabrics</p
sj-pdf-1-jcb-10.1177_0271678X221135419 - Supplemental material for An enriched environment improves long-term functional outcomes in mice after intracerebral hemorrhage by mechanisms that involve the Nrf2/BDNF/glutaminase pathway
No full textSupplemental material, sj-pdf-1-jcb-10.1177_0271678X221135419 for An enriched environment improves long-term functional outcomes in mice after intracerebral hemorrhage by mechanisms that involve the Nrf2/BDNF/glutaminase pathway by Peijun Jia, Junmin Wang, Xiuhua Ren, Jinxin He, Shaoshuai Wang, Yinpei Xing, Danyang Chen, Xinling Zhang, Siqi Zhou, Xi Liu, Shangchen Yu, Zefu Li, Chao Jiang, Weidong Zang, Xuemei Chen and Jian Wang in Journal of Cerebral Blood Flow & Metabolism</p
Single-cell RNA sequencing in juvenile idiopathic arthritis
No full textJuvenile idiopathic arthritis (JIA) is one of the most common chronic inflammatory rheumatic diseases in children, with onset before age 16 and lasting for more than 6 weeks. JIA is a highly heterogeneous condition with various consequences for health and quality of life. For some JIA patients, early detection and intervention remain challenging. As a result, further investigation of the complex and unknown mechanisms underlying JIA is required. Advances in technology now allow us to describe the biological heterogeneity and function of individual cell populations in JIA. Through this review, we hope to provide novel ideas and potential targets for the diagnosis and treatment of JIA by summarizing the current findings of single-cell RNA sequencing studies and understanding how the major cell subsets drive JIA pathogenesis
Recommended from our members
A Study of the MOS4-Associated Complex in RNA Metabolism in Arabidopsis
No full textIn Arabidopsis thaliana, the MOS4-ASSOCIATED COMPLEX (MAC) is required for defense and development. The evolutionarily conserved putative RNA helicase MAC7 is a component of the Arabidopsis MAC, and the human MAC7 homolog, Aquarius, is implicated in precursor messenger RNA (pre-mRNA) splicing. In my thesis research, I studied the functions of MAC in RNA metabolism and other aspects of Arabidopsis development and defense. First, we show that the partial loss-of-function mutant mac7-1 and two other MAC subunit mutants, mac3a mac3b and pleiotropic regulatory locus 1 pleiotropic regulatory locus 2 (prl1 prl2), exhibit reduced microRNA (miRNA) levels, indicating that MAC promotes miRNA biogenesis. The mac7-1 mutant shows reduced primary miRNA (pri-miRNA) levels without affecting miRNA gene (MIR) promoter activity or the half-life of pri-miRNA transcripts. As a nuclear protein, MAC7 is not concentrated in dicing bodies, but it affects the localization of HYPONASTIC LEAVES1 (HYL1), a key protein in pri-miRNA processing, to dicing bodies. Immunoprecipitation of HYL1 retrieved eleven known MAC subunits, including MAC7, indicating association between HYL1 and MAC. We propose that MAC7 links MIR transcription to pri-miRNA processing. mRNA-seq analysis showed that down-regulated genes in MAC subunit mutants are mostly involved in plant defense and stimulus responses, confirming a role of MAC in biotic and abiotic stress responses. We also discovered global intron retention defects in mutants of three subunits of MAC, thus linking MAC function to mRNA splicing in Arabidopsis. Second, since previous studies showed that MAC has multiple functions in eukaryotes, including roles in transcription elongation, mRNA splicing, DNA repair, RNA export and the ubiquitin-proteasome pathway, we explored other potential functions of Arabidopsis MAC and the MAC7 subunit in particular beyond miRNA biogenesis and pre-mRNA splicing. We found that MAC7 has a potential role in photosynthetic pathways, as RNA-seq analysis showed that many photosynthesis-related genes are down-regulated in this mutant. In addition, MAC7 and PRL proteins are very likely to be involved in ribosomal RNA (rRNA) biogenesis, as many rRNA precursors accumulated in the mac7-1 and prl1 prl2 mutants
CYP72A enzymes catalyse 13-hydrolyzation of gibberellins
No full textBioactive gibberellins (GAs, diterpenes) are essential hormones in land plants, controlling many aspects of plant growth and developments. In flowering plants, 13-OH (low bioactivity; such as GA1) and 13-H GAs (high bioactivity; such as GA4) frequently coexist. However, the bona fide GA 13-hydroxylase and its physiological functions in Arabidopsis remain unknown. Here, we report that novel cytochrome P450 genes (CYP72A9 and its homologs) encode active GA 13- hydroxylases in Brassicaceae plants. CYP72A9-overexpressing plants exhibited semi-dwarfism, which was caused by significant reduction in GA4 levels. Biochemical assays revealed that recombinant CYP72A9 protein catalyzed the conversion from 13-H GAs to the corresponding 13-OH GAs. CYP72A9 was expressed predominantly in developing seeds in Arabidopsis. Freshly harvested seeds of cyp72a9 mutants germinated more quickly than wild-type, while long-term storage and stratification-treated seeds did not. The evolutionary origin of GA 13- oxidases from the CYP72A subfamily also was investigated and discussed here.This is a manuscript of an article published as He, Juan, Qingwen Chen, Peiyong Xin, Jia Yuan, Yihua Ma, Xuemei Wang, Meimei Xu, Jinfang Chu, Reuben J. Peters, and Guodong Wang. "CYP72A enzymes catalyse 13-hydrolyzation of gibberellins." Nature plants (2019). doi: 10.1038/s41477-019-0511-z. Posted with permission.</p
Promalactis subclavata Du, Wang et Li 2014
No full textPromalactis subclavata Du, Wang et Li, 2014 (Fig. 17) Promalactis subclavata Du, Wang et Li, 2014: 103. TL: China (Taiwan). TD: MNHU. Material examiNed. Type material. CHINA: Holotype ♂, Huisun Exp. Forest (24.08°N, 121.33°E), Nantou, Taiwan, 1100 m, 22−24.IV.1999, coll. W. Mey and K. Ebert, slide No. MNHUNK056. Paratypes: 2♀, same data as holotype, slide Nos. DZH09005 ♀, DZH10127♀; 1♂, Wuzhishan Reserves, Hainan, 740 m, 14.IV.2009, coll. Qing Jin and Bingbing Hu; 1♂, Yinggeling, Hainan, 620 m, 6.V.2010, coll. Bingbing Hu and Jing Zhang. AdditioNal material. HaiNaN: Wuzhishan Nature Reserves: 2♂, 710 m, 20−21.IV.2014, coll. Tengteng Liu, Wei Guan and Xuemei Hu, slide No. HS 14069 ♂, 1♂, 742 m, 4.VII.2014, coll. Peixin Cong, Linjie Liu and Sha Hu, slide No. HS 14068 ♂, 1♂, 20.V.2014, coll. Peixin Cong, Wei Guan and Sha Hu, slide No. HS 15155 ♂, 4♂, 738 m, 24,27.VII, 29.X, 2.XI.2016, coll. Xia Bai, Shuonan Qian and Wanding Qi, slide Nos. JYY17084 ♂, JYY17144♂; Lizudadian, Shuiman, Mt. Wuzhi: 3♂, 766 m, 5−7.VII.2015, coll. Qingyun Wang, Suran Li and Mengting Chen, slide Nos. HS15182 ♂, HS14141 ♂, 6♂, 2♀, 766 m, 31.VII‒3.VIII.2016, coll. Xia Bai, Shuonan Qian and Waning Qi, slide Nos. JYY17086 ♂, JYY17087♀, 640 m, 28.X.2016, coll. Xia Bai, Shuonan Qian and Wanding Qi; 1♀, Tianchi, Jianfengling, 787 m, 5.VIII.2016, coll. Xia Bai, Shuonan Qian and Wanding Qi, slide No. JYY17143 ♀. DescriptioN of female. Wing pattern and color same as male. Female genitalia (Fig. 17). Apophyses anteriores about 4/5 length of apophyses posteriores. Ostium bursae in broad V-shape, with narrow sclerotized lateral edge, joined anteriorly and produced roundly. Ductus bursae with basal 3/4 uniform and sclerotized, distal 1/4 dilated and membranous, with three large spines before end of sclerotized part. Corpus bursae ovate, with two large round signa, placed anteriorly. DistributioN. China (Hainan, Taiwan). Notes. The female of this species is described for the first time.Published as part of Wang, Shuxia & Jia, Yanyan, 2017, Review of the genus Promalactis (Lepidoptera: Oecophoridae) Meyrick, 1908 (II). The suzukiella group, with descriptions of eight new species, pp. 361-376 in Zootaxa 4363 (3) on page 366, DOI: 10.11646/zootaxa.4363.3.3, http://zenodo.org/record/110806
Evaluating the causal effect of circulating proteome on the risk of Juvenile idiopathic arthritis: an omics pipeline study
No full textAbstract Background Genome-wide association studies (GWAS) have pinpointed a multitude of risk loci associated with Juvenile Idiopathic Arthritis (JIA), but it is challenging to decipher novel plasma proteins. To address this, we applied an integrative omics pipeline to uncover novel proteins associated with JIA risk. Methods In this research, we utilized an integrative omics method to identify new plasma proteins associated with JIA. Complementary results from an independent cohort were analyzed through Whole Genome Sequencing (WGS), single-cell RNA sequencing (scRNA-seq), and bulk RNA sequencing (bulk RNA-seq) at the Children's Hospital of Chongqing Medical University to validate the reliability of the identified novel proteins. Additionally, to assess the therapeutic potential of novel proteins, we performed Phe-WAS and conducted an extensive review of existing literature using PubMed and Web of Science. Results An integrative omics pipeline analysis identified ERAP2 as having putatively causal effects on JIA. In the fourth step of Summary-data-based Mendelian randomization analysis, we discovered that the SNP rs2927608 and rs2910686 can regulate the expression of the ERAP2 gene, thereby regulating the protein content of both ERAP2 and ERAP1. WGS analysis also detected two potentially pathogenic mutations on ERAP2 in sJIA patients. ScRNA-seq reveals that ERAP2 expression is significantly elevated in patients with sJIA compared to normal and other subtypes, particularly in monocytes. Bulk RNA-seq with ROC analysis demonstrating significant diagnostic power (AUC = 0.86, 95%CI: 0.71–1.00) in discriminating sJIA from healthy controls. Literature and Phe-WAS search revealed that ERAP2 is primarily studied in the context of genetic predisposition to disease and is closely related to autoimmune disorders. Conclusions ERAP2 was identified as a candidate associated with JIA, especially sJIA, through integrative omics analysis, indicating its potential role in protein-mediated disease mechanisms and therapeutic targeting
Asymptotic behavior of solutions of Monge–Ampère equations with general perturbations of boundary values
No full textBreastfeeding duration and the risk of systemic juvenile idiopathic arthritis: a cross-sectional study
No full textBackgroundSystemic juvenile idiopathic arthritis (sJIA) is an autoinflammatory subtype of JIA with distinct immunopathogenic mechanisms. Early-life nutritional exposures such as breastfeeding may influence immune development and inflammatory disease risk, yet evidence in sJIA remains limited. Therefore, this study aimed to examine the association between breastfeeding duration and the likelihood of developing sJIA versus non-sJIA, and to assess whether systemic inflammatory markers mediate this relationship.MethodsIn this cross-sectional study, we included 450 children diagnosed with JIA from 2018 to 2024 at Children’s Hospital of Chongqing Medical University. Breastfeeding duration was retrospectively collected, and patients were classified into sJIA and non-sJIA groups. Multivariable logistic regression and restricted cubic spline models were used to assess the association between breastfeeding duration and the risk of sJIA. Mediation analysis was conducted to quantify the indirect effects of inflammatory mediators on this association.ResultsAmong 450 patients with JIA, those with sJIA (n = 150) had significantly shorter breastfeeding duration than non-sJIA patients (n = 300) (mean 6.3 vs. 9.1 months, p < 0.001). sJIA cases also showed higher levels of inflammatory markers, including neutrophil count, C-reactive protein (CRP), erythrocyte sedimentation rate, and white blood cell (WBC). Multivariable logistic regression confirmed that shorter breastfeeding duration was independently associated with higher odds of sJIA (adjusted OR = 0.86; 95% CI: 0.77–0.96; p = 0.009). Restricted cubic spline analysis revealed a non-linear inverse relationship between breastfeeding duration and sJIA likelihood, with an inflection point near 7.5 months. Mediation analysis indicated that neutrophil count, WBC count, and CRP partially mediated the relationship between breastfeeding and sJIA, accounting for 26.5, 25.8, and 12.4% of the total effect, respectively.ConclusionShorter breastfeeding duration is associated with a higher probability of sJIA, and this relationship may be partially mediated by systemic inflammatory status. These findings highlight the potential role of early-life nutritional exposures in promoting autoinflammatory disease expression and support further prospective investigations
Research on the Evolution of Development Mode of Trade Network Based on the Economic Globalization
No full text- …
