2,461 research outputs found
Estimating effects of mutations to SARS-CoV-2 proteins from natural sequences
No full textThis is Jesse Bloom's presentation at the monthly [virtual] Antiviral Drug Discovery (AViDD) Open Science Forum on 2023 February 15. You can find a recording of their talk on the AViDD Open Science Forum Youtube page. Jesse Bloom is a part of the ASAP Discovery Consortium.
Interactive slides: https://slides.com/jbloom/sars2-mut-fitness
Interactive data browser: https://jbloomlab.github.io/SARS2-mut-fitness/
Preprint: https://www.biorxiv.org/content/10.1101/2023.01.30.526314v
Lansing B. Bloom
No full textPhoto of Lansing B. Bloom, historian, professor, editor and author. Bloom was editor of the New Mexico Historical Review and UNM professor, 1926-1946
Adding Bloom to High-Dynamic-Range Tone Mapping
No full textWe present a technique for enhancing high-dynamic-range tone mapping algorithms by adding the bloom effect to bright areas. Bloom is based on the fact that real-life lenses convolve light and make bright areas emit a glow. The algorithm takes a set of images with different exposures as input, and performs a tone mapping algorithm on these. It then takes the image with the lowest exposure value to create the bloom effect. It then perform a convolution on this image with with a kernel that represents the response to one point of light. The resulting image is then added on top of the tone mapped image. We also present parameters to change the spread of the glowing effect, how bright an area needs to be to get a significant glow, and the intensity of the glow when applied. Furthermore, the kernel can be changed to create different types of glow and highlights. These things make the technique versatile and allows the photographer to customize the effect.https://github.com/ricardovogel/tonemap-and-bloom Code repositoryCSE3000 Research ProjectComputer Science and Engineerin
Influenza viruses with receptor-binding neuraminidases
No full textThesis (Ph.D.)--University of Washington, 2015University of Washington Abstract Influenza viruses with receptor-binding neuraminidases Kathryn A. Hooper Chair of the Supervisory Committee: Assistant Member Jesse D. Bloom Division of Basic Sciences and Computational Biology, Fred Hutchinson Cancer Research Center For the vast majority of influenza viruses characterized to date, the two main surface proteins have opposing and mutually exclusive functions. Hemagglutinin (HA) is the viral entry protein, mediating attachment of the virus to the host cell via sialic acid-receptors and fusion of the viral and endosomal membranes. In contrast, neuraminidase (NA) is the viral exit protein, and is a sialidase that cleaves receptors from the surface of the producing cell to facilitate viral release. In the last few years, a growing body of evidence has shown that the receptor binding and receptor cleaving activities need not be mutually exclusive, and that single amino-acid mutations can allow NA to act as the receptor-binding protein. These mutations are D151G in N2 subtype NAs and G147R in N1 subtype NAs. Here I describe the characterization of the G147R N1 NA mutation in Chapter II which was serendipitously discovered as a tissue-culture adaptation mutation. In Chapter III, I examine the effect of the G147R mutation in the background of naturally circulating viruses from three different viral lineages. I find that the receptor-binding NA mutation has no effect on the fitness of a pandemic human H1N1 virus in cell culture or in a mouse model of pathogenesis. The presence of the receptor-binding NA also does not affect sensitivity of the virus to neutralization by an anti-HA IgG, although it does slightly protect against neutralization by the Fab domain of the antibody. The physiological relevance of escape from anti-HA Fab, however, remains unclear. Overall, the identification of NA receptor-binding mutations represents a new aspect of influenza virus biology which warrants further investigation. The first NA-binding mutation was discovered when viruses failed to behave as expected in traditional hemagglutination inhibition assays. The acquisition of this D151G mutation by many recent H3N2 strains has remained a problem for the determination of vaccine efficacy in anti-sera hemagglutination inhibition assays. My work extended the finding that receptor-binding mutations can occur in N2 subtype NAs to the N1 subtype, as well. This suggests that the overall spectrum of NA-binding mutations may be much greater than is currently understood. Furthermore, because the N1 binding mutation does not significantly compromise the fitness of a recent human virus, my work also suggests that NA-binding mutations may have the potential to become more widespread in the future
The Gnostic Author of Harold Bloom
No full textČlanak isprva kontekstualizira pojavu tzv. authorship studies koje su nastale kao svojevrsna reakcija na poststrukturalističko (R. Barthes i M. Foucault) redefiniranje autorstva. U taj se kontekst zatim pozicionira Harold Bloom. On je, naime, iz uvjerenja da su poststrukturalističke antihumanističke tendencije pokrenule degenerativne procese, pa u konačnici i rastakanje književnosti u prvi plan svoje književne teorije postavio kanon i autora. Dok se o prvome znatno pisalo, drugo – pitanje autorstva – u studijama o Bloomu uglavnom je zanemareno. Stoga se u članku pozornost pridaje ponajprije Bloomovu tumačenju autora. Nastoji se pokazati da njegovo shvaćanje autorstva (kao, uostalom, i kanona) proizlazi iz gnostičke tradicije (Valentin i Lurija). Autor je za Blooma, zaključuje se, ponajprije genij – onaj koji stvara potaknut „iskrom“, pneumom, božanstvom u dnu vlastita uma, što je zapravo također stanoviti antihumanizam.The article contextualizes at first the occurrence of the so-called authorship studies that arose as a kind of reaction to the post-structuralist (R. Barthes and M. Foucault) redefinition
of authorship. Harold Bloom then positioned himself in this context. Namely, from the belief that post-structuralist anti-humanist tendencies initiated degenerative processes, and ultimately the disintegration of literature, he placed the canon and the author at the forefront of his literary theory. While much has been written about the first term, the second—the question of the authorship—has been mostly neglected in Bloom studies. Therefore, the article focuses primarily on Bloom’s interpretation of the author. The article tries to show that his understanding of authorship (as well as the canon, after all) derives from the Gnostic tradition (Valentin and Luria). For Bloom, the author is, it is concluded, primarily a genius—one who creates inspired by a “spark,” a pneuma, a divinity at the bottom of one’s own mind, which is actually also a certain form of anti-humanism
E-BLOOM: INTEGRATED WEB-BASED DATABASE SYSTEM IN MANAGING PT. BLOOM AGRO PROCUREMENT, SALES, AND INSPECTION PROCESSES
No full textE-BLOOM: INTEGRATED WEB-BASED DATABASE SYSTEM IN MANAGING PT. BLOOM AGRO PROCUREMENT, SALES, AND INSPECTION PROCESSES - Information system, web database, integrated database system
Bloom
No full textBloom details a personal journey of loss and the growth that came after it. It explores ideas such as the Abject, and those related to Embodied Geographies, Auto-ethnography, Trauma Theory, Ecology, the Rhizome, as well as in a text by Pravu Mazumdar titled Preferring Not to Die. Through these ideas the reader hopefully comes to view the wider relevance of connecting biographical experiences with ideas in order to understand a state of being that has transformed through loss and growth. Detailing the processes and different materials used throughout the physical jewellery work of Bloom allows the reader to understand the evolution of Antonia Boyle’s master’s project and its different iterations over a period of time.
A community of practice features Lucie Marjerus, Otto Kunzli, Naomi Filmer, Pierre Degen, Majorie Schick, Christoph Zellweger, Biojewellery, Hanna Hedman, Dalya Lutwak, Diana Scherer, Lauren Simeoni and Melinda Young, and Vicki Mason. Their work provides a context within which related ideas are explored via a multitude of materials.
The dissertation rounds off by detailing the importance of documentation of previous iterations of Bloom and the choice of gallery and proposed exhibition plans.
Bloom is a story of the evolution of life and how to embrace loss as a positive transitional stage. Bloom is a story of life and its cycle, a story which could be relevant to all as we all face loss of some kind or another. Bloom is a state of being which is embraced wholeheartedly by the author and this dissertation details the physical and mental processes of grief and post-traumatic growth that led to it
The decline process and major pathways of Microcystis bloom in Taihu Lake, China
No full textEutrophication has become a serious concern in many lakes, resulting in cyanobacterial blooms. However, the mechanism and pathways of cyanobacteria decline are less understood. To identify and define the growth and decline of Microcystis blooms in Taihu Lake of China, and to illuminate the destination of surface floating blooms, we investigated the biomass distribution and variations in colony size, morphology, and floating velocity from October 2008 to September 2009. The results showed that the Microcystis bloom declined in response to biomass decrease, colony disaggregation, buoyancy reduction, and increased phytoplankton biodiversity, and these indicative parameters could be applied for recognition of the development phases of the bloom. Three major decline pathways were proposed to describe the bloom decline process, colony disaggregation (Pathway I), colony settlement (Pathway II), and cell lysis in colonies (Pathway III). We proposed a strategy to define the occurrence and decline of Microcystis blooms, to evaluate the survival state under different stress conditions, and to indicate the efficiency of controlling countermeasures against algal blooms.Eutrophication has become a serious concern in many lakes, resulting in cyanobacterial blooms. However, the mechanism and pathways of cyanobacteria decline are less understood. To identify and define the growth and decline of Microcystis blooms in Taihu Lake of China, and to illuminate the destination of surface floating blooms, we investigated the biomass distribution and variations in colony size, morphology, and floating velocity from October 2008 to September 2009. The results showed that the Microcystis bloom declined in response to biomass decrease, colony disaggregation, buoyancy reduction, and increased phytoplankton biodiversity, and these indicative parameters could be applied for recognition of the development phases of the bloom. Three major decline pathways were proposed to describe the bloom decline process, colony disaggregation (Pathway I), colony settlement (Pathway II), and cell lysis in colonies (Pathway III). We proposed a strategy to define the occurrence and decline of Microcystis blooms, to evaluate the survival state under different stress conditions, and to indicate the efficiency of controlling countermeasures against algal blooms
\u3cem\u3eThe Old Man and the Sea\u3c/em\u3e
No full textReader’s companion geared to young adults. Reprints excerpts of essays from such well-known Hemingway scholars as Carlos Baker, Linda W. Wagner, Philip Young, and Bickford Sylvester. Includes a brief biography and chronology of the author’s life along with an introductory essay by Bloom in which he characterizes the novel as both “repetitive and self-indulgent.” With so little respect shown for either the author or novella, one wonders why Bloom bothered to edit the volume
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